Vitex doniana, In-Vitro Antioxidant, Membrane Stabilization Potential and Protective Impact Against Plasmodium berghei-Passaged Mice

Document Type : Original paper

Authors

1 Department of Biochemistry, College of Biological Sciences, Federal University of Agriculture, Makurdi, Nigeria.

2 Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Nigeria.

3 School of Nutrition Sciences, Faculty of Health Sciences, University of Ottawa, Ontario, Canada.

4 Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka, Nigeria.

Abstract

Background and objectives: Vitex doniana Sweet (Lamiaceae) is used to treat various ailments, including respiratory infections, liver diseases, anaemia and jaundice. This study assessed the in vitro antioxidant and membrane stabilization potential, as well as the protective impact of semi-purified solvent fractions of V. doniana leaves against Plasmodium berghei-passaged mice. Methods: Dried leaves were extracted with ethanol, followed by fractionation using a solvent-gradient system of increasing polarity (hexane, ethyl acetate, and methanol), and the concentrated fractions were obtained. Forty-two mice were randomly divided into seven groups as: group 1 (normal control), group 2 (disease control, untreated), while groups 3 to 7 received the standard drugs (artequick and chloroquine) and combined V. doniana fraction (VDF, 100 mg/kg) at varying ratios. Results: Comparatively to V. doniana extract, the fractions (F6, F8) displayed considerable antioxidant activity by scavenging O2•–, OH• and DPPH radicals, and effectively reduced Fe3+ to Fe2+. VDF (1:1) at different concentrations (200, 400, 600 µg/mL) inhibited erythrocyte haemolysis by 91.29±3.61%, 80.52±0.13%, 75.68±1.45% and 80.57±0.94%, respectively. Also, the VDF in synergy with artequick and chloroquine decreased parasitaemia levels by 4.25±0.25% and 4.65±0.28% compared to the disease control (7.93±1.61%). The combined fractions significantly normalized the plasma calcium concentration (1.85±0.17 mg/dL, 1.65±0.21 mg/dL, 1.72±0.23 mg/dL, 1.65±0.22 mg/dL) for groups 3 to 6 compared to the disease control (1.30±0.09 mg/dL), while the bodyweights presented no significant change in all experimental groups. Conclusion: The results indicate the promising potential of V. doniana as a drug candidate in managing malarial infection.

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Main Subjects

References

  • Burda PC, Caldelari R, Heussler VT. Manipulation of the host cell membrane during Plasmodium liver stage egress. mBio. 2017; 8(2): 1–12.
  • Arora N, Anbalagan LC, Pannu AK. Towards eradication of malaria: is the WHO's RTS,S/AS01 vaccination effective enough? Risk Manag Healthc Policy. 2021; 14: 1033–1039.
  • Al-Awadhi M, Ahmad S, Iqbal J. Current status and the epidemiology of malaria in the Middle East region and beyond. Microorganisms. 2021; 9(2): 1–20.
  • World Health Organization. World malaria report: 20 years of global progress and challenges. Geneva: World Health Organization,
  • Okpe O, Agada EE, Okoduwa SIR, Upev VA, Micheal AS. Aqueous stem bark of Mangifera indica suppresses parasitemia and ameliorates anaemia in mice model of malarial infection. Maleys J Biochem Mol Biol. 2021; 3(12): 105–112.
  • Gomes ARQ, Cunha N, Varela ELP, Brígido HPC, Vale VV, Dolabela MF, de Carvalho EP, Percário S. Oxidative stress in malaria: potential benefits of antioxidant therapy. Int J Mol 2022; 23(11): 1–26.
  • Babalola AS, Jonathan J, Michael BE. Oxidative stress and antioxidants in asymptomatic malaria-positive patients: a hospital-based cross-sectional Nigerian study. Egypt J Intern Med. 2020; 23(23): 1–8.
  • Gómez X, Sanon S, Zambrano K, Asquel S, Bassantes M, Morales JE, Otáñez G, Pomaquero C, Villarroel S, Zurita A, Calvache C, Celi K, Contreras T, Corrales D, Naciph MB, Peña J, Caicedo A. Key points for the development of antioxidant cocktails to prevent cellular stress and damage caused by reactive oxygen species (ROS) during manned space missions. NPJ Microgravity. 2021; 7(35): 1–19.
  • Arrey Tarkang AP, Nwachiban Atchan AP, Kuiate JR, Okalebo FA, Guantai AN, Agbor GA. Antioxidant potential of a polyherbal antimalarial as an indicator of its therapeutic value. Adv Pharmacol Sci. 2013; Article ID 78458.
  • Hagazy K, Sibhat GG, Karim A, Tekulu GH, Periasamy G, Hiben GM. Antimalarial activity of Meriandra dianthera leaf extracts in Plasmodium berghei-infected mice. Evid Based Complement Altern Med. 2020; Article ID 8980212.
  • Oche O, Uche SN, John O, Samuel MR, Esin EE, David AD, Catherine EO. GC–MS profiling and antimalarial activity of Khaya grandifoliola on Plasmodium berghei-infected mice. J Herbs Spices Med Plants. 2018; 25(1): 21–32.
  • Lawal B, Shittu OK, Kabiru AY, Jigam AA, Umar MB, Berinyuy EB, Aloziuwa BU. Potential antimalarials from African natural products: a review. J Intercult Ethnopharmacol. 2015; 4(4): 318–343.
  • Sofowora EA. Medical plant and traditional remedies in African. 2nd Ibadan: University of lle-lfe Press, 1993.
  • Beentje HJ. Kenya trees, shrubs and lianas. Nairobi: National Museums of Kenya, 1994.
  • Iwueke AV, Nwodo OFC, Okoli CO. Evaluation of the anti-inflammatory and analgesic activities of Vitex doniana Afr J Biotechnol. 2006; 5(20): 1929–1935.
  • Okpe O, Ibrahim S, Njoku GC, Ndidi US, Atabo S. Pancreatic islet regeneration and some liver biochemical parameters of leaf extracts of Vitex doniana (Verbenaceae) in normal and STZ induced diabetic albino rats. Asian Pac J Trop Biomed. 2014; 4(2): 124–130.
  • Yakubu OE, Ojogbane E, Nwodo OFC, Nwaneri-Chidozie VO, Dasofunjo K. Comparative antioxidant and hypoglycaemic effects of aqueous, ethanol and n-hexane extracts of leaf of Vitex doniana on streptozotocin-induced diabetes in albino rats. Afr J Biotechnol. 2013; 12(40): 5933–5940.
  • Ibrahim S, Okpe O, Njoku G, Ndidi US. Antihyperglycaemic and antihyperlipidaemic effects of ethanolic and aqueous leaf extract of Vitex doniana (Verbenaceae) on streptozotocin-induced diabetic Albino rats. Res J Med Plant. 2014; 8(4): 178–186.
  • Sasidharan S, Chen Y, Saravanan D, Sundran KM, Yoga Y. Extraction, isolation and characterization of bioactive compounds from plants' extracts. Afr J Tradit Complement Altern Med. 2011; 8(1): 1–10.
  • Aliyu AB, Achika JI, Adewuyi JA, Gangas P, Ibrahim H, Oyewale AO. Antioxidants from Nigerian medicinal plants: What are the evidence? [Accessed 2022] Available from: https://www.com/chapters/67162.
  • Mahalik G, Jali P, Sahoo S, Satapathy K. Ethnomedicinal, phytochemical and pharmacological properties of Mangifera indica Int J Botany. 2020; 59(2): 1–5.
  • Chowdhury A, Alam MA, Rahman NS, Hassan MA, Rashid MA. Antioxidant, antimicrobial and cytotoxic activities of Corypha taliera Lat Am J Pharm. 2020; 29(7): 1231–1234.
  • Kavimani S, Saminathan K, Senthil KR. Antioxidant and free radical scavenging activities of Dolichandrone atrovirens using various in vitro assay models. Int J Phytopharm. 2014; 5(4): 293–300.
  • Gulcin I, Huyut Z, Elmastas M, Aboul-Enein HY. Radical scavenging and antioxidant activity of tannic acid. Arab J Chem. 2010; 3(1): 43–53.
  • Benzie IF, Strain J. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1): 70–76.
  • Shinde UA, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilizing activity- a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. 1999; 70(3): 251–257.
  • Ibezim A, Ofokansi MN, Ndukwe X, Chiama CS, Obi BC, Isiogugu ON, Ikechukwu PE, Onwuka AM, Ihim SA, Asegbeloyin JN, Nwodo NJ. Evaluation of anti-malarial potency of new pyrazole hydrazine coupled to Schiff base derivatives. Malar J. 2022; 21(243): 1–9.
  • Iwalokun BA. Enhanced antimalarial effects of chloroquine by aqueous Vernonia amygdalina leaf extract in mice infected with chloroquine resistant and sensitive Plasmodium berghei Afr Health Sci. 2008; 8(1): 25–35.
  • Connerty HV, Briggs AR. Determination of serum calcium by the means of sodium alizarin sulfonate. Clin Chem. 1965; 11(7): 716–728.
  • Mgbemena IC, Ifeyinwa C. The efficacy of antioxidant (vitamin C) in the treatment and management of malaria. Adv Biotech Micro. 2020; 15(4): 97–103.
  • Gazdik Z, Krska B, Adam V, Saloun J, Pokorna T. Electrochemical determination of the antioxidant potential of some less common fruit species. Sensors. 2008; 8(12): 7564–7570.
  • Soare JR, Dinis TCP, Cunha AP, Almeida LM. Antioxidant activity of some extracts of Thymus zygis. Free Radic Res. 1997; 26(5): 469–478.
  • Halliwell B, Gutteridge JMC. Free radicals in biology and medicine, 4th Oxford: Claredon Press, 2007.
  • Sakanaka S, Tachibana Y, Okada Y. Preparation and antioxidant properties of extracts of Japanese persimmon leaf tea (Ka-Kiniha-Cha). Food Chem. 2005; 89(4): 569–575.
  • Dorman HJD, Peltoketo A, Hiltunen R, Tikkanen MJ. Characterization of the antioxidant properties of deodorized aqueous extracts from selected Lamiaceae Food Chem. 2003; 83(2): 255–262.
  • Igbokwe NA. A review of the factors that influence erythrocyte osmotic fragility. Sokoto J Vet Sci. 2018; 16(4): 1–23.
  • Sharma B, Rai DK, Rai PK, Rizvi SI, Watal G. Determination of erythrocyte fragility as a marker of pesticide-induced membrane oxidative damage. Methods Mol 2010; 594: 123–128.
  • Safeukui I. Retention of Plasmodium falciparum ring-infected erythrocytes in the slow, open microcirculation of the human spleen. Blood. 2008; 112(6): 2520–2528.
  • Lew VL, Macdonald L, Ginsburg H, Krugliak M, Tiffert T. Excess haemoglobin digestion by malaria parasites: a strategy to prevent premature host cell lysis. Blood Cells Mol Dis. 2004; 32(3): 353–359.
  • Buffet PA. The pathogenesis of Plasmodium falciparum malaria in humans: insights from splenic physiology. Blood. 2011; 117(2): 381–392.
  • Anosike CA, Obidoa O, Ezeanyika LUS. Membrane stabilization as a mechanism of the anti- inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU J Pharm Sci. 2012; 20(1): 1–7.
  • Amin C, Shofiul A, Mohammed AJ, Kazi OF, Atiqul I. Antibacterial activities and in vitro anti- inflammatory (membrane stability) properties of methanolic extracts of Gardenia coronaria Int J Microbiol. 2014; Article ID 410935.
  • Ogbomade RS, Asara AA, Eboh AS. In vitro anti-inflammatory properties of leaf extract of Polyalthia longifolia Pharm Chem J. 2019; 29(6): 112–115.
  • Mathieu B, Oliver B. Calcium signalling in malaria parasites. Mol Microbiol. 2016; 100(3): 397–408.
  • Golvan J. Elements in medical parasitology. 4th Paris: Flammarion Médecine-Sciences, 1983.
  • Carafoli E. Intracellular calcium homeostasis. Annu Rev Biochem. 1987; 56: 395–433.
  • Glennon MC, Bird GJ, Kwan CY, Putney JW. Actions of vasopressin and the Ca2+-ATPase inhibitor, thapsigargin, on Ca2+ signaling in hepatocytes. J Biol Chem. 1992; 267(12): 8230–8233.
Research Journal of Pharmacognosy
Volume 10, Issue 3
July 2023
Pages 15-23

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