Effects of Berberis vulgaris fractions on PTZ Induced seizure in male rats


1 Evidence-based Phytotherapy & Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran. Department of Pharmacognosy, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran.

2 Department of Physiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.

3 Student Research Committee, Paramedical School, Alborz University of Medical Sciences, Karaj, Iran.

4 Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.

5 Research Center for Social Determination of Health, Alborz University of Medical Sciences, Karaj, Iran.


Background and objectives: Berberis vulgaris L (Berberidaceae) is a medicinal plant that is distributed in different parts of Iran; it is grown as a wild or cultivated plant. It has different pharmacological activities such as antioxidant, anti-inflammatory, anti-arrhythmic, sedative and anti-malaria effects. In this study, the anti-seizure activity of different fractions of this plant was evaluated. Methods: Seventy two rats were randomly divided in to nine groups (n=8 in each group). (1): negative control group (normal saline 10mL/kg), (2): positive control group (sodium valproate 1 mg/kg), (3, 4, 5): hydroalcoholic extract-treated groups (100, 200, 400 mg/kg), (6, 7): methanol fraction-treated groups (100 and 200 mg/kg) and (8, 9): chloroform fraction-treated group (100 and 200 mg/kg). Thirty minute after peritoneal injection of different doses of extract, fractions, saline and gavage of sodium valproate, PTZ (45 mg/kg) was injected and they were immediately transferred to a special cage, and the seizure parameters were evaluated for 30 min. Result: The injection of different doses of hydroalcoholic extract and different fractions had a dose-dependent effect on prolongation of latency to the onset of seizures. The effective dose was 400 mg/kg of hydroalcoholic extract and 200 mg/kg of methanol fraction. They decreased the rate of mortality and the number of suddenly seizures jumping significantly. Conclusion: The present study demonstrated that the hydroalcoholic extract and methanol fraction of B. vulgaris showed anticonvulsant activity in PTZ-induced seizures in mice. Therefore, this plant may be more useful in petit mal epilepsy.