In vivo wound healing activity of a herbal ointment in rat

Document Type : Original paper


1 Neurophysiology Research Center, Shahed University, Tehran, Iran. Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran

2 Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.

3 Department of Pathology, School of Medicine, Shahed University, Tehran, Iran.

4 Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran.


Background and objectives: The wounds are still the health tribulations at the present time. In the present research the effect of a new herbal ointment (Oppyheal) in treatment of rat’s wound has been investigated. The effectiveness of the ointment was compared with the Fibrinolysin/DNAse.
Methods: Fifty four male Wistar rats were used. The wound was created on the back cervical skin of the animals under anesthesia in three different sizes. The animals in each size group were randomly divided into three groups. The control group did not receive the ointments. The reference group was given the Fibrinolysin/DNAse and the third group was treated with the Oppyheal. The products were topically used once per day until the wounds of one group were completely healed. The size of the wound area was measured in days 0-20 by a standard reference ruler. The reduction in size of the wound was calculated and analyzed. The recovered skin of all animals were examined histologically, pResults: The results showed a significant difference in wound contraction between the treated groups and the control group (p<0.0001). The new skin of ointment-treated rats showed healing features in comparison to the control group.
Conclusion: This study may introduce a suitable topical ointment, Oppyheal, for wound care.


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