Optimization of Ultrasound-assisted Acidic-solvent Extraction of Colchicine from Colchicum kurdicum (Bornm.) Stef. Using Response Surface Methodology

Document Type : Original paper


1 Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

2 Pharmaceutical Cares Department, Ayatollah Khamenei Hospital, Mazandaran University of Medical Sciences, Abbas Abad, Iran.

3 Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

4 Department of Plant Systematics, High Educational of Sanna Institute, Sari, Iran.

5 Department of Medicinal Chemistry and Pharmaceutical Sciences Research Centre, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

6 Active Pharmaceutical Research Centre (APIRC), Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.


Background and objectives: Colchicum kurdicum (Bornm.) Stef. is a flowering perennial monocotyledon plant that has many important bioactive compounds especially colchicine and colchicine derivatives. In this study, the ultrasound-assisted acidic-solvent extraction method coupled with response-surface method was presented as the successful method for large scale extraction of colchicine as an alkaloid compound from Colchicum kurdicum. Methods: According to the literatures, methanol/deionized water (70:30) solvent system was selected for the extraction. In addition, the response-surface method was used for analysis and optimization of colchicine extraction by ultrasonic-assisted acidic-solvent extraction method. Subsequently, colchicine was extracted using this method and the effects of solvent pH, extraction time, solvent/plant ratio, power, and temperature were evaluated. Results: After all analysis procedures, 0.99 mg colchicine/g dried corms was achieved with the following conditions: solvent pH 4, Extraction time 120 minutes, solvent/plant ratio 20 mL/g, power 100 W, and temperature 60 ºC. Conclusion: According to this study, ultrasonic-assisted acidic-solvent extraction was found an effective method for extraction of colchicine from Colchicum kurdicum (Bornm.) Stef. compared to other extraction methods.


Main Subjects

[1] Kahraman A, Celep F. Anatomical properties of Colchicum kurdicum (Bornm.) Stef. (Colchicaceae). Aust J Cr Sci. 2010; 4(5): 369-371.
[2] Fakhri M, Davoodi A, Parviz M, Sadeghi Ghadi Z, Mousavinasab SN, Farhadi R, Azadbakht M, Azadbakht M. Characterization and HPLC analysis of manna from some Cotoneaster species. Int J Pharm Sci Res. 2017; 8(12): 1000-1006.
[3] Aghili Alavi Khorasani MH. Makhzan al-advieh. Tehran: Tehran University of Medical Sciences, 2009.
[4] Choopani R, Ghourchian A, Hajimehdipoor H, Kamalinejad M. Scientific evaluation of pharmacological treatment of osteoarthritis in the Canon of medicine. J Evid Based Complement Altern Med. 2016; 21(3): 228-234.
[5] Khan H, Tariq SA, Khan MA. Biological and phytochemical studies on corms of Colchicum luteum Baker. J Med Plant Res. 2011; 5(32): 7031-7035.
[6] Gulsoy-Toplan G, Goger F, Yildiz-Pekoz A, Gibbons S, Sariyar G, Mat A. Chemical constituents of the different parts of Colchicum micranthum and C. chalcedonicum and their cytotoxic activities. Nat Prod Commun. 2018; 13(5): 535-538.
[7] Davoodi A, Khoshvishkaie E, Azadbakht M. Plant cells technology as an effective biotechnological approach for high scale production of pharmaceutical natural compounds; a meta-analysis study. Pharm Biomed Res. 2019; 5(2): 1-9.
[8] Tahmasebi-Boldaji R, Hatamipour MS, Khanahmadi M, Sadeh P, Najafipour I. Ultrasound-assisted packed-bed extraction of hypericin from Hypericum perforatum L. and optimization by response surface methodology. Ultrason Sonochem. 2019; 57(1): 89-97.
[9] Amiri S, Shakeri A, Sohrabi MR, Khalajzadeh S, Ghasemi E. Optimization of ultrasonic assisted extraction of fatty acids from Aesculus hippocastanum fruit by response surface methodology. Food Chem. 2019; 271(1): 762-766.
[10] Rambo DF, Biegelmeyer R, Toson NSB, Dresch RR, Moreno PRH, Henriques AT. Box-Behnken experimental design for extraction optimization of alkaloids from Erythrina verna Vell. trunk barks and LC method validation. Ind Crops Prod. 2019; 133(1): 250-258.
[11] Baş D, Boyacı IH. Modeling and optimization I: usability of response surface methodology. J Food Eng. 2007; 78(3): 836-845.