Assessing the Anti-Colitis Properties of Aqueous and Hydroalcoholic Extracts of Pinus eldarica in Rats with Acetic Acid-Induced Colitis

Document Type : Original paper

Authors

1 Department of Pharmacology and Pharmaceutical Sciences Research Centre, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

2 School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

4 Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

10.22127/rjp.2024.434773.2323

Abstract

Background and objectives: Ulcerative colitis is a challenging inflammatory bowel disease that requires new treatments. Pinus eldarica can be a suitable candidate for this disease due to its anti-inflammatory, anti-ulcerative and antioxidant properties. Methods: Pinus eladarica aqueous and hydroalcoholic extracts of barks were standardized according to the total phenols, flavonoids and proanthocyanidin contents. Three doses (100, 200, and 400 mg/kg, p.o.) of both extracts were separately administered to rats with acetic acid-induced colitis for a period of five days. Reference groups received dexamethasone (1 mg/kg, i.p.) or mesalazine (150 mg/kg, p.o.) while control groups were treated with normal saline. Results: Both extracts reduced the macroscopic parameters of colitis (weight of colon, ulcer area, ulcer severity and ulcer index) significantly compared with control groups, especially in lower doses (100, 200 mg/kg). Similarly, the extracts improved the microscopic parameters (severity and extent of inflammation, leukocyte infiltration, crypt damage, and total colitis score) except for the dose of 400 mg, which was not effective. The decrease in myeloperoxidase activity and malondeladehyde values ​​was also significant for both extracts at all doses. Conclusion: Pinus eldarica bark extracts are effective in treating and reducing the damage caused by colitis, although it is necessary to adjust the effective dosage. Lower doses of extracts, especially hydroalcoholic one showed better therapeutic effects. Further studies are necessary to identify effective compounds, particularly in the hydroalcoholic extract, for producing an herbal drug for the clinical setting.
 

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References

  • Guan Q. A comprehensive review and update on the pathogenesis of inflammatory bowel disease. J Immunol Res. 2019; Article ID 7247238.
  • Friedrich M, Pohin M, Powrie F. Cytokine networks in the pathophysiology of inflammatory bowel disease. Immunity. 2019; 50(4): 992-1006.
  • Terdiman JP, Steinbuch M, Blumentals WA, Ullman TA, Rubin DT. 5-Aminosalicylic acid therapy and the risk of colorectal cancer among patients with inflammatory bowel disease. Inflamm Bowel Dis. 2007; 13(4): 367-371.
  • Summers RW. Novel and future medical management of inflammatory bowel disease. Surg Clin North Am. 2007; 87(3): 727-741.
  • Selvamani S, Mehta V, El Enshasy HA, Thevarajoo S, El Adawi H, Zeini I, Pham K, Varzakas T, Abomoelak B. Efficacy of probiotics-based interventions as therapy for inflammatory bowel disease: a recent update. Saudi J Biol Sci. 2022; 29(5): 3546-3567.
  • Li C, Wang J, Ma R, Li L, Wu W, Cai D, Lu Q. Natural-derived alkaloids exhibit great potential in the treatment of ulcerative colitis. Pharmacol Res. 2022; Articl ID 105972.
  • Ribeiro D, Proenca C, Rocha S, Lima JL, Carvalho F, Fernandes E, Freitas M. Immunomodulatory effects of flavonoids in the prophylaxis and treatment of inflammatory bowel diseases: a comprehensive review. Curr Med Chem. 2018; 25(28): 3374-3412.
  • Caban M, Lewandowska U. Polyphenols and the potential mechanisms of their therapeutic benefits against inflammatory bowel diseases. J Funct Foods. 2022; Article ID 105181.
  • Ge J, Liu Z, Zhong Z, Wang L, Zhuo X, Li J, Xiaoying J, Xiang-Yang Y, Tian X, Renren B. Natural terpenoids with anti-inflammatory activities: Potential leads for anti-inflammatory drug discovery. Bioorg Chem. 2022; Article ID 105817.
  • Zargary A. Medicinal plants. 5th Tehran: Tehran University Press, 1996.
  • Mamedov N, Craker LE. Medicinal plants used for the treatment of bronchial asthma in Russia and Central Asia. J Herbs Spices Med Plants. 2001; 8(2-3): 91-117.
  • Mamedov N, Gardner Z, Craker LE. Medicinal plants used in Russia and Central Asia for the treatment of selected skin conditions. J Herbs Spices Med Plants. 2005; 11(1-2): 191-222.
  • Gulati OP. The nutraceutical pycnogenol: its role in cardiovascular health and blood glucose control. Biomed Rev. 2005; 16: 49-57.
  • Afsharypour S, Sanaty F. Essential oil constituents of leaves and fruits of Pinus eldarica J Essent oil Res. 2005; 17(3): 327-328.
  • Iravani S, Zolfaghari B. Phytochemical analysis of Pinus eldarica Res Pharm Sci. 2014; 9(4): 243-250.
  • Yesil‐Celiktas O, Ganzera M, Akgun I, Sevimli C, Korkmaz KS, Bedir E. Determination of polyphenolic constituents and biological activities of bark extracts from different Pinus J Sci Food Agric. 2009; 89(8): 1339-1345.
  • Kim NY, Jang MK, Lee DG, Yu KH, Jang H, Kim M, Kim SG, Yoo BH, Lee SH. Comparison of methods for proanthocyanidin extraction from pine (Pinus densiflora) needles and biological activities of the extracts. Nutr Res Pract. 2010; 4(1): 16-22.
  • Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin Ciocalteu reagent. Nat Protoc. 2007; 2(4): 875-877.
  • Karapandzova M, Stefkov G, Cvetkovikj I, Stanoeva JP, Stefova M, Kulevanova S. Flavonoids and other phenolic compounds in needles of Pinus peuce and other pine species from the Macedonian flora. Nat Prod Commun. 2015; 10(6): 987-990.
  • Porter LJ, Hrstich LN, Chan BG. The conversion of procyanidins and prodelphinidins to cyanidin and delphinidin. Phytochemistry. 1985; 25(1): 223-230.
  • Kayvanara AH, Yegdaneh A, Talebi A, Minaiyan M. Evaluating anti-Inflammatory effect of hydroalcoholic extracts of Citrus medica pulp and peel on rat model of acute colitis. Res J Pharmacogn. 2023; 10(2): 29-38.
  • Jalalipour M, Yegdaneh A, Talebi A, Minaiyan M. Salvia officinalis leaf extracts protect against acute colitis in rats. Res Pharm Sci. 2022; 17(4): 350-359.
  • Minaiyan M, Ghannadi A, Mahzouni P, Nabi-Meibodi M. Anti-ulcerogenic effect of ginger (rhizome of Zingiber officinale Roscoe) hydroalcoholic extract on acetic acid-induced acute colitis in rats. Res Pharm Sci. 2009; 3(2): 15-22.
  • Cooper HS, Murthy SN, Shah RS, Sedergran DJ. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest. 1993; 69(2): 238-249.
  • Motavallian-Naeini A, Minaiyan M, Rabbani M, Mahzuni P. Anti-inflammatory effect of ondansetron through 5-HT3 receptors on TNBS-induced colitis in rat. EXCLI J. 2012; 11: 30-44.
  • Vieito C, Fernandes É, Velho MV, Pires P. The effect of different solvents on extraction yield, total phenolic content and antioxidant activity of extracts from pine bark (Pinus pinaster atlantica). Chem Eng Trans. 2018; 64: 127-132.
  • Minaiyan M, Sajjadi SE, Naderi N, Taheri D. Anti-inflammatory effect of Kelussia odoratissima hydroalcoholic extract on acetic acid- induced acute colitis in rats. J Rep Pharm Sci. 2014; 3(1): 28-35.
  • Ghadirkhomi A, Safaeian L, Zolfaghari B, Agha Ghazvini MR, Rezaei P. Evaluation of acute and sub-acute toxicity of Pinus eldarica bark extract in Wistar rats. Avicenna J Phytomed. 2016; 6(5): 558-566.
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid peroxidation. Meth Enzymol. 1990; 186: 421-431.
  • Tian Y, Wang K, Wang Z, Li N, Ji G. Chemopreventive effect of dietary glutamine on colitis-associated colon tumorigenesis in mice. J Carcinog. 2013; 34(7): 1593-1600.
  • Villablanca EJ, Selin K, Hedin CRH. Mechanisms of mucosal healing: treating inflammatory bowel disease without immunosuppression. Nat Rev Gastroenterol Hepatol. 2022; 19(8): 493-507.
  • McQuaid KR. Drugs used in the treatment of gastrointestinal disease. In: Katzung BG, editor. Basic and clinical pharmacology. 11th New York: McGraw Hill Companies, 2007.
  • Huseini HF, Anvari MS, Khoob YT, Rabbani S, Sharifi F, Arzaghi SM, Fakhrzadeh H. Anti-hyperlipidemic and anti-atherosclerotic effects of Pinus eldarica nut in hypercholesterolemic rabbits. DARU J Pharm Sci. 2015; 23(1): 32-37.
  • Maritim A, Dene B, Sanders R, Watkins III J. Effects of pycnogenol treatment on oxidative stress in streptozotocin‐induced diabetic rats. J Biochem Mol Toxicol. 2003; 17(3): 193-199.
  • Piazza S, Fumagalli M, Martinelli G, Pozzoli C, Maranta N, Angarano M. Hydrolyzable tannins in the management of Th1, Th2 and Th17 inflammatory-related diseases. 2022; Article ID 27217593.
  • Kaundun SS, Fady B, Lebreton P. Genetic differences between Pinus halepensis, Pinus brutia and Pinus eldarica based on needle flavonoids. Biochem Syst Ecol. 1997; 25(6): 553-562.
  • O’Leary KA, de Pascual-Tereasa S, Needs PW, Bao YP, O’Brien NM, Williamson G. Effect of flavonoids and vitamin E on cyclooxygenase-2 (COX-2) transcription. Mutat Res. 2004; 551(1-2): 245-254.
  • Gupta M, Mishra V, Gulati M, Kapoor B, Kaur A, Gupta R, Tambuwala MM. Natural compounds as safe therapeutic options for ulcerative colitis. Inflammopharmacol. 2022; 30(2): 397-434.
  • Lyu YL, Zhou HF, Yang J, Wang FX, Sun F, Li JY. Biological activities underlying the therapeutic effect of quercetin on inflammatory bowel disease. Mediators Inflamm. 2022; Article ID 5665778.
  • Frankel EN, Meyer AS. The problems of using one‐dimensional methods to evaluate multifunctional food and biological antioxidants. J Sci Food Agric. 2000; 80(13): 1925-1941.
  • Basholli-Salihu M, Schuster R, Hajdari A, Mulla D, Viernstein H, Mustafa B, Mueller M. Phytochemical composition, anti-inflammatory activity and cytotoxic effects of essential oils from three Pinus Pharm Biol. 2017; 55(1): 1553-1560.

 

Research Journal of Pharmacognosy
Volume 11, Issue 2
April 2024
Pages 61-70

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