Isolation and characterization of phytochemicals of Johrenia paucijuga (DC.) Bornm.

Document Type : Original paper


1 Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

2 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.


Background and objectives: The genus Johrenia belongs to Umbelliferae family and contains five species that are endemic to Iran. Johrenia paucijuga grows widely in the north-west, west and center of Iran. So far, there has been no research about phytochemistry of J. paucijuga. In the present study, phytochemicals of the plants have been isolated and their structures have been elucidated. Methods: The aerial parts were dried and cut into small pieces, then extracted with ethyl acetate and methanol using perculator apparatus at room temperature. The methanol extract was extracted again with, petroleum ether and butanol. The separation and isolation process was carried out using column (silica gel and Sephadex LH-20) and thin layer chromatographic (TLC) methods. Structure elucidation of the purified compounds were based on ¹H and ¹³C-NMR data, in comparison with those reported in the previous literatures. Results: The isolated compounds from the ethyl acetate and butanol extracts of J. paucijuga were identified as β-sitosterol, β- stigmasterol and quercetin. Quercetin is a bioactive flavonoid widely used as a health supplement. β- sitosterol and β-stigmasterol are phytosteroles (plants sterols) with chemical structure similar to that of cholesterol and are sometimes used in treating hypercholesterolemia. Conclusion: Regarding the valuable biological properties of the isolated compounds, different biological effects could be expected from the plant.


[1] Cragg GM, Newman DJ. Plants as a source of anti-cancer agents. J Ethnopharmacol. 2005; 100(1): 72-79.
[2] Hadavand Mirzaei H, Faraji R, Hooshidari F, Bigdelo M, Rezaei K. Evaluation of phytochemical composition of Opsicarpium insignis Mozaff. From Iran by nano scale injection techniques. Int J Chem Tech Res. 2013; 5(4): 1911-1914.
[3] Dogan B, Duran A, Bagci Y, Dinc M, Martin E, Cetin O, Ozturk M. Phylogenetic relationships among the taxa of the genus Johrenia DC.(Apiaceae) from Turkey based on molecular method. Bangladesh J Plant Taxon. 2010; 17(2): 113-120.
[4] Gohari AR, Saeidnia S, Shahverdi AR, Yassa N, Malmir M, Mollazade K, Naghinejad AR. Phytochemistry and antimicrobial compounds of Hymenocrater calycinus. Eurasian Asia J Bio Sci. 2009; 3: 64-68.
[5] Ahmed Y, Rahman S, Akhtar P, Islam F, Rahman M, Yaakok Z. Isolation of steroids from n-hexane extract of the leaves of Saurauia roxburghii. Int Food Res J. 2013; 20(5): 2939-2943.
[6] Kyriakou E, Primikyri A, Charisiadis P, Katsoura M, Gerothanassis IP, Stamatis H, Tzakos AG. Unexpected enzyme-catalyzed regioselective acylation of flavonoid aglycones and rapid product screening. Org Biomol Chem. 2012; 10(1): 1739-1742.
[7] Matsuoka K, Nakazawa T, Nakamura A, Honda C, Endo K, Tsukada M. Study of thermodynamic parameters for solubilization of plant sterol and stanol in bile salt micelles. Chem Phys Lipids. 2008; 154(2): 87-93.
[8] Awad AB, Fink CS. Phytosterols as anticancer dietary components: evidence and mechanism of action. J Nutr. 2000; 130(9): 2127-2130.
[9] Meana MCH, Patni V. Isolation and identification of flavonoid quercetin from Citrullus colocynthis (Linn.) Schrad. Asian J Exp Sci. 2008; 22(1): 137-142.
[10] Muhit MdA, Tareq SM, Apu AS, Basak D, Islam MS. Isolation and identification of compounds from the leaf extract of Dillenia indica Linn. Bangladesh Pharm J. 2010; 13(1): 220-225.
[11] Koraganco NV, Kashka ZHN, Borisov EV.¹³C-NMR spectra of functionally substituted 3β-chloroderivation of cholesterol and β-sitosterol. Chem Nat Compd. 2000; 36(6): 395-398.
[12] Chaturvedula VS, Prakash I. Isolation of stigmasterol and β-sitosterol from the dichloromethane extract of Rubus suavissimus. Int Cur Pharm J. 2012; 1(9): 239-242.
[13] Markham KR. Techniques of flavonoid identification. New York: Academic press, 1982.
[14] Habibi Z, Monfared A, Masoudi Sh, Rustaiyan A. Essential oil of Johrenia ramosissima Mozaffarian from Iran. J Essent Oil Res. 2004; 16(5): 395-396.