Holothurin B Isolated from Holothuria atra Inhibits Angiogenesis More Potent than Curcumin in Vitro

Document Type : Original paper


1 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

2 Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

3 Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

4 Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.


Background and objectives: Triterpene glycosides as the most bioactive components of sea cucumbers, have been considered for their various pharmacological properties especially anticancer and anti-metastasis activities. Due to the limited information on the biological properties of holothurin B as a marine triterpene glycoside, the present study aimed to examine its effect on angiogenesis and compare it with curcumin usinghuman umbilical vein endothelial cells (HUVECs). Methods: Holothurin B was isolated from Holothuria atra and identified by NMR and Mass spectroscopic data. Cell survival was estimated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique and migration of cells was assessed by Transwell test. Angiogenesis was evaluatedin vitro by tube formation assay. Results: Holothurin B reduced HUVECssurvival with IC50 value of 8.16 µg/mL. At the concentrations of 5 and 7.5 µg/mL, it significantly decreased the number of migrated cells, the average length and size of tubules, and mean number of junctions; it was more potent than curcumin. Conclusion: Holothurin B could be considered as a potent antiangiogenic constituent through suppressing endothelial cell proliferation, migration and tubulogenesis in vitro, suggesting its potential for further animal and clinical investigations.


Main Subjects

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