Investigation of Crucial Affected Proteins in Rat Liver in the Presence of Scrophularia

Document Type : Original paper


1 Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2 Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

3 Traditional Medicine and Materia Medica Research Center, Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

4 Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

5 Department of Surgery, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.



Background and objectives: Radix Scropholaria is dried root Scrophularia ningpoensis Hemsl. which is used uses as a drug against several diseases. In the present study, the crucial affected proteins of rat liver in the presence of radix Scrophularia have been investigated. Methods: The differentially expressed proteins (DEPs) were downloaded from literature. The significant DEPs plus 100 added first neighbors were determined and included in “protein query” of STRING database via Cytoscape software. The network was analyzed and the central nodes among the queried DEPs were identified. The 10 first neighbors of the central DEPs were determined. Results: RT1-CE12, Gfer, Serpina3c, Rab13, Rbm14, Ighg Psmb8, COX2, Olr796, Mga, Ugt1a6, Ugt2b, Ebpl, Ugt2b, Igf2r, and Amacr as significant DEPs were analyzed via protein-protein interaction (PPI) network analysis, Ugt1a1 and Ugt2b the two up-regulated proteins were highlighted as the crucial proteins in response to the radix Scrophularia. Conclusion: Two members of UDP glucuronosyltransferase family; Ugt1a1 and Ugt2b, were pointed as the critical liver enzyme which are dysregulated under effect of radix Scrophulariae. Due to crucial role of Ugt1a1 in the liver function, it is suggested that consumption of Scrophularia ningpoensis Hemsl. as a traditional medicine required more investigation.


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