Preparation of a Traditional Poly Herbal Gel for Abnormal Vaginal Discharge

Document Type: Original paper


1 Student Research Committee, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.


Background and objectives: Plants are usually used for treatment of different diseases according to their traditional application. The use of the plant based medications is increasingly becoming popular all over the world. Traditional herbal medicines should be transformed into new forms to make better acceptance and more comfortable use by patients. The aim of the present study was to formulate a poly herbal gel based on Iranian traditional medicine (ITM), and determine its physicochemical characteristics. Methods: The gel formulation consisted of Boswellia spp., Carum carvi L., Punica granatum L. and Myrtus communis L. extracts. The product was evaluated for the physicochemical parameters. The laboratory stability tests, microbiological tests and rheological behavior were performed on the gel. Additionally, total tannin content of the gel was measured as pyrogallol using Folin-Ciocalteu reagent and hide powder. Results: Stability and physicochemical evaluations as well as microbiological tests showed that the prepared formulation was stable towards physical changes with no growth of pathogenic microorganisms. Moreover, it represented plastic behavior which is in favor of a topical product and alsototal tannins contents were determined to be 0.27 % in the gel. Conclusion: The product was acceptable due to its physical characterisitcs and so appropriate for topical use. Regarding the antibacterial, antifungal and anti-inflammatory properties of the medicinal herbs in the gel and also, the role of tannins as astringent agents, this formulation could be a suitable candidate for abnormal vaginal discharge with regards to its traditional application in ITM.


[1] Jaradat NA, Zaid AN, Hussen F, Issa L, Altamimi M, Fuqaha B, Nawahda A, Assadi M. Phytoconstituents, antioxidant, sun protection and skin anti-wrinkle effects using four solvents fractions of the root bark of the traditional plant Alkanna tinctoria (L.). Eur J Integr Med. 2018; 21: 88-93.

[2] Kaleeswaran B, Ramadevi S, Murugesan R, Srigopalram S, Suman T, Balasubramanian T. Evaluation of anti-urolithiatic potential of ethyl acetate extract of Pedalium murex L. on struvite crystal (kidney stone). J Tradit Complement Med. 2019; 9(1): 24-37.

[3] Varghese S, Narmadha R, Gomathi D, Kalaiselvi M, Devaki K. Phytochemical screening and HPTLC finger printing analysis of Citrullus lanatus (Thunb.) seed. J Acute Dis. 2013; 2(2): 122-126.

[4] Kumar V, Parmar NS. Herbs: a potential source for the development of new pytomedicinals. Pharm Rev. 2003; 1(4): 59-63.

[5] Seeff LB, Curto TM, Szabo G, Everson GT, Bonkovsky HL, Dienstag JL, Shiffman ML, Lindsay KL, Lok AS, Di Bisceglie AM, Lee WM. Herbal product use by persons enrolled in the hepatitis C Antiviral Long‐Term Treatment Against Cirrhosis (HALT‐C) Trial. Hepatology. 2008; 47(2): 605-612.

[6] McCathie R. Vaginal discharge: common causes and management. Curr Obstet Gynecol Rep.2006; 16(4): 211-217.

[7] Ilankoon I, Goonewardena C, Fernandopulle R, Perera P. An insight to how estate women in Sri Lanka manage abnormal vaginal discharge. Iran J Public Health. 2017; 46(2): 268–269.

[8] Meena V, Bansal CL. Study to evaluate targeted management and syndromic management in women presenting with abnormal vaginal discharge. J Obstet Gynaecol India. 2016; 66(1): 534-540.

[9] Nwadioha S, Egesie JO, Emejuo H, Iheanacho E. Prevalence of pathogens of abnormal vaginal discharges in a Nigerian tertiary hospital. Asian Pac J Trop Med. 2010; 3(6): 483-485.

[10] Nazem Jahan MA. Exir-e-azam. Tehran: Iran University of Medical Sciences, 2008.

[11] Aghili Alavi-Shirazi MH. Gharabadin-e-kabir. Tehran: Tehran University of Medical Sciences, 2007.

[12] Nazem Jahan MA. Gharabadin-e-azam. 2nd ed. Tehran: Iran University of Medical Sciences, 2004.

[13] Misal G, Dixit G, Gulkari V. Formulation and evaluation of herbal gel. Indian J Nat Prod Resour. 2012; 3(4): 501-505.

[14] Loganathan V, Manimaran S, Jaswanth A. The effects of polymers and permeation enhancers on releases of flurbiprofen from gel formulations. Indian J Pharm Sci. 2001; 63(3): 200-204.

[15] The British Pharmacopoeia Commission. British pharmacopoeia. London: The Stationary Office, 2011.

[16] Iranian Herbal Pharmacopoeia Committee. Iranian Herbal Pharmacopoeia. 1st ed. Tehran: Ministry of Health and Medical Education of Iran, Food and Drug Administration, 2002.

[17] Fahimi S, Mortazavi SA,  Abdollahi M, Hajimehdipoor H. Formulation of a traditionally used polyherbal product for burn healing and HPTLC fingerprinting of its phenolic contents. Iran JPharm Res. 2016; 15(1): 95-105.

[18] World Health Organization. Quality control methods for herbal materials. Geneva: WHO Press, 2011.

[19] Khan AW, Kotta S, Ansari SH, Sharma RK, Kumar A, Ali J. Formulation development, optimization and evaluation of Aloe vera gel for wound healing. Pharmacogn Mag. 2013; 9(1): 6-10.

[20] Krepps SI. Sustain preparation in: cosmetic science and technology. USA: Krieger Publishing Compony, 1992.

[21] Lee CH, Moturi V, Lee Y. Thixotropic property in pharmaceutical formulations. J Control Release. 2009; 136(2): 88-98.

[22] Mortazavi SA, Jafari azar Z. Formulation and in-vitro evaluation of tretinoin microemulsion as a potential carrier for dermal drug delivery. Iran J Pharm Res. 2013; 12(4): 599-609.

[23] Korhonen M, Niskanen H, Kiesvaara J, Yliruusi J. Determination of optimal combination of surfactants in creams using rheology measurements. Int J Pharm. 2000; 197(1): 143–151.

[24] Mortazavi SA, Tabandeh H. The influence of various silicones on the rheological parameters of AZG containing silicone-based gels. Iran J Pharm Res. 2005; 20(4): 205-211.

[25] Jahandideh M, Hajimehdipoor H, Mortazavi SA, Dehpour A, Hassanzadeh G. A wound healing formulation based on iranian traditional medicine and its HPTLC fingerprint. Iran J Pharm Res. 2016; 15 (S): 149-157.

[26] Mehrabani M, Kazemi A, Ayatollahi Mousavi SA, Rezaifar M, Alikhah H, Nosky A. Evaluation of antifungal activities of Myrtus communis L. by bioautography method. Jundishapur J Microbiol. 2013; 6(8): 1-7.

[27] Belmimoun A, Meddah B, Meddah A, Sonnet P. Antibacterial and antioxidant activities of the essential oils and phenolic extracts of Myrtus communis and Zygophylum album from Algeria. J Fundam Appl Sci. 2016; 8(2): 510-524.

[28] Amensour M, Bouhdid S, Fernández-López J, Idaomar M, Senhaji NS, Abrini J. Antibacterial activity of extracts of Myrtus communis against food-borne pathogenic and spoilage bacteria. Int J Food Prop. 2010; 13(6): 1215-1224.

[29] Hennia A, Brada M, Nemmiche S, Fauconnier ML, Lognay G. Chemical composition and antibacterial activitiy of the essential oils of Algerian Myrtus communis L. J Essent Oil Res. 2015; 27(4): 324-328.

[30] Ozcan MM, Uyar B, Unver A. Antibacterial effect of myrtle (Myrtus communis L.) leaves extract on microorganisms. J FoodSafety Food Qual. 2015; 166(1): 8-21.

[31] Eslami G, Hashemi A, Yazdi MM, Benvidi ME, Rad PK, Moradi SL, Fallah F, Dadashi M. Antibacterial effects of Zataria multiflora, Ziziphus, chamomile and Myrtus communis methanolic extracts on IMP-type metallo-beta-lactamase-producing Pseudomonas aeruginosa. Arch Clin Infect Dis. 2016;11(1): Article ID 32413.

[32] Cannas S, Molicotti P, Usai D, Maxia A, Zanetti S. Antifungal, anti-biofilm and adhesion activity of the essential oil of Myrtus communis L. against Candida species. Nat Prod Res. 2014; 28(23): 2173-2177.

[33] Yilar M, Bayan Y, Onaran A. Chemical composition and antifungal effects of Vitex agnus-castus L. and Myrtus communis L. plants. Not Bot Horti Agrobo. 2016; 44(2): 466-471.

[34] Mahdi N, Gany Z, Sharief M. Alternative drugs against Trichomonas vaginalis. East Mediterr Health J. 2006; 12(‎5)‎: 679-684.

[35] Tumen I, Akkol EK, Suntar I, Erbey G, Kurtca M, Keles H, Reunanen M, Pranovich A. Evaluation of the wound healing and anti-inflammatory activities and phytochemical analysis of Myrtus communis L. Fresenius Envir Bull. 2017; 26(7): 4420-4428.

[36] Rossi A, Di Paola R, Mazzon E, Genovese T, Caminiti R, Bramanti P, Pergola C, Koeberle A, Werz O, Sautebin L, Cuzzocrea S. Myrtucommulone from Myrtus communis exhibits potent anti-inflammatory effectiveness in vivo. J Pharmacol Exp Ther. 2009; 329(1): 76-86.

[37] Feißt C, Franke L, Appendino G, Werz O. Identification of molecular targets of the oligomeric nonprenylated acylphloroglucinols from Myrtus communis and their implication as anti-inflammatory compounds. J Pharmacol Exp Ther. 2005; 315(1): 389-396.

[38] Ozturk S, Canbay E, Yildirim H, Sozmen E. Phenolic content and potential anti-inflammatory effects of Myrtus communis L. products. Fresenius Envir Bull. 2016; 283(1): 74-78.

[39] Li Y, Guo C, Yang J, Wei J, Xu J, Cheng S. Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chem. 2006; 96(2): 254-260.

[40] Viuda‐Martos M, Fernández‐López J, Pérez‐Álvarez J. Pomegranate and its many functional components as related to human health: a review. Compr Rev Food Sci Food Saf. 2010; 9(6): 635-654.

[41] Gullon B, Pintado ME, Pérez-Álvarez JA, Viuda-Martos M. Assessment of polyphenolic profile and antibacterial activity of pomegranate peel (Punica granatum) flour obtained from co-product of juice extraction. Food Control. 2016; 59: 94-98.

[42] Colak M. Determination of antifungal and antibacterial activities of natural dye of Pomegranate skin (Punica granatum L.) implemented on wooden materials. Wood Res. 2016; 61(5): 709-717.

[43] El-Sherbini G, Ibrahim K, El Sherbini E, Hady NA, Morsy T. Efficacy of Punica granatum extract on in vitro and in vivo control of Trichomonas vaginalis. J Egypt Soc Parasitol. 2010; 40(1): 229-244.

[44] El-Sherbini G, El Gozamy B, Abdel-Hady N, Morsy T. Efficacy of two plant extracts against vaginal trichomoniasis. J Egyp Soc Parasitol. 2009; 39(1): 47-58.

[45] Lee CJ, Chen LG, Liang WL, Wang CC. Anti-inflammatory effects of Punica granatum Linne invitro and in vivo. Food Chem. 2010; 118(2): 315-322.

[46] Houston DM, Bugert J, Denyer SP, Heard CM. Anti-inflammatory activity of Punica granatum L. (Pomegranate) rind extracts applied topically to ex vivo skin. Eur J Pharm Biopharm. 2017; 112: 30-37.

[47] BenSaad LA, Kim KH, Quah CC, Kim WR, Shahimi M. Anti-inflammatory potential of ellagic acid, gallic acid and punicalagin A&B isolated from Punica granatum. BMC Complement Altern Med. 2017; 17(1): 47-56.

[48] Marichali A, Hosni K, Dallali S. Allelopathic effects of Carum carvi L. essential oil on germination and seedling growth of wheat, maize, flax and canary grass.Allelopathy J. 2014; 34(1): 81-93.

[49] Thies W. HPLC of essential oils (Lavander, Peppermint and Caraway). Fresenius J Anal Chem. 1984; 318(3): 249-250.

[50] Iacobellis NS, Lo Cantore P, Capasso F, Senatore F. Antibacterial activity of Cuminum cyminum L. and Carum carvi L. essential oils. J Agric Food Chem. 2005; 53(1): 57-61.

[51] Gupta A, Dubey M, Parmar M, Mahajan S, Sharma R. Evaluation of antimicrobial activity of Carum carvi (seeds) extract against E. coli and Aspergillus niger. Drug Invent Today. 2011; 3(9): 211-213.

[52] Deb Roy S, Thakur S, Negi A, Kumari M, Sutar N, Jana GK. In vitro antibiotic activity of volatile oils of Carum carvi and Coriandrum sativum. Int J Chem Analyt Sci. 2010; 1: 149-150.

[53] De Martino L, De Feo V, Fratianni F, Nazzaro F. Chemistry, antioxidant, antibacterial and antifungal activities of volatile oils and their components. Nat Prod Commun. 2009; 4(12): 1741-1750.

[54] Škrinjar MM, Mandić AI, Mišan AČ, Sakač MB, Šarić LĆ, Zec MM. Effect of mint (Mentha piperita L.) and caraway (Carum carvi L.) on the growth of some toxigenic Aspergillus species and aflatoxin B1 production. ZB Matitse Srp Prir Nauke. 2009; 116: 131-139.

[55] Kopalli SR, Koppula S. Carum carvi Linn (Umbelliferae) attenuates lipopolysaccharide-induced neuroinflammatory responses via regulation of NF-κB signaling in BV-2 microglia. Trop J Pharm Res. 2015; 14(6): 1041-1047.

[56] Nwinyi FC, Binda L, Ajoku GA, Aniagu SO, Enwerem NM, Orisandipe A, Kubmarawa D, Gamaniel KS. Evaluation of the aqueous extract of Boswellia dalzielii stem bark for antimicrobial activities and gastrointestinal effects. Afr J Biotechnol. 2004; 3(5): 284-288.

[57] Afsar V, Reddy YM, Saritha K. Invitro antioxidant activity and anti-inflammatory activity of methanolic leaf extract of Boswellia serrata. Int J LifeSc Bt & Pharm Res. 2012; 1(4): 15-23.

[58] Banno N, Akihisa T, Yasukawa K, Tokuda H, Tabata K, Nakamura Y, Nishimura R, Kimura Y, Suzuki T. Anti-inflammatory activities of the triterpene acids from the resin of Boswellia carteri. J Ethnopharmacol. 2006; 107(2): 249-253.

[59] Mothana RA. Anti-inflammatory, antinociceptive and antioxidant activities of the endemic Soqotraen Boswellia elongata Balf. f. and Jatropha unicostata Balf. f. in different experimental models. Food Chem Toxicol. 2011; 49(10): 2594-2599.

[60] Van Vuuren S, Kamatou G, Viljoen A. Volatile composition and antimicrobial activity of twenty commercial frankincense essential oil samples. S Afr J Bot. 2010; 76(4): 686-691.

[61] Adelakun E, Finbar E, Agina S, Makinde A.  Antimicrobial activity of Boswellia dalziellii stem bark. Fitoterapia. 2001; 72(7): 822-824.

[62] Okwu DE, Josiah C. Evaluation of the chemical composition of two Nigerian medicinal plants. Afr J Biotechnol. 2006; 5(4): 357-361.

[63] Kim H, Banerjee N, Ivanov I, Pfent CM, Prudhomme KR, Bisson WH, Dashwood RH, Talcott ST, Mertens‐Talcott SU. Comparison of anti‐inflammatory mechanisms of mango (Mangifera Indica L.) and pomegranate (Punica Granatum L.) in a preclinical model of colitis. MolNutr Food Res. 2016; 60(9): 1912-1923.

[64] Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. Tannins and human health: a review. Crit Rev Food Sci Nutr. 1998; 38(6): 421-464.