Antioxidant Activity and Cardioprotective Effect of Potentilla reptans L. via Ischemic Preconditioning (IPC)

Document Type : Original paper

Authors

1 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

2 Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

3 Department of Pharmacology, Faculty of Pharmacy, University of Lisbon, Portugal.

4 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Abstract

Background and objectives:  Potentilla reptans L. from Rosaceae family is used as traditional medicine in Iran and other countries. Previous investigations on Potentilla species have reported strong antioxidant activity and cardioprotective effect. In this study, antioxidant activity of aerial parts and root of Potentilla reptans, and the cardio protective role of its root on preconditioning ischemia reperfusion injury have been investigated. Methods: Antioxidant activity of aerial parts and root of this plant were measured by DPPH and FRAP methods and its total phenolics content was estimated by Folin-Ciocalteu assay. Catechin was isolated from ethyl acetate fraction by Paper chromatography. Cardioprotective role of P. reptans root were evaluated by thirty five rats in five groups.The hearts were subjected to 30 minutes of ischemia and 100 minutes of reperfusion. The ischemic preconditioning (IPC) protocol was applied before the main ischemia. The myocardial infarct size was estimated by triphenyltetrazolium chloride (TTC) staining. The hemodynamic parameters, arrhythmia scoring and coronary flow were measured during reperfusion. Results: Potentilla reptans root showed stronger antioxidant activity and total phenolics content compared to the aerial parts. Total extract of root significantly decreased the infarct size and increased coronary flow in a concentration-dependent manner. Conclusion: Our results showed that the protective effects of Potentilla reptans root appeared by its phenolic compounds and reactive oxygen species (ROS) inhibition mechanism.

Keywords

Main Subjects

References

[1] Ertter B, Attar F. Changes to Potentilla s.l. (Rosaceae) in Flora Iranica. Rostaniha. 2007; 7(S2): 299-314.
[2] Faghir MB, Attar F, Ertter B.  Foliar anatomy of the genus Potentilla L. (Rosaceae) in Iran and its taxonomic implication. Iran J Sci Technol.2011; 35(3): 243-256.
[3] Schiman-Czeika H. Potentilla (Rosaceae). In: Flora Iranica (ed. Rechinger, K.H.). Graz: Akademische Druck-u Verlagsanstalt, 1969.
[4] Chaoluan L, Ikeda H, Ohba H. Potentilla Linnaeus. Flora of China. 2003; 9: 291.
[5] De Natale A, Pollio A. Plant’s species in the folk medicine of Montecorvino Rovella (inland Campania, Italy). J Ethnopharmacol. 2007; 109(2): 295-303.
[6] Tomczyk M, Latté KP. Potentilla- a review of its phytochemical and pharmacological profile. J Ethnopharmacol. 2009; 122(2): 184-204.
[7] Gurbuz I, Ozkan AM, Yesilada E, Kutsal O. Anti-ulcerogenic activity of some plants used in folk medicine of Pinarbasi (Kayseri, Turkey). J Ethnopharmacol. 2005; 101(1-3): 313-318.
[8] Avci G, Kupeli E, Eryavuz A, Yesilada E, Kucukkurt I. Anti-hyper cholesterolaemic and antioxidant activity assessment of some plants used as remedy in Turkish folk medicine. J Ethnopharmacol. 2006; 107(3): 418-423.
[9] Tomovic MT, Cupara SM, Popovic-Milenkovic MT, Ljujic BT, Kostic MJ, Jankovic SM. Antioxidant and anti-inflammatory activity of Potentilla reptans L.  Acta Pol Pharm.2015; 72(1): 137-145.
[10] Rigaud J,  Escribano-Bailon MT,  Prieur C,  Souquet JM,  Cheynier V. Normal-phase  high-performance separation  of  procyanidins  from grape  seeds. J Chromatogr A. 1993; 654(2): 255-260.
[11] Li JY, Li Y, Gong HY, Zhao XB, Li LZ.  Protective effects of n-butanol extract of Potentilla anserina on acute myocardial ischemic injury in mice. Zhong Xi Yi Jie He Xue Bao. 2009; 7(1): 48-52.
[12] Qin X, Lv Q, Zhang X, Chen F, Li L, Zhang Y. Study on protective effect of alcohol extract of Potentilla anserinea against acute myocardial ischemia/reperfusion-induced myocardial apoptosis in rats. Zhongguo Zhong Yao Za Zhi. 2012; 37(9): 1279-1284.
[13] Jankowski MHF, Kawas SA, Mukaddam-Daher S, Hoffman G, McCann SM, Gutkowska J.   Rat heart: a site of oxytocin production and action. Proc Natl Acad Sci USA. 1998; 95(24): 14558-14563.
[14] Hausenloy DJ. Signalling pathways in ischaemic postconditioning. Thromb Haemost. 2009; 101(4): 626-634.
[15] Rubino A, Yellon DM. Ischaemic preconditioning of the vasculature: an overlooked phenomenon for protecting the heart? Trends Pharmacol Sci. 2000; 21(6): 225-230.
[16] Tomczyk M, Pleszczyńska M, Adrian Wiater A. Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity. Molecules. 2010; 15(7): 4639-4651.
[17] Yassa N, Masoomi F, Rohani Rankouhi SE, Hadjiakhoondi A. Chemical composition and antioxidant activity of the extract and essential oil of Rosa damascena from Iran, population of Guilan. Daru J Pharm Sci. 2009; 17(3): 175-180.
[18] Mehdipour S, Yasa N, Dehghan Gh, Khorasani R, Mohammadirad A, Rahimi R, Abdollahi M. Antioxidant potentials of Iranian Carica papaya juice in vitro and in vivo are comparable to α-tocopherol. Phytother Res. 2006; 20(7): 591-594.
[19] Evans WC, Evans D, Trease GE. Pharmacopoeial and related drugs of biological origin (part 6), phenols and phenolic glycosides. In: Trease and Evans' pharmacognosy. 15th ed. Edinburgh: WB Saunders, 2002.
[20] Zengin G, Menghini L, Malatesta L, De Luca E, Bellagamba G, Uysal S, Aktumsek A, Locatelli M. Comparative study of biological activities and multicomponent pattern of two wild Turkish species: Asphodeline anatolica and Potentilla speciosa. J Enzyme Inhib Med Chem. 2016; 31(S1): 203-208.
[21] Polshekan M, Jamialahmadi K, Khori V, Alizadeh AM, Saeidi M, Ghayour-Mobarhan M, Jand Y, Ghahremani MH, Yazdani Y. RISK pathway is involved in oxytocin post conditioning in isolated rat heart. Peptides. 2016; 86(1): 55-62.
[22] Curtis MJ, Walker MJ. Quantification of arrhythmias using scoring systems: an examination of seven scores in an in vivo model of regional myocardial ischaemia. Cardiovasc Res. 1988; 22(9): 656-665.
[23] Li J, Li L, Gong H, Li Z, Zhang X. Effect of Potentilla anserina L. on cardiomyocyte apoptosis induced by hypoxia. Chin J Geriatr Heart Brain Vessel Dis. 2007; 9(9): 619-622.
[24] Li JY, Li LZ, Zhang YL, Zhang XN, Gong HY. Protective effect of Poteutilla anserina L. on hypoxia injury in neonatal rats cardiomyocytes. Chin J New Drugs. 2007; 16(12): 944-946.
[25] Lv Q, Qin XJ, Zhang XN, Li LZ, Chen FQ, Guo P. Effects of Poteutilla anserina L. on acute ischemic myocardiac muscle and the differentially expressed proteins in serum in rats. Acta Academiae Medicinae CPAF. 2011; 20(6): 422-429.
[26] Shen CC, Chang YS, Ho LK. Nuclear magnetic resonance studies of 5,7-dihydroxyflavonoids. Phytochemistry. 1993; 34(3): 843-845.
[27] Heusch G, Boengler K, Schulz R. Cardioprotection: nitric oxide, protein kinases and mitochondria. Circulation. 2008; 118(19): 1915-1919.
[28] Schulz RCM, Behrends M, Downey JM, Heusch G. Signal transduction of ischemic preconditioning. Cardiovasc Res. 2001; 52(2): 181-198.
[29] Ahlenstiel T, Burkhardt G, Köhler H, Kuhlmann MK. Bioflavonoids attenuate renal proximal tubular cell injury during cold preservation in Euro-Collins and University of Wisconsin solutions. Kidney Int. 2003; 63(2): 554-563.
[30] Polshekan M, Khori V, Alizadeh AM, Ghayour-Mobarhan M, Saeidi M, Jand Y, Rajaei M, Farnoosh GR, Jamialahmadi KH. The SAFE pathway is involved in the postconditioning mechanism of oxytocin in isolated rat heart. Peptides. In press.
[31] Kruger MJ, Davies N, Myburgh KH, Lecour S. Proanthocyanidins, anthocyanins and cardiovascular diseases. Food Res Int. 2014; 59(1): 41-52.
[32] Mattera R, Benvenuto M, Giganti MG, Tresoldi I, Pluchinotta FR, Bergante S, Tettamanti G, Masuelli L, Manzari V, Modesti A, Bei R. Effects of polyphenols on oxidative stress-mediated injury in cardiomyocytes. Nutrients. 2017; 9(5): 3-43.
[33] He H, Xu J, Xu Y, Zhang C, Wang H, He Y, Wang T, Yuan D. Cardioprotective effects of saponins from Panax japonicus on acute myocardial ischemia against oxidative stress-triggered damage and cardiac cell death in rats. J Ethnopharmacol. 2012; 140(1): 73-82.
[34] Uysal S, Zengin G, Locatelli M, Bahadori MB, Mocan A, Bellagamba G, De Luca E, Mollica A, Aktumsek A. Cytotoxic and enzyme inhibitory potential of two Potentilla species (P. speciosa L. and P. reptans Willd.) and their chemical composition. Front Pharmacol. 2017; 8: 1-11.
[35] Wallerath T, Li H. Godtel-Ambrust U, Schwarz PM, Forstermann U. A blend of polyphenolic compounds explains the stimulatory effect of red wine on human endothelial NO synthase. Nitric Oxide. 2005; 12(2): 97-104.
[36] Wallerath T, Deckert G, Ternes T, Anderson H, Li H, Witte K, Forstermann U. Resveratrol, a polyphenolic phytoalexin present in red wine, enhances expression and activity of endothelial nitric oxide synthase. Circulation. 2002; 106(13): 1652-1658.
[37] Naseem SA, Kontos MC, Rao PS, Jesse RL, Hess ML, Kukreja RC. Sustained inhibition of nitric oxide by NG-nitro-L-arginine improves myocardial function following ischemia/reperfusion in isolated perfused rat heart. J Mol Cell Cardiol. 1995; 27(1): 419-426.
[38] Khori V, Davarian A, Nayebpour M, Saleki S, Salehi A, Shirafkan A, Badaghabadi F, Pourabouk M, Alizadeh A, Changiz S. Effect of nitric oxide modulation on the basic and rate-dependent electrophysiological properties of AV-node in the isolated heart of rabbit: the role of adrenergic and cholinergic receptors. Physiol Pharmacol. 2010; 14(1): 12-22. 
Research Journal of Pharmacognosy
Volume 6, Issue 1
January 2019
Pages 19-27

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