An Insight into the Traditional Uses, Phytoconstituents and Pharmacological Activities of the Genus Tylophora

Document Type : Review


1 Department of Pharmacognosy, Vishnu Institute of Pharmaceutical Education & Research, Narsapur, Medak Dist, Telangana, India.

2 Department of Pharmaceutics, Raghu College of Pharmacy, Visakhapatnam, Andhra Pradesh, India.

3 Department of Pharmaceutical Chemistry, Maharishi Arvind College of Pharmacy Jaipur, Rajasthan, India.


Traditional plants have huge demand as medicines to treat a wide range of illnesses. Tylophora is an important genus of medicinal plant in India, used to treat asthma and other ailments. The plants of this genus have been studied in vivo and in vitro for various pharmacological properties. In this article, we have given information regarding ethnomedicinal importance, phytochemistry and pharmacological uses of 18 species of Tylophora. Comprehensive information regarding different species of Tylophora were collected using different keywords in various electronic databases such as ACS, Google Scholar, PubMed, Science Direct, SciFinder, Web of Science, Springer Link, library search, J gate, Wiley, Semantic Scholar and ResearchGate since 1960 to 2023. Additionally, data was collected from some textbooks and chapters like Flora of India and Indian medicinal plants. This article highlights the traditional uses, phytochemistry, and pharmacological activities of the few studied taxa of Tylophora that would serve as a reference for pharmaceutical research. More than 100 compounds have been isolated from selected species of the genus Tylophora. Among them, phenanthroindolizidine alkaloids have received the most attention and are the most abundant active constituents of the plant. Other types of active components of genus Tylophora include C21 glycosides, secoiridoids, triterpenes, and furano alkaloids. These compounds have shown a variety of therapeutic activities like antiasthmatic, antitumour, antimicrobial, antidiabetic and antiallergic properties. This review can be an important scientific resource for further research.


Main Subjects

  • Tseng YH, Chao CT. Tylophora lui (Apocynaceae), a new species from Taiwan. Ann Bot Fenn. 2011; 48(6): 515–518.
  • Forster PI. A taxonomic revision of TylophoraBr. (Asclepidaceae: Marsdenieae) in Australia. Aust Syst Bot. 1992; 5(1): 29–51.
  • Mary EE, Peter VB. A revised classification of the Apocyanaceaes.l. Bot Rev. 2000; 66(1): 1–56.
  • Jagtap AP, Singh NP. Fascicles of flora of India fascicle 24. Calcutta: Botanical Survey of India, 1999.
  • Ritika G, Saraswati P, Neetu Y, Jaya D, Sarvesh P, Swapnil S. Tylophora indica (Burm. f.) merr: an insight into phytochemistry and pharmacology. J Ethnopharmacol. 2020; 262: 1–18.
  • Nazar S, Hussain MA, Khan A, Muhammad G, Bukhari SNA. Alkaloid-rich plant Tylophora indica current trends in isolation strategies, chemical profiling and medicinal applications. Arab J Chem. 2020; 13(8): 6348–6365.
  • Malathi R, Patric G. Evaluation of preliminary toxicity studies on methanolic leaves of Tylophora asthmatica in experimental rats. J Pharmacol Toxicol. 2008; 3(1): 34–40.
  • Rajendran S, Jeyalatchagan S, Jayaraj K, Singamoorthy A, Muniappan A. Traditional herbal medicines practiced by the ethnic people in Sathyamangalam forests of Western Ghats, India. Eur J Integr Med. 2017; 16: 61–72.
  • Phillipson JD, Tezcan I, Hylands PJ. Alkaloids of Tylophora species from Sri Lanka. Planta Med. 1974; 25(4): 301–309.
  • Bhavya J, Vineetha MS, Veena SM, Farhan Z, Uday M, Sunil SM, Govindappa M. Ethnomedicinal plants and isolated compounds against snake venom activity: a review. Indian J Nat Prod Resour. 2021; 12(4): 491–505.
  • Cheng-Yu C, Guo-Yuan Z, Jing-Rong W, Zhi-Hong J. Phenanthroindolizidine alkaloids from Tylophora atrofolliculata with hypoxia-inducible factor-1 (HIF-1) inhibitory activity. RSC Adv. 2016; 6(83): 79958–79967.
  • Hostettmann K, Chinyanganya F, Maillard M, Wolfender JL. Chemistry, biological and pharmacological properties of African medicinal plants. Zimbabwe: University of Zimbabwe publications,
  • Prathamesh K, Saif K. Ethnomedicinal plants used in the treatment of respiratory diseases around Western Ghats region of Maharashtra, India. J Global Biosci. 2021; 10(3): 8447–8457.
  • Masur UA, Ferrao DM, Naik DD, Rodrigues MJ, Rodrigues VS. Phytochemical studies of Tylophora species of Goa. J Nat Prod. 2015; 8: 100–105.
  • Bhakshu LM, Venkata Ratnam K, Venkata Raju RR. Anticandidal activity and phytochemical analysis of certain medicinal plants from Eastern Ghats, India. Indian J Nat Prod Resour. 2016; 7(1): 25–31.
  • Bashir A, Niaz A, Saziq A, Shumila B. Blood pressure lowering effect of Tylophora hirsute Afr J Biotechnol. 2012; 11(12): 3009–3013.
  • Yue-Zhi L, Chun-Wei H, Chenh-Wei Y, Hsing-Yu H, Iou-Jun K, Yu-Sheng C, Ih-Sheng C, Hwan-You C, Shiou-Ju L. Isolation and biological activities of phenanthroindolizidine and septicine alkaloids from the Formosan Tylophora ovata. Planta Med. 2011; 77(17): 1932–1938.
  • Thangarajan S, Poochi SP, Basanth Kumar AT, Velliyur KG. Screening and GC-MS profiling of ethanolic extract of Tylophora pauciflora. Bioinformation. 2019; 15(6): 425–429.
  • Sheng Y, Chun-Ping T, Yang Y. Secoiridoids and xanthones from Tylophora secamonoides J Asian Nat Prod Res. 2008; 10(5): 591–596.
  • Pate MB, Rowson JM. Investigations of certain Nigerian medicinal plants, Part I. Preliminary pharmacological and phytochemical screenings for cardiac activity. Planta Med. 1964; 12(1): 33–42.
  • John NG, Marilyn JH, David GM, Jacob LP. Antiallergic activity of tylogenin, a novel steroidal compound from Tylophora sylvatica. Int J lmmunopharmacol. 1994; 16(8): 641–650.
  • Ruyani A, Sinta BD, Emilia, Zulfikar, Anansyah F, Putri SR, Sundaryono A. Preliminary studies on therapeutic effect of ethanolic extract of Tylophora villosa leaves against paracetamol-induced hepatotoxicity in mice. J Tradit Complement Med. 2018; 9(4): 285–
  • Lin YP, Fang QL, Xue YM, Fu SN, Hu CY, Huang F, Wang MM, Qiao X, Yin XQ, Zeng YC, Du CH, Zhao XJ, Li XP, Hua Y. Effects of Tylophora yunnanensis Schltr on regulating the gut microbiota and its metabolites in non-alcoholic steatohepatitis rats by inhibiting the activation of NOD-like receptor protein 3. J Ethnopharmacol. 2023; Article ID 116145.
  • Mulchandani NB. Isolation, biosynthesis and biological activity of alkaloids of Tylophora asthmatica, a versatile medicinal plant. Bombay: Bhabha Atomic Research Centre, 1987.
  • Reimche I, Haiqian YU, Ariantari NP, Liu Z, Merkens K, Rotfuß S, Peter K, Jungwirth U, Bauer N, Kiefer F, Jorg Martin N, Hans Gunther S, Peter P, Nicole T. Phenanthroindolizidine alkaloids isolated from Tylophora ovata as potent inhibitors of inflammation, spheroid growth, and invasion of triple-negative breast cancer. Int J Mol Sci. 2022; 23(18): 1–32.
  • Ehab MM, Hamdoon AM, Arafa M, Mohamed AA, Mohammad MA, Suliman AA, Mohammed MG, Hesham AMG, Fatema ESAR, Khaled S, Samy S, Riaz AK. In vitro anti-proliferative, and kinase inhibitory activity of phenanthroindolizidine alkaloids isolated from Tylophora indica. Plants (Basel). 2022; 11(10): 1–19.
  • Wadhawan VK, Sikka SK, Mulchandani NB. Minor alkaloids of Tylophora asthmatica X-ray analysis of tylophorinidine. Tetrahedron Lett. 1973; 14(51): 5091–5094.
  • Mulchandani NB, Venkatachalam SR. Tylophorinicine: a phenanthroindolizidine alkaloid from Tylophora asthmatica and Pergularia pallida. Phytochemistry. 1984; 23(5): 1206.
  • Xuehi H, Song G, Lihua F, Shishan Y, Xiaotian L. Cytotoxic alkaloids from the roots of Tylophora atrofolliculata. Planta Med. 2004; 70(5): 441–445.
  • Lijie C, Zeper A, Min X, Liyan Z, Song G, Wenyi H, Yun X, Feng L, Shisan Y. On-line identification of phenanthroindolizidine alkaloids in a crude extract from Tylophora atrofolliculataby liquid chromatography combined with tandem mass spectrometry. Rapid Commun Mass Spectrom. 2004; 18(2): 184–190.
  • Cheng-Yu C, Li-Ping B, Zhuo-Feng K, Yan L, Jing-Rong W, Zhi-Hong J. G-Quadruplex DNA-binding quaternary alkaloidsfrom Tylophora atrofolliculata. RSC Adv. 2016; 6(115): 114135–114142.
  • Zhenjia L, Haining L, Hongyan L, Yi Z, Haijing Z, Fuqin S, Song X, Yong L, Yikang S, Shishan Y, Xiaoguang C. Interaction studies of an anticancer alkaloid, (+)-(13as)-deoxytylophorinine, with calf thymus DNA and four repeated double-helical DNAs. 2011; 57(4): 310–320.
  • Xue SH, Shi SY, Xiao TL. Two phenanthroindolizidine alkaloids from Tylophora atrofolliculata. Chin Chem Lett. 2002; 13(1): 61–64.
  • Gellertt E, Govindachari R, Lakshmikantham V, Ragade IS, Rudzatsa R, Viswanathan N. The alkaloids of Tylophora crebriflora: structure and synthesis of tylocrebrine, a new phenanthroindolixidine alkaloid. Blake Proc Roy Soc Queensl. 1948; 59(168): 1008–1014.
  • Koppaka VR, Richard W, Bernice C. Alkaloids of Tylophora I: Isolationof six new alkaloids. J Pharm Sci. 1970; 59(10): 1501–1502.
  • Koppaka VR, Richard AW, Bernice C. Alkaloids of Tylophora 111: new alkaloids of Tylophora indica (Burm) Merrill and Tylophora dalzellii f. J Pharm Sci. 1971; 60(11): 1725–1726.
  • Bhutani KK, Mohammed A, Atal CK. 13a-Hydroxytylophorine from Tylophora hirsuta. 1985; 24(11): 2778–2780.
  • Mohammed A, Bhutani KK. Minor alkaloids of Tylophora hirsuta. Phytochemistry. 1987; 26(7): 2089–2092.
  • Bhutani KK, Mohammed A, Atal CK. Alkaloids from Tylophora hirsuta. Phytochemistry. 1984; 23(8): 1765–1769.
  • Viswanathan N. Alkaloids of Tylophora mollissima. J Nat Prod. 1985; 48(6): 997–998.
  • Zhen YY, Huang XS, Yu DQ, Yu SS. Antitumor alkaloids isolated from Tylophora ovata. Acta Bot Sin. 2002; 44(3): 349–353.
  • Zhen-Jia L, Hai-Ning L, Hong-Yan L, Yi Z, Hai-Jing Z, Fu-Qin S, Yi-Kang Si, Shi-Shan Y, Xiao-Guang C. Anticancer effect and neurotoxicity of S-(+)-deoxytylophorinidine, a new phenanthroindolizidine alkaloid that interacts with nucleic acids. J Asian Nat Prod Res. 2011; 13(5): 400–408.
  • Geun-Mook P, Jin-Kyung K. Septicine inhibits the production of inflammatory mediators in lipopolysaccharide-stimulated murine macrophages. J Life Sci. 2011; 21(9): 1310–1314.
  • Nakano D, Ishitsuka K, Ikeza M, Tsuchihashi R, Okawa M, Okabe H, Tamura K, Kinjo J. Screening of promising chemotherapeutic candidates from plantsagainst human adult T-cell leukemia/lymphoma (IV): phenanthroindolizidine alkaloids from Tylophora tanakae J Nat Med. 2015; 69(3): 397–401.
  • Fumiko A, Mio H, Tatsuo Y, Keiichi H, Nanao H, Mineko I, Setsuko I, Michio I. Further investigation of phenanthroindolizidine alkaloids from Tylophora tanakae. Chem Pharm Bull. 1998; 46(5): 767–769.
  • Fumiko A, Yukiko I, Tatsuo Y, Keiichi H, Nanao H. Phenanthroindolizidine alkaloids from Tylophora tanakae. Phytochemistry. 1995; 39(3): 695–699.
  • Dan S, Anne KL, Jette C, Bogdan AB, Fumiko A, Jerzy WJ. In vitro cytotoxicactivity of phenanthroindolizidine alkaloids from Cynanchum vincetoxicum and Tylophora tanakae against drug-sensitive and multidrug-resistant cancer cells. J Nat Prod. 2002; 65(9): 1299–1302.
  • Fumiko A, Mio H, Tatsuo Y, Keiichi H, Nanao H, Ritsuo N. Glycosides of 14,15-seco-and 13,14:14,15-disecopregnanes from the roots of Tylophora tanakae. Chem Pharm Bull. 1999; 47(10): 1384–1387.
  • Junei K, Daisuke N, Toshihiro F, Hikaru O. Screening of promising chemotherapeutic candidates from plants extracts. J Nat Med. 2016; 70(3): 335–360.
  • Etherington T, Herbert RB, Jackson FB. Furoquinoline alkaloids from Tylophora asthmatica. Phytochemistry. 1977; 16(7): 1125–1126.
  • Cheng-Yu C, Guo-Yuan Z, Tang-Gui X, Ping-Chuan J, Jing-Rong W, Zhi-Hong J. A phenanthroindolizidine glycoside with HIF-1 inhibitory activity from Tylophora atrofolliculata. Phytochem Lett. 2019; 31: 39–42.
  • Xue-Shi H, Shi-Shan Y, Xiao-Tian L, Nan L. A novel steroid from Tylophora atrofolliculata. J Asian Nat Prod Res. 2002; 4(3): 197–200.
  • Huang AC, Chen CY, Wang JR, Jiang ZH. Three new C21 steroidal glycosides from Tylophora atrofolliculata. Phytochem Lett. 2020; 36: 111–114.
  • Gnabre JN, Pinnas JL, Martin DG, Mizsak DA, Kloosterman DA, Baczynskyj L, Nielsen JW, Bates RB, Hoffmann JJ, Kane VV. Characterization of steroidal glycosides from Tylophora sylvatica. Tetrahedron. 1991; 47(22): 3545–3554.
  • Gnabre JN, Pinnas JL, Bates RB, Dolata DP, Gangwar S. Tylophora compounds as Na+/K+-ATPase Inhibitors. Gen Pharmacol. 1993; 24(5): 1107–1113.
  • Niaz A. Brine shrimp cytotoxicity of crude methanol extract and antispasmodic activity of α-amyrin acetate from Tylophora hirsuta BMC Complement Med. 2013; 13(135): 1–7.
  • Udupa AL, Udupa SL, Guruswamy MN. The possible site of anti-asthmatic action of Tylophora asthmatica on pituitary-adrenal axis in albino rata. Planta Med. 1991; 57(5): 409–413.
  • Haranath PS, Shyamalakumari S. Experimental study on mode of action of Tylophora asthmatica in bronchial asthma. Indian J Med Res. 1975; 63(5): 661–670.
  • Stephen J, Vijayammal PL. Antitumor activity of Tylophora asthmatica. Anc Sci Life. 2000; 20(1-2): 88–91.
  • Rabiya A, Paithankar VV, Vyas JK, Wankhade A, Kochar AC. Evaluation of antineoplastic activity of ethanolic extract of Tylophora indica. World J Pharm Med. 2020; 9(7): 1823–1830.
  • Wang YX, Jia SH, Zeng WY, Liu ZX, Fang ZJ. Effects of Tylophora ovata (Lindl) Hook. ExSteud alkaloids (TOHa) on proliferation and apoptosis of HeLa cells in vitro. Food Sci. 2008; 29(11): 609–611.
  • Malathi R, Patrick GM. Hepatoprotective activity of methanolic leaves extract of Tylophora asthmatica against paracetamol induced liver damage in rats. J Pharmacol Toxicol. 2007; 2(8): 737–742.
  • Malathi R, John SA, Cholarajan A. Tylophora asthmatica prevents lipid peroxidation in acetaminophen induced hepato toxicity in rats. Asian J Res Pharm Sci. 2011; 1(3): 71–73.
  • Ajay RC, Vijay MM, Sudhir LP, Avinash VT, Vinod SD. Effect of Tylophora asthmatica on hepatotoxicity induced by antitubercular drugs in albino rats. Int J Basic Clin Pharmacol. 2016; 5(4): 1402–1407.
  • Goun E, Cunningham G, Chu D, Nguyen C, Miles D. Antibacterial and antifungal activity of Indonesian ethnomedical plants. 2003; 74(6): 592–596.
  • Bashir A, Ali N, Bashir S, Choudhary M. Biological activities of aerial parts of Tylophora hirsute Afr J Biotechnol. 2009; 8(18): 4627–4631.
  • Maheshwari M, Vijayarengan P. Phytochemical analysis and antibacterial activities of Tylophora indica. Int J Botany Stud. 2020; 5(2): 110–114.
  • Anand M, Sharma S, Preetika M. Antimicrobial and antioxidant potential of Tylophora indica (Burm. f.) Merrill and development of an efficient micropropagation system for its therapeautic use. Med Plants Int J Phytomed. 2020; 12(1): 82–89.
  • Khanna C, Singh S, Vyas M, Das S. Evaluation of antimicrobial behaviour of Tylophora indica leaf extracts against selected food microbial pathogens. Res J Pharm Technol. 2020; 13(11): 5224–5228.
  • Sainudeen S, Nair VS, Zarbah M, Abdulla AM, Najeeb CM, Ganapathy S. Can herbal extracts serve as antibacterial root canal irrigating solutions? Antimicrobial efficacy of Tylophora indica, Curcumin longa, Phyllanthus amarus, and sodium hypochlorite on Enterococcus faecalis biofilms formed on tooth substrate: in vitro study. J Pharm Bioallied Sci. 2020; 12(1): S423–S429.
  • Raji Y, Hammed AI, Adesanwo JK, Ogunwande IA. Antiulcerogenic effects of Tylophora conspicua in male rats. Phytother Res. 2000; 14(5): 378–380.
  • Bashir A, Niaz A, Shumaila B, Jamshaid A, Sadiq A, Ibrar K. Cholinomimetic and calcium channel blocking activities of the aerial parts of Tylophora hirsuta (Wall.). J Chem Soc Pak. 2009; 31(4): 647–651.
  • Shahla N. Studies on hypoglycemic activity of Tylophora dalzellii. Pharmacologyonline. 2010; 3: 280–284.
  • Muhammad FA, Arsalan S, Ammara S, Mirza MFAB, Ali S, Azhar R, Mohammed MAD. Tylophora hirsuta leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase. Asian Pac J Trop Biomed. 2021; 11(9): 394–404.
  • Faisal R, Ali S, Bushra A, Humaira MK, Muhammed FA, Maryam Z, Abdullah M, Kashif S, Irfan H, Naeem Q. Tylophora hirsuta (Wall.) extracts ameliorate diabetes associated with inflammation in alloxan-induced diabetic rats. Endocr Metab Immune Disord Drug Targets. 2021; 21(6): 1031–1042.
  • Swathi P, Kotaiah S, Jagadeesh Kumar T. Protective effect of Tylophora indica against streptozotocin induced pancreatic and liver dysfunction in wistar rats. Biomed Pharmacol J. 2020; 13(4): 1755–1763.
  • Malathi R, Ahamed JS, Cholarajan A. Antioxidant activity of extract from the leaves of Tylophora asthmatica. J Microbiol Antimicrob. 2012; 4(4): 70–73.
  • Starlin T, Velliyur KG. Enzymatic and non-enzymatic antioxidant properties of Tylophora pauciflora Wight and Arn. an in vitro Asian J Pharm Clin Res. 2013; 6(4): 68–71.
  • Shaveta B, Atinderpal K, Manpreet K, Jatinder PS, Jobanpreet K, Srinivas N. Evaluation of anti-inflammatory activity of Tylophora asthmatica. Curr Trends Biotechnol Pharm. 2021; 15(6): 90–95.
  • Gupta V, Guleri R, Gupta M, Kaur N, Kaur K, Kumar P, Anand M, Gurcharan K, Pratap Kumar P. Anti-neuroinflammatory potential of Tylophora indica (Burm. f) Merrill and development of an efficient in vitro propagation system for its clinical use. PLoS One. 2020; 15(3): 1–20.
  • Cheng-Wei Y, Yue-Zhi L, Iou-Jiun K, Dale LB, Jia-Tsrong J, Du L, Chun-Wei H, Teng-Kuang Y, Yu-Sheng C, Shiow-Ju L. Identification of phenanthroindolizines and phenanthroquinolizidines as novel potent anti-corona viral agents for porcine enteropathogenic coronavirus transmissible gastroenteritis virus and human severe acute respiratory syndrome corona virus. Antiviral Res. 2010; 88(2): 160–168.
  • Dhadde Gurunath S, Mali Hanmant S, Sapate Rohit B, Vakhariya Rohan R, Raut Indrayani D, Nitalikar Manoj M. Investigation of in vitro anthelmintic activity of methanolic extract of Tylophora indica leaves against Haemonchus contortus. J Univ Shanghai Sci Technol. 2021; 23(2): 37–42.
  • Kumara Swamy M. Plant-derived bioactives: production, properties and therapeutic applications. 1st Singapore: Springer, 2020.
  • Junmin Z, Susan LM, Di M, Xiao-Fei S, Chen-Jie Y, Ying-Qian L, Kuo-Hsiung L. Biologically active indolizidine alkaloids. Med Res Rev. 2021; 41(2): 928–960.