Anti-Inflammatory Effect and Skin Toxicity of Aqueous Extract of Dorema ammoniacum Gum in Experimental Animals

Document Type : Original paper


1 Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University (IAUPS), Tehran, Iran.

2 Regenerative Medicine Research Center (RMRC), Kermanshah University of Medical Sciences, Kermanshah, Iran. Pharmacceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

3 Pharmacceutical Sciences Research Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran. Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.


Background and objectives: Dorema ammoniacum gum resin is used in Iranian traditional medicine for different indications including inflammatory diseases which are on the rise. Considering the important role of inflammation in different diseases, in the present study, we aimed to investigate the in vivo anti-inflammatory activity and skin toxicity of Dorema ammoniacum gum extract (DAGE) in rats and rabbits. Methods: The systemic anti-inflammatory effect ofDAGE (100, 200 and 300 mg/kg, i.p.) was assessed by carrageenan-induced paw edema method in 30 min, 1, 2, 3 and 4 h after the carrageenan injection to thirty male Wistar rats divided into five groups of six each. Control and standard groups received the vehicle and mefenamic acid (30 mg/kg, i.p.), respectively. To assess the topical anti-inflammatory effect ofthegum, eighteen rats were divided into three groups of six: standard, vehicle, and test groups which received topical diclofenac gel, distilled water and DAGE, respectively. The acute dermal toxicity of DAGEwas evaluated in nine white New Zealand rabbits. Results: The results showed significant anti-inflammatory effects of DAGE in systemic treatment.The findings also indicated that all doses of DAGE were more potent than mefenamic acid. However, the topical anti-inflammatory activity of DAGE (100 mg/kg) was comparable to that of diclofenac gel 2% and showed no skin toxicity. Conclusion: The results of the present study suggest that DAGE has significant anti-inflammatory effects without any erythema and edema in topical use. These effects might be partially or wholly due to possible inhibition of or interference with the production of some inflammatory mediators, especially prostaglandins, histamine, serotonin, and bradykinin.


Main Subjects

[1] Bakhtiarian A, Shojaii A, Hashemi S, Nikoui V. Evaluation of analgesic and anti-inflammatory activity of Dorema ammoniacum gum in animal model. Int J Pharm Sci Res. 2017; 8(7): 3102-3106.
[2] Mozaffarian V. A dictionary of Iranian plant names. Tehran: Farhang-e-Mo'aser, 1996.
[3] Yousefzadi M, Mirjalili HM, Alnajar N, Zeinali A, Parsa M. Composition and in vitro antimicrobial activity of the essential oil of Dorema ammoniacum D. Don. fruit from Iran. J Serb Chem Soc. 2011; 76(6): 857-863.
[4] Motevalian M, Mehrzadi S, Ahadi S, Shojaii A. Anticonvulsant activity of Dorema ammoniacum gum: evidence for the involvement of benzodiazepines and opioid receptors. Res Pharm Sci. 2017; 12(1): 53-59.
[5] Gholami BA, Faravani M. The possibility of crop cultivation and utilization of edible gum from herb (Dorema ammoniacum D. Don) in dryland farming. J Agric Sci. 2015; 60(3): 369-380.
[6] Irvani N, Solouki M, Omidi M, Saidi A, Zare A. Seed germination and dormancy breaking in Dorema ammoniacum D., an endangered medicinal plant. Trakia J Sci. 2012; 10(1): 9-15.
[7] Irvani N, Solouki M, Omidi M, Zare A, Shahnazi S. Callus induction and plant regeneration in Dorem ammoniacum D., an endangered medicinal plant. Plant Cell Tiss Org Cult. 2010; 100(3): 293-299.
[8] Hosseini SAR, Naseri H, Azarnivand H, Jafari M, Rowshan V, Panahian AR. Comparing stem and seed essential oil in Dorema ammoniacum D. Don. from Iran. J Essent Oil Bear Pl. 2014; 17(6): 1287-1292.
[9] Iranshahi M, Shaki F, Mashlab A, Porzel A, Wessjohann LA. Kopetdaghins A-E, sesquiterpene derivatives from the aerial parts and the roots of Dorema kopetdaghense. J Nat Prod. 2007; 70(8): 1240-1243.
[10] Fullerton JN, Gilroy DW. Resolution of inflammation: a new therapeutic frontier. Nat Rev Drug Discov. 2016; 15(8): 551-567.
[11] Pountos I, Georgouli T, Bird H, Giannoudis P. Nonsteroidal anti-inflammatory drugs: prostaglandins, indications, and side effects. J Interferon Cytokine Res. 2011; 3(1): 19-27.
[12] Al-Omari B, Sim J, Croft P, Frisher M. Patient preferences for the pharmacological treatment of osteoarthritis: a feasibility study using adaptive choice-based conjoint analysis (acbca). Eur J Pers Cent Healthc. 2015; 3(2): 253-260.
[13] Adhami HR, Lutz J, Kählig H, Zehl M, Krenn L. Compounds from gum ammoniacum with acetylcholinesterase inhibitory activity. Sci Pharm. 2013; 81(3): 793-806.
[14] Jahandar F, Asgarpanah J, Najafizadeh P, Mousavi Z. Anti-inflammatory activity and chemical composition of Pycnocycla bashagardiana fruit’s essential oil in animal models. Iranian J Basic Med Sci. 2018; 21(2): 188-193.
[15] Morris CJ. Carrageenan-induced paw edema in the rat and mouse. Methods Mol Biol. 2003; 225:115-121.  
[16] Draize JH, Woodard G, Calvery HO. Methods for the study of irritation and toxicity of substances applied topically to the skin and mucous membranes. J Pharmacol Exp Ther. 1944; 82(3): 377-390.
[17] Fan AM, Kizer KW. Selenium. Nutritional, toxicologic, and clinical aspects. West J Med. 1990; 153(2): 160-167.
[18] Perianayagam  JB, Sharma SK,  Pillai KK. Antiinflammatory activity of Trichodesma indicum root extract in experimental animals. J Ethnopharmacol. 2006; 104(3): 410-414.
[19] Silva GN, Martins FR, Matheus ME. Investigation of anti-inflammatory and antinociceptive activities of Lantana trifolia. J Ethnopharmacol. 2005; 100(3): 254-259.  
[20] Sawadogo WR, Boly R, Lompo M,  Some N. Anti-inflammatory, analgesic and antipyretic activities of Dicliptera verticillata. Int J Pharmacol. 2006; 2(4): 435-438.
[21] Furst D, Manning D. Future directions in pain management. Clin Exp Rheumatol. 2001; 19(6): 71-76.
[22] Delnavazi M, Tavakoli S, Rustaie A, Batooli H, Yassa N. Antioxidant and antibacterial activities of the essential oils and extracts of Dorema ammoniacum roots and aerial parts. Res J Pharmacogn. 2014; 1(4): 11-18. 
[23] Rajani M, Saxena N, Ravishankara M, Desai N, Padh H. Evaluation of the antimicrobial activity of ammoniacum gum from Dorema ammoniacum. Pharm Biol. 2002; 40(7): 534-541.
[24] Adhami HR, Lutz J, Kählig H, Zehl M, Krenn L. Compounds from gum ammoniacum with acetylcholinesterase adhesives combination. Daru J Pharm Sci. 2013; 21(1): 1-14.
[25] Yousefzadi M, Heidari M, Akbarpour M, Mirjalili MH, Zeinali A, Parsa M. In vitro cytotoxic activity of the essential oil of Dorema ammoniacum D. Don. Middle-East J Sci Res. 2011; 7(4): 511-514.
[26] Lee SE, Choi Y, Kim SE, Noh EB, Kim SC. Differential effects of topical corticosteroid and calcineurin inhibitor on the epidermal tight junction. Exp Dermatol. 2013; 22(1): 59-61.
[27] Panchaxari DM, Pampana S, Pal T, Devabhaktuni B, Aravapalli AK. Design and characterization of diclofenac diethylamine transdermal patch using silicone and acrylic
inhibitory activity. Sci Pharm. 2013; 81(3): 793-806.
[28] Marto J, Baltazar D, Duarte A, Fernandes A, Gouveia L, Militão M, Salgado A, Simões S, Oliveira E, Ribeiro HM. Topical gels of etofenamate: in vitro and in vivo evaluation. Pharm Dev Technol. 2015; 20(6): 710-715.
[29] Lau WM, White AW, Gallagher S, Donaldson M, McNaughton G, Heard CM. Scope and limitations of the co-drug approach to topical drug delivery. Curr Pharm Des. 2008; 14(8): 794-802.