In vitro anti-biofilm activity of Quercus brantii subsp. persica on human pathogenic bacteria

Document Type : Original paper


1 Rab-e -Rashidi University, Tabriz, Iran.

2 Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Department of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.


Background and objectives: Quercus brantii subsp. persica is used in folk medicine to treat infections in Iran. There is not available report on the anti-biofilm activity of Quercus brantii subsp.  persica. The aim of the present study was to investigate the effects of Quercus brantii subsp. persica against bacterial biofilms. Methods: Eighty biofilm producing strains of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa were collected. Quercus brantii subsp. persica fruits aqueous extraction (QBAE) was prepared though maceration method. Chemical analysis to distinguish the main components of the QBAE was carried out using thin-layer chromatography. The antibacterial effects of QBAE on bacterial isolates were determined by the Kirby-Bauer and broth microdilution methods. The antibiofilm effects of QBAE on bacterial isolates were determined using a microtiter assay. Results: The Quercus brantii subsp. persica exhibited bacterial growth inhibition and bactericidal activity on the majority of the strains at concentrations between 0.2 and 1.2 mg/mL. The average of biofilm formation inhibition by Quercus brantii subsp. persica at a minimum inhibitory concentration MIC50 in Pseudomonas aeruginosa, Escherichia coli, Staphylococcus epidermidis and Staphylococcus aureus strains were 35%, 45%, 57% and 61%, respectively. coumarins, phenols, terpenes and steroids were found in the QBAE by TLC. Conclusion: The results showed that Quercus brantii subsp. persica aqueous extraction was effective against the tested microorganisms and showed anti-biofilm activity which can be a basis for future studies to investigate for new anti-biofilm drugs.


[1] Southey-Pillig CJ, Davies DG, Sauer K. Characterization of temporal protein production in Pseudomonas aeruginosa biofilms. J Bacteriol. 2005; 187(23): 8114-8126.
[2] Stewart PS, Costerton JW. Antibiotic resistance of bacteria in biofilms. Lancet. 2001; 358(9276): 135-138.
[3] Ghotaslou R, Salahi B. Effects of oxygen on in vitro biofilm formation and antimicrobial resistance of Pseudomonas aeruginosae. Pharm Sci. 2013; 19(3): 96-99.
[4] Sadeghian I, Hassanshahi M, Sadeghian S, Jamali S. Antimicrobial effects of Quercus brantii fruits on bacterial pathogens. Jundishapur J Microbiol. 2012; 5(3): 465-469.
[5] Safary A, Motamedi H, Maleki S, Seyednejad IS. A preliminary study on the antibacterial activity of Quercus brantii against bacterial pathogens, particularly enteric pathogens. Int J Botany. 2009; 5(2): 176-180.
[6] Tahmouzi S. Optimization of polysaccharides from Zagros Oaklea fusing RSM: antioxidant and antimicrobial activities. Carbohydr Polym. 2014; 106: 238-246.
[7] Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M. Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis. 2011; 15(4):305-311.
[8] Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas, 2nd ed. Berlin: Springer-Verlag, 1996.
[9] Shan B, Cai YZ, Brooks JD, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int J Food Microbiol. 2007; 117(1): 112-119.
[10] Ulrey RK, Barksdale SM, Zhou W, van Hoek ML. Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa. BMC Complement Altern Med. 2014; 14(1): 499.
[11] Czaczyk K, Myszka K. Mechanisms determining bacterial biofilm resistance to antimicrobial factors. Biotechnologia. 2007; 74(1): 40-52.
[12] Taylor EN, Webster TJ. The use of superparamagnetic nanoparticles for prosthetic biofilm prevention. Int J Nanomedicine. 2009; 4: 145-152.
[13] Ferrazzano GF, Amato I, Ingenito A, De Natale A, Pollio A. Anti-cariogenic effects of polyphenols from plant stimulant beverages (cocoa, coffee, tea). Fitoterapia. 2009; 80(5): 255-262.
[14] Schito AM, Piatti G, Stauder M, Bisio A, Giacomelli E, Romussi G, Pruzzo C. Effects of demethylfruticuline A and fruticuline A from Salvia corrugata Vahl. on biofilm production in vitro by multiresistant strains of Staphylococcus aureus, Staphylococcus epidermidis and Enterococcus faecalis. Int J Antimicrob Agents. 2011; 37(2): 129-134.
[15] Sampaio FC, Pereira Mdo S, Dias CS, Costa VC, Conde NC, Buzalaf MA. In vitro antimicrobial activity of Caesalpinia ferrea Martius fruits against oral pathogens. J Ethnopharmacol. 2009; 124(2): 289-294.
[16] Da Trentin DS, Giordani RB, Zimmer KR, da Silva AG, da Silva MV, Correia MT, Baumvol IJ, Macedo AJ. Potential of medicinal plants from the Brazilian semi-arid region (Caatinga) against Staphylococcus epidermidis planktonic and biofilm lifestyles. J Ethnopharmacol. 2011; 137(1): 327- 335.
[17] Praud-Tabaries A, Dombrowsky L, Bottzek O, Briand J, Blache Y. Synthesis of a polyprenyl-type library containing 1,4-disubstituted-1,2,3-triazoles with anti-biofilm activities against Pseudoalteromonas sp. Tetrahedron Lett. 2009; 50(14): 1645-1648.
[18] Moghadam MS, Maleki S, Darabpour E, Motamedi H, Nejad SM. Antibacterial activity of eight Iranian plant extracts against methicillin and cefixime restistant Staphylococcous aureus strains. Asian Pac J Trop Med. 2010; 3(4): 262-265.