Allium affine Extract Improves Dexamethasone-Induced Hyperlipidemia in Rats

Document Type : Original paper

Authors

1 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

2 Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

10.22127/rjp.2023.375545.2019

Abstract

Background and objectives: Various Alliumplants were found to improve blood lipid profile. The present investigation explored the anti-hyperlipidemic potential of A. affine in a rat model of hyperlipidemia induced by glucocorticoid. Methods: Hydroalcoholic extract was prepared by maceration method and assessed for total phenolic content. Forty-eight male Wistar rats in eightgroups were studied. Group I received vehicle; group II was treated only with 400 mg/kg A. affine orally; group III was subcutaneously injected with 10 mg/kg/day dexamethasone; group IV as the reference group received dexamethasone and 40 mg/kg atorvastatin orally; groups V-VIII were treated with dexamethasone and simultaneously with 50, 100, 200 or 400 mg/kg of A. affine extract orally. All treatments were done over a period of seven days. Blood levels of glucose, lipid profile, liver enzymes and malondialdehyde (MDA) were assessed in over-fasted rats. Liver tissues were weighed and evaluated for histopathologicalalterations. Results: The amount of total phenolics content of A. affine extract was 11.24 ± 1.7 mg/g as gallic acid equivalent. Administration of A. affineextract significantly lowered the blood levels of triglycerides, total cholesterol, low-density lipoprotein (LDL)-cholesterol, very low-density lipoprotein (VLDL)-cholesterol, blood sugar, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and MDA. However, there was no significant effect on high-density lipoprotein (HDL)-cholesterol level. A. affineimproved hepatic steatosis resulted from dexamethasone. Conclusion: These findings suggest potential of A. affine for preventing and managing hyperlipidemia. However, more trials are needed concerning its clinical efficacy and identifying its bioactive phytochemicals and mechanisms participated in the lipid-lowering action.

Keywords

References

  • Shattat GF. A review article on hyperlipidemia: types, treatments and new drug targets. Biomed Pharmacol J. 2015; 7(1): 399–409.
  • Hasani Ranjbar S, Nayebi N, Moradi L, Mehri A, Larijani B, Abdollahi M. The efficacy and safety of herbal medicines used in the treatment of hyperlipidemia; a systematic review. Curr Pharm Des. 2010; 16(26): 2935–2947.
  • Rafieian Kopaei M, Setorki M, Doudi M, Baradaran A, Nasri H. Atherosclerosis: process, indicators, risk factors and new hopes. Int J Prev Med. 2014; 5(8): 927–946.
  • Rizzo M, Kotur Stevuljevic J, Berneis K, Spinas G, Rini GB, Jelic Ivanovic Z, Spasojevic Kalimanovska V, Vekic J. Atherogenic dyslipidemia and oxidative stress: a new look. Transl Res. 2009; 153(5): 217–223.
  • Ismail T, Soliman M, Nassan M, Mohamed Antihypercholesterolemic effects of mushroom, chrysin, curcumin and omega-3 in experimental hypercholesterolemic rats. J Food Nutr Res. 2015; 3(2): 77–87.
  • Rauf A, Akram M, Anwar H, Daniyal M, Munir N, Bawazeer S, Bawazeer S, Rebezov M, Bouyahya A, Shariati MA, Thiruvengadam M. Therapeutic potential of herbal medicine for the management of hyperlipidemia: latest updates. Environ Sci Pollut Res Int. 2022; 29(27): 40281–40301.
  • Ha W, Ying T, Kim W. The effects of black garlic (Allium satvium) extracts on lipid metabolism in rats fed a high fat diet. Nutr Res Pract. 2015; 9(1): 30–36.
  • Rahman K, Lowe GM. Garlic and cardiovascular disease: a critical review. J Nutr. 2006; 136(S3): 736–740.
  • Namin HH, Mehrvarz SS, Zarre S. Scape anatomy of Allium (Alliaceae) in Iran and its systematic application. Thaiszia J Bot. 2009; 19(1): 37–45.
  • Sadeghi M, Safaeian L, Ghazvini MA, Ramezani M. Evaluation of fibrinolytic and antioxidant effects of Allium affine hydroalcoholic extract. Res Pharm Sci. 2017; 12(4): 299–306.
  • Kazemi M, Zolfaghari B, Keivanloo Shahrestanaki SM, Sadeghi Dinani M. Cytotoxic effects of Allium affine Ledeb butanolic fraction on breast and ovary cancer cell lines. J Med Plants. 2018; 4(64): 83–90.
  • Ross IL, Marais AD. The influence of glucocorticoids on lipid and lipoprotein metabolism and atherosclerosis. S Afr Med J. 2014; 104(10): 671–674.
  • Noubiap JJ, Nansseu JR, Lontchi Yimagou E, Nkeck JR, Nyaga UF, Ngouo AT, Tounouga DN, Tianyi FL, Foka AJ, Ndoadoumgue AL, Bigna Global, regional, and country estimates of metabolic syndrome burden in children and adolescents in 2020: a systematic review and modelling analysis. Lancet Child Adolesce Health. 2022; 6(3): 158–170.
  • Abdelhady MI, Motaal AA, Beerhues L. Total phenolic content and antioxidant activity of standardized extracts from leaves and cell cultures of three Callistemon Am J Plant Sci. 2011; 2(6): 847–850.
  • Feghhi Najafabadi S, Safaeian L, Zolfaghari B. In vitro antioxidant effects of different extracts obtained from the leaves and seeds of Allium ampeloprasum persicum. J Herb Med Pharmacol. 2019; 8(3): 256–260.
  • Kumar VS, Inamdar MN, Viswanatha GL. Protective effect of lemongrass oil against dexamethasone induced hyperlipidemia in rats: possible role of decreased lecithin cholesterol acetyl transferase activity. Asian Pac J Trop Med. 2011; 4(8): 658–660.
  • Momi S, Impagnatiello F, Guzzetta M, Caracchini R, Guglielmini G, Olivieri R, Monopoli A, Gresele NCX 6560, a nitric oxide-releasing derivative of atorvastatin, inhibits cholesterol biosynthesis and shows anti-inflammatory and anti-thrombotic properties. Eur J Pharmacol. 2007; 570(1-3): 115–124.
  • Pragda S, Kuppast I, Mankani K, Ramesh L. Evaluation of antihyperlipidemic activity of leaves of Portulaca oleracea Linn against dexamethasone induced hyperlipidemia in rats. Int J Pharm Pharm 2012; 4(4): 279–283.
  • Safaeian L, Zolfaghari B, Karimi S, Talebi A, Ghazvini MA. The effects of hydroalcoholic extract of Allium elburzense Wendelbo bulb on dexamethasone-induced dyslipidemia, hyperglycemia, and oxidative stress in rats. Res Pharm Sci. 2018; 13(1): 22–29.
  • Hassan NF, Soliman GM, Okasha EF, Shalaby AM. Histological, immunohistochemical, and biochemical study of experimentally induced fatty liver in adult male albino rat and the possible protective role of pomegranate. J Microsc Ultrastruct. 2018; 6(1): 44–55.
  • Chang TC, Chang HT, Chang ST, Lin SF, Chang YH, Jang HD. A comparative study on the total antioxidant and antimicrobial potentials of ethanolic extracts from various organ tissues of Allium Food Sci Nutr. 2013; 4(8): 182–190.
  • Beato VM, Orgaz F, Mansilla F, Montaño A. Changes in phenolic compounds in garlic (Allium sativum) owing to the cultivar and location of growth. Plant Foods Hum Nutr. 2011; 66(3): 218–223.
  • Benkeblia N. Free-radical scavenging capacity and antioxidant properties of some selected onions (Allium cepa) and garlic (Allium sativum L.) extracts. Braz Arch Biol Technol. 2005; 48(5): 753–759.
  • Wang JC, Gray NE, Kuo T, Harris CA. Regulation of triglyceride metabolism by glucocorticoid receptor. Cell Biosci. 2012; 2(1): 1–9.
  • Du WW, Liu F, Shan SW, Ma XC, Gupta S, Jin T, Spaner D, Krylov SN, Zhang Y, Ling W, Yang Inhibition of dexamethasone-induced fatty liver development by reducing miR-17-5p levels. Mol Ther. 2015; 23(7): 1222–1233.
  • Wang M. The role of glucocorticoid action in the pathophysiology of the metabolic syndrome. Nutr Meta. 2005; 2(1): 1–4.
  • Dourakis SP, Sevastianos VA, Kaliopi P. Acute severe steatohepatitis related to prednisolone therapy. Am J Gastroenterol. 2002; 97(4): 1074–1075.
  • Arnaldi G, Scandali VM, Trementino L, Cardinaletti M, Appolloni G, Boscaro M. Pathophysiology of dyslipidemia in Cushing’s syndrome. Neuroendocrinology. 2010; 92(S1): 86–90.
  • Geer EB, Islam J, Buettner C. Mechanisms of glucocorticoid-induced insulin resistance: focus on adipose tissue function and lipid metabolism. Endocrinol Metab Clin North Am. 2014; 43(1): 75–102.
  • Al-Numair KS. Hypocholesteremic and antioxidant effects of garlic (Allium sativum) extract in rats fed high cholesterol diet. Pak J Nutr. 2009; 8(2): 161–166.
  • Vidyavati G, Manjunatha H, Hemavathy J, Srinivasan K. Hypolipidemic and antioxidant efficacy of dehydrated onion in experimental rats. J Food Sci Technol. 2010; 47(1): 55–60.
  • Sobenin IA, Pryanishnikov VV, Kunnova LM, Rabinovich YA, Martirosyan DM, Orekhov AN. The effects of time-released garlic powder tablets on multifunctional cardiovascular risk in patients with coronary artery disease. Lipids Health Dis. 2010; 9(1): 1–6.
  • Ried K, Toben C, Fakler P. Effect of garlic on serum lipids: an updated meta-analysis. Nutr Rev. 2013; 71(5): 282–299.
  • Pin Y, Lin L, Yeh H. Antihyperlipidemic activity of Allium chinense J Food Drug Anal. 2016; 24(3): 516–526.
  • Corzo Martínez M, Corzo N, Villamiel M. Biological properties of onions and garlic. Trends Food Sci Technol. 2007; 18(12): 609–625.
  • Li W, Yang C, Mei X, Huang R, Zhang S, Tang Y, Dong Q, Zhou C. Effect of the polyphenol‐rich extract from Allium cepa on hyperlipidemic sprague‐dawley rats. J Food Biochem. 2021; Article ID 13565.
  • Sobolewska D, Michalska K, Podolak I, Grabowska K. Steroidal saponins from the genus Allium. Phytochem Rev. 2016; 15(1): 1–35.
  • Matsuura H. Saponins in garlic as modifiers of the risk of cardiovascular disease. J Nutr. 2001; 131(3S): 1000–1005.
  • Michel C, Cabanac M. Effects of dexamethasone on the body weight set point of rats. Physiol Behavior. 1999; 68(1-2): 145–150.
  • Masjedi F, Gol A, Dabiri S. Preventive effect of garlic (Allium sativum) on serum biochemical factors and histopathology of pancreas and liver in streptozotocin-induced diabetic rats. Iran J Pharm Res. 2013; 12(3): 325–338.
  • Guan MJ, Zhao N, Xie KQ, Zeng T. Hepatoprotective effects of garlic against ethanol-induced liver injury: a mini-review. Food Chem Toxicol. 2018; 111: 467–473.
  • Dunford EC, Riddell MC. The metabolic implications of glucocorticoids in a high-fat diet setting and the counter-effects of exercise. Metabolites. 2016; 6(4): 1–14.
Research Journal of Pharmacognosy
Volume 10, Issue 2
April 2023
Pages 9-17

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