Anti-Anxiety Effect of Salvia hypoleuca

Document Type : Short communication


1 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

2 Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.

3 Department of Pharmacognosy, Faculty of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran

4 Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

5 Department of Pharmacognosy and Biotechnology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.


 Background and objectives: Anxiety is a chronic and common disorder worldwide and impairs the quality of life of affected people. Herbal medicines have long been used to treat CNS related disorders. Salvia species are important medicinal plants that have shown various pharmacologic activities including CNS effects. Salvia hypoleuca is an annual and herbaceous plant which is endemic to Iran. The aim of this study was to evaluate the anxiolytic activity of the hydro-alcoholic, n-hexane, ethyl acetate and methanol extract of S. hypoleuca in mice. Methods: n-hexane, ethyl acetate, methanol and hydroalcholic extracts were prepared from aerial parts of Salvia hypoleuca. Anxiolytic activity of the extracts was evaluated using open field and hole-board tests. The mice were randomly divided into different groups and were treated with normal saline, diazepam, and the extracts of Shypoleuca (500 mg/kg, ip). Results: The results of open field and hole-board tests showed that treatment of mice with the hydro-alcoholic and methanol extracts of Shypoleuca(500 mg/kg, ip) produced a significant anxiolytic effect as compared to the normal control group.  Conclusion: This study demonstrated that the hydro-alcoholic and methanol extracts of aerial parts of S. hypoleuca have anti-anxiety activity. The isolation and identification of the active compounds of the effective extracts are suggested.


Main Subjects

[1] Mahendra P, Bisht S. Anti-anxiety activity of Coriandrum sativum assessed using different experimental anxiety models. Indian J Pharmacol. 2011; 43(5): 574-577.
[2] Chandrashekar K, Amudha P, Venkataraman S. Anxiolytic activity of ethanolic and aqueous extract of Ficus carica Linn. fruits in swiss albino mice. Int J Basic Clin Pharmacol. 2017; 6(8): 2043-2050.
[3] Shah G, Shiri R, Dhabiliya F, Nagpal N, Mann AS. Anti-anxiety activity of Cymbopogon citratus (dc.) Stapf leaves extracts on the elevated plus-maze model of anxiety in mice. Phcog J. 2010; 2(15): 45-50.
[4] Bonyani A, Sajjadi SE, Rabbani M. Anxiolytic effects of Lippia citriodora in a mouce model of anxiety. Res Pharm Sci. 2018; 13(3): 205-212.
[5] Imanshahidi M, Hosseinzadeh H. The pharmacological effects of Salvia specieson the central nervous system. Phytother Res. 2006; 20(6): 427-437.
[6] Nickavar B, Mojab F, Asgarpanah J. Volatile composition of the essential oil of Salvia hypoleuca Benth. Int J Aromather. 2005; 15(1): 51-53.
[7] Estakhr J, Javdan N, Motalleb G, Sanchooli N, Marzban N, Shams Lahijani M, Nikravesh A, Rostamian V. Evaluation of the effects of Salvia hypoleuca on the cAMP-responsive element modulator (CREM) gene expression and spermatogenesis in rat. Middle East Fertile Soc J. 2010; 15(4): 274-277.
[8] Firuzi O, Miri R, Asadollahi M, Eslami S, Jassbi AR. Cytotoxic, antioxidant and antimicrobial activities and phenolic contents of eleven Salvia species from Iran. Iran J Pharm Res. 2013; 12(4): 801-810.
[9] Karami M, Shamerani MM, Hossini E, Gohari AR, Ebrahimzadeh MA, Nosrati A. Antinociceptive activity and effect of methanol extracts of three Salvia spp. on withdrawal syndrome in mice. Adv Pharm Bull. 2013; 3(2): 457-459.
[10] Moridi Farimani M, Miran M, Nejad Ebrahimi S. New diterpenoids from the aerial parts of Salvia reuterana. Iran J Pharm Res. 2019; 18(1): 406-411.
[11] Sturman O, Germain PL, Bohacek J. Exploratory rearing: a context- and stress-sensitive behavior recorded in the open-field test. Stress. 2018; 21(5): 443-452.
[12] Takeda H, Tsuji M, Matsumiya T. Changes in head-dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. Eur J Pharmacol. 1998; 350(1): 21-29.
[13] Sarris J, McIntyre E, Camfield DA. Plant-based medicines for anxiety disorders, part 2: a review of clinical studies with supporting preclinical evidence. CNS Drugs. 2013; 27(4): 301-319.
[14] Naderi N, Akhavan N, Aziz Ahari F, Zamani N, Kamalinejad M, Shokrzadeh M, Ahangar N, Motamedi F. Effects of hydroalcoholic extract from Salvia verticillata on pharmacological models of seizure, anxiety and depression in mice. Iran J Pharm Res. 2011; 10(3): 535-545.
[15] Rabbani M, Sajjadi SE, Jafarian A, Vaseghi G. Anxiolytic effects of Salvia reuterana Boiss. on the elevated plus-maze model of anxiety in mice. J Ethnopharmacol. 2005; 101(1-3): 100-103.
[16] Maione F, Bonito MC, Colucci M, Cozzolino V, Bisio A, Romussi G, Cicala C, Pieretti S, Mascolo N. First evidence for an anxiolytic effect of a diterpenoid from Salvia cinnabarina. Nat Prod Commun. 2009; 4(4): 469-472.
[17] Braida D, Capurro V, Zani A, Rubino T, Vigano D, Parolaro D, Sala M.  Potential anxiolytic- and antidepressant-like effects of salvinorin A, the main active ingredient of Salvia divinorum, in rodents. Br J Pharmacol. 2009; 157(5): 844-853.