Pistachio Extract Improves Neurocognitive Behaviors in Ovariectomized Mice

Document Type : Original paper


1 Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

2 Student Research Committee, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.

3 Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran.

4 Physiology-Pharmacology Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.


Background and objectives: Menopause is associated with depression as well as emotional and memory disorders. Based on the anti-inflammatory and antioxidant effects of pistachio, its effect on depression, cognitive function, anxiety and physical power in ovariectomized mice was investigated. Methods: In the current study, fifty female mice were used. They were aliquoted into five groups: control, ovariectomy (OVX), ovariectomy + DMSO, ovariectomy +10 mg/kg pistachioextract and Ovariectomy +100 mg/kg pistachio extract. In order to prepare the required extract, pistachio nuts were powdered (100 g) and macerated in 1 L of ethanol (80%) for 72 h. Pistachio extract was used orally once a day in ovariectomized mice for sixty days. Anxiety, depression, working memory and physical power were evaluated by the Elevated plus-maze (EPM) test, Forced swimming test (FST), Y maze and swimming exhaustion test, respectively. Results: The results showed that extract of pistachio (more potentially at the dose of 100 mg/kg) decreased anxiety-like behaviors and depression; besides, increase in working memory and physical power was observed in the ovariectomized mice. Conclusion: The findings of the current investigation suggest that pistachio extract could be used as a potential strategy for the attenuation of ovariectomy-related manifestation


Main Subjects

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