Evaluation of Anti-Nociceptive and Anti-Inflammatory Activities of Apium graveolens L. Roots Extract in Mice

Document Type: Original paper

Authors

1 Department of Pharmacognosy, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran. Herbal Medicine Center, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

2 Herbal Medicine Center, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

3 Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

4 Department of Pharmaceutics, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

5 Department of Pharmacognosy, Faculty of Pharmacy, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abstract

Background and objectives: Pain is an unpleasant feeling which affects the quality of life and relieving from pain is an important goal in many treatment protocols. Apium graveolens L. (celery) has been considered as sedative, analgesic, carminative, antispasmodic and diuretic in Iranian traditional medicine. The aim of present study was to evaluate the anti-nociceptive and anti-inflammatory effect of celery root in mice. Method: Repeated maceration was employed as extraction method. Hot plate and acetic acid writhing test were conducted to assess analgesic effect of celery root. The extract was also evaluated for anti-inflammatory probable effect by formalin induced ear edema and xylene induced paw edema tests. Results: Total flavonoid content determined by aluminum chloride colorimetric method was 0.0625 mg quercetin/g extract. No significant difference was observed between the positive control group which received morphine and test group in hot plate test and the most effective dose of celery root extract was 200 mg/kg, while the frequency of writhings was significantly different in all test groups in comparison with control group (p value< 0.05), the extract (100, 200 and 400 mg/kg) significantly suppressed inflammation in formalin induced edema assay, 60 and 120 min after injection. The results of xylene test also demonstrated notable antiedematogenic effect in applying 200 and 400 mg/kg of extract. Conclusion: Celery root has analgesic and anti-inflammatory effects which might be related to the flavonoids and resins present in the species.
 

Keywords


[1] American Academy of Pain Medicine. Facts and figures on pain. [Accessed 2018]. Available from: http://www.painmed.org/patientcenter/facts_on_pain.aspx.

[2] Kilic FS, Sirmagul B, Yildirim E, Oner S, Erol K. Antinociceptive effects of gabapentin & its mechanism of action in experimental animal studies. Indian J Med Res. 2012; 135(5): 630-635.

[3] Rates SMK. Plants as source of drugs. Toxicon. 2001; 39(5): 603-613.

[4] Vovlas N, Lucarelli G, Sasanelli N, Troccoli A, Papajova I, Palomares‐Rius JE, Castillo P. Pathogenicity and host‐parasite relationships of the root‐knot nematode meloidogyne incognita on celery. Plant Pathol J. 2008; 57(5): 981-987.

[5] Blumenthal M. Commission E. Herbal medicine: expanded Commission E monographs. Newton:Integrative Medicine Communications, 2000.

[6] Mencherini T, Cau A, Bianco G, Loggia RD, Aquino RP, Autore G. ‏An extract of Apium                 graveolens var. dulce leaves: structure of the major constituent, apiin, and its anti‐inflammatory properties. J Pharm Pharmacol. 2007; 59(6): 891-897.

[7] Abdel-Moein NM, Abdel-Moniem EA, Mohamed DA, Hanfy EA. Evaluation of the anti-inflammatory and anti-arthritic effects of some plant extracts. Grasas Aceites. 2011; 62(4): 365-374.

[8] Nasri S, Shahi SF, Kamalinejad M, Rabbani T. Investigation of the possible mechanism of antinociceptive effect of Apium graveolens hydroalcoholic fruits extract. Arak Med Univ J. 2012; 15(5): 66-75.

[9] Abdou HS, Salah SH, Booles H, EA AR. Antioxidant effect of celery against carbontetrachloride induced hepatic damage in rats. Afr J Microbiol Res. 2012; 6(27): 5657-5667.

[10] Sipailiene A, Venskutonis PR, Sarkinas A, Cypiene V. Composition and antimicrobial activity of celery (Apium graveolens) leaf and root extracts obtained with liquid carbon dioxide. Acta Hortic. 2015; 677: 71-77

[11] Ahmed B, Alam T, Varshney M, Khan SA. Hepatoprotective activity of two plants belonging to the Apiaceae and the Euphorbiaceae family. J Ethnopharmacol. 2002; 79(3): 313-316.

[12] Singh A, Handa SS. Hepatoprotective activity of Apium graveolens and Hygrophila auriculata against paracetamol and thioacetamide intoxication in rats. J Ethnopharmacol. 1995; 49(3): 119-126.

[13] Al-Howiriny T, Alsheikh A, Alqasoumi S, Al-Yahya M, ElTahir K, Rafatullah S. Gastric antiulcer, antisecretory and cytoprotective properties of celery (Apium graveolens) in rats. Pharm Biol. 2010; 48(7): 786-793.

[14] Kolarovic J, Popovic M, Mikov M, Mitic R, Gvozdenovic L. Protective effects of celery juice in treatments with doxorubicin. Molecules. 2009; 14(4): 1627-1638.

[15] Al-Antaki D. Tathkirat Daoud Al-Antaki. 1st ed. Beyrut: Moaseat-ol-elmi-al-matbooat.

[16] Wolski T, Dyduch J. Importance of vegetables and fruit in civilization-related therapy. Agris. 2004; 8: 19-38.

[17] Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983; 16(2): 109-110.

[18] Ardekani MRS, Hajimahmoodi M, Oveisi MR, Sadeghi N, Jannat B, Ranjbar AM, Gholam N, Moridi T. Comparative antioxidant activity and total flavonoid content of Persian pomegranate (Punica granatum L.) cultivars. Iran J Pharm Res. 2011; 10(3): 519-524.

[19] Bagheri SM, Dashti RMH, Morshedi A. Antinociceptive effect of Ferula assa-foetida oleo-gum-resin in mice. Res Pharm Sci. 2014; 9(3): 207-212.

[20] Collier HOJ, Dinneen LC, Johnson CA, Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. Br J Pharmacol Chemother. 1968; 32(2): 295-310.

[21] Hosseinzadeh H, Ramezani M, Salmani GA. Antinociceptive, anti-inflammatory and acute toxicity effects of Zataria multiflora Boiss. extracts in mice and rats. J Ethnopharmacol. 2000; 73(3): 379-385.

[22] Ramezani M, Nasri S, Yassa N. Antinociceptive and anti-inflammatory effects of isolated fractions from Apium graveolens seeds in mice. Pharm Biol. 2009; 47(8): 740-743.

[23] Atta AH, Alkofahi A. Anti-nociceptive and anti-inflammatory effects of some Jordanian medicinal plant extracts. J Ethnopharmacol. 1998; 60(2): 117-124.

[24] Odontuya G, Hoult JRS, Houghton PJ. Structure‐activity relationship for antiinflammatory effect of luteolin and its derived glycosides. Phytother Res. 2005; 19(9): 782-786.

[25] Tjolsen A, Berge OG, Hunskaar S, Rosland JH, Hole K.  The formalin test: an evaluation of the        method. Pain. 1992; 51(1): 5-17.

[26] Rotelli AE, Guardia T, Juárez AO, De la Rocha NE, Pelzer LE. Comparative study of flavonoids in experimental models of inflammation. Pharmacol Res. 2003; 48(6): 601-606.

[27] Lewis DA, Tharib SM, Veitch GBA. The anti-inflammatory activity of celery Apium graveolens L.(fam. Umbelliferae). Pharm Biol. 1985; 23(1): 27-32.

[28] Ovodova RG, Golovchenko VV, Popov SV, Popova GY, Paderin NM, Shashkov AS, Ovodov YS. Chemical composition and anti-inflammatory activity of pectic polysaccharide isolated from celery stalks. Food Chem. 2009; 114(2): 610-615.

[29] Kooti W, Ali-Akbari S, Asadi-Samani M, Ghadery H, Ashtary-Larky D. A review on medicinal plant of Apium graveolens. Adv Herb Med. 2015; 1(1): 48-59.