- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001The Effects of Inhaled Limnophila aromatica Essential Oil on Brain Wave Activities and Emotional States in Healthy Volunteers: a Randomized Crossover Study1911363410.22127/rjp.2020.230400.1586ENPakamon ThanatuskittiCollege of Public Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Faculty of Medicine, Mahasarakham University, Maha Sarakham, Thailand.Vorasith SiripornpanichResearch Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Nakhonpathom, Thailand.Winai SayorwanKanchanabhisek Institute of Medical and Public Health Technology, Nontaburi, Thailand.Chanida Palanuvej*College of Public Health Sciences, Chulalongkorn University, Bangkok, Thailand.0000-0003-2688-8784Nijsiri RuangrungsiCollege of Public Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Department of Pharmacognosy, College of Pharmacy, Rangsit University, Pathum Thani, Thailand.Journal Article20200508<strong>Background and objectives</strong>: <em>Limnophila aromatica</em> (Lam.) Merr., a characteristic odorous vegetable, is commonly used in Thai dishes and folk medicine. <em>Limnophila aromatica</em> essential oil has never been investigated on brain activities before. This study aimed to evaluate the effects of <em>L. aromatica</em> essential oil inhalation on brain wave activities through EEG recordings and emotional states among healthy volunteers using a randomized crossover design. <strong>Methods:</strong> <em>Limnophila</em><em> aromatica</em> essential oil was extracted from the aerial parts by hydro-distillation method and its chemical components were analyzed by GC/MS. The essential oil was diluted to 8% v/v with sweet almond oil. Twenty-four healthy participants aged between 18 and 25 years, recruited from general public, inhaled both <em>L. aromatica </em>essential oil and sweet almond oil with seven-day washout period. Nicolet EEG v32 was used to record brain activities and the self-evaluated questionnaire on emotional states in Thai version of The Geneva Emotion and Odor Scale was used to measure the subjective feelings of the participants. Data were analyzed using paired t-test with a significance level of 0.05. <strong>Results: </strong><em>Limnophila</em> aromatica essential oil icreases theta and alpha wave and induced more relaxed feelings compared to the vehicle. <strong>Conclusion:</strong> The inhalation of <em>L. aromatica</em> essential oil could cause the relaxing effects on positive mood states and the relaxation of brain state.https://www.rjpharmacognosy.ir/article_113634_27b95d7dbbd6ef9695da7af9bfd41c1f.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Comparing the Effects of a Herbal Ointment (Based on Persian Medicine) and Silver Sulfadiazine Ointment on the Second-Degree Burn Wounds: a Single-Blind Randomized Clinical Trial112211142210.22127/rjp.2020.229457.1580ENMehdi AlizadehDepartment of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-6756-4447Mostafa DahmardeheiBurn Research Center, Iran University of Medical Sciences, Tehran, Iran.Shirin FahimiTraditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0003-2443-7621Sajjad SadeghiIranian Traditional Medicine Clinic, Amiralmomenin Hospital, Semnan University of Medical Sciences, Semnan, Iran.0000-0003-3241-0736Roshanak Mokaberinejad*Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
No.8 Shams Alley, Vali-e-Asr Street, Tehran, Iran.0000-0002-8851-5572Journal Article20200503<strong>Background and Objective: </strong>Burn injuries impose heavy costs on the healthcare systems. Since the available treatments for burn injuries are costly and have several complications, the present study aimed to compare the effects of an Iranian traditional medicine product in the form of a herbal ointment with silver sulfadiazine ointment on second-degree burn wounds. <strong>Methods:</strong> In this single-blind randomized clinical trial, the patients were divided into two groups of herbal ointment (<em>Rosa damascena</em>, <em>Solanum nigrum</em>, and <em>Malva sylvestris</em>) and SSD ointment (control). They were evaluated in terms of improvement, wound closure, wound appearance, and intervention complications prior to the treatment, on the fourth day, and after 1-4 weeks. <strong>Results:</strong> Fifty three participants completed the trial (27 in herbal ointment and 26 in SSD group). The mean burn wound healing time was 11.58±5.36 and 16.80±5.60 days in the herbal and SSD groups, respectively, which showed a significant difference in this regard (p=0.001). Moreover, the wound closure percentage in the first two weeks was significantly higher in the herbal group compared to the SSD group (p <0.05). Also, wound appearance was better in the herbal ointment group in all the assessment times. No significant difference was observed between the herbal and SSD ointment regarding the treatment complications. <strong>Conclusion:</strong> The herbal ointment containing <em>Rosa damascena</em>, <em>Malva sylvestris</em>, and <em>Solanum nigrum</em> showed more considerable effects on the second-degree burn wounds compared to the SSD ointment.https://www.rjpharmacognosy.ir/article_111422_70fca2c2ef9f62cd686a08243ca5434f.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Comparative Effects of Mouthwash Containing Trachypermum ammi Oil with Chlorhexidine on Dental Plaque Induced Gingivitis233011342810.22127/rjp.2020.113428ENAnna SaffarpourSchool of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran.
Department of Periodontology, International Campus, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.Department of Periodontology, International Campus, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.0000-0002-7464-7542Niloofar AminiDepartment of Periodontology, International Campus, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.0000-0003-2282-7432Abbas KeshtkarDepartment of Health Science Educational Development, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.0000-0002-7305-8639Najmeh Mokhber-DezfuliMedicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.0000-0003-1397-8289Azadeh Manayi*Medicinal Plants Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.0000-0003-4414-4818Journal Article20200526<strong>Background and objectives: </strong>The primary etiology for gingivitis is dental plaque resulting in gum inflammation. Beside mechanical procedures, chemical agents in mouthwash can help in removal of plaques. Oil of <em>Trachyspermum ammi</em> rich in thymol may provide beneficial effect in control of gingivitis; therefore, the present study aimed to compare the effect of mouthwash containing the oil with chlorhexidine to control dental plaque and inflammation of gingivae. <strong>Methods: </strong>Essential oil of the plant was analyzed using GC-MS and amount of thymol was assayed by GC method. Herbal mouthwash was prepared and applied in an open-label non-randomized study, adult female and male suffering of gingivitis (N=30) entered in two groups after oral prophylaxis. Examining for plaque index (PI), bleeding index (BI), and modified gingival index (MGI) were conducted after two weeks administration of the herbal mouthwash.<strong> Results: </strong>The herbal mouthwash was stable for two months with no physical change and microbial contamination (0.05). <strong>Conclusion:</strong> The effect of <em>T. ammi</em> oil in control of inflammation was comparable with chlorhexidine, while chlorhexidine reduced risks of PI, BI, and MGI higher than those receiving the herbal mouthwash.https://www.rjpharmacognosy.ir/article_113428_1dfe791e54a3ad6262bfe6a921c178e0.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Phytochemical and Anti-Inflammatory Analysis of Prunus africana Bark Extract313811366110.22127/rjp.2020.229941.1583ENGitonga Godfrey Mutuma*Department of Medical Laboratory Sciences, School of Public Health, Biomedical Sciences and Technology, Masinde Muliro University of Science and Technology, Kakamega, Kenya.0000-0003-1891-1817Joseph NgeranwaDepartment of Biochemistry, Microbiology and Biotechnology, Kenyatta University, Nairobi, Kenya.0000-0002-1158-5521Machocho Alex Kin G’oriDepartment of Chemistry, Kenyatta University, Nairobi, Kenya.Silas KirukiDepartment of Physical Sciences, Chuka University, Chuka, Kenya.Journal Article20200505<strong>Background and objectives:</strong> Inflammation is associated with various diseases;<em> Prunus africana</em> (Hook f.) is commonly used in Meru community of Kenya in management of inflammation. Medicinal plants contain phytochemicals associated with pharmacological activities; so, the aim of the present study was evaluating the anti-inflammatory activity of <em>Prunus africana</em> bark extract and qualitative analysis phytochemical of its phytochemicals. <strong>Methods:</strong> Five hundred mg of the powdered <em>P. africana</em> stem bark was extracted using 1.5 liters of dichloromethane for 24 h. The anti- inflammatory activity was evaluated against carrageenan paw induced edema in mice. The ability of the extracts to suppress the paw inflammation was expressed as a percentage inhibition of paw edema in five groups each comprising of five mice. Group I was treated with DMSO, group II with diclofenac (100 mg/Kg) and experimental groups III, IV and V with 50, 100 and 150 mg/Kg of the plant extract. The ability of the extracts to suppress the paw inflammation was expressed as a percentage inhibition of paw edema in mice. The qualitative phytochemical analysis was conducted using the standard protocols. <strong>Results:</strong> The percentages paw edema inhibition after the 4<sup>th</sup> h in the positive control and the experimental groups I, II and III were 13.61, 32.85, 25.15 and 5.92%, respectively. The qualitative evaluation of stem bark extract illustrated presence of tannins, saponins, flavonoids, alkaloids, quinones, cardiac glycosides, terpenoids, phenolics and coumarins. <strong>Conclusion:</strong> Dichloromethane stem bark extract of the <em>P. africana</em> presented anti-inflammatory activity hence a possible candidate for extraction of active anti-inflammatory compounds. <br /> <br /> <br /> https://www.rjpharmacognosy.ir/article_113661_7a6ef285246815770b2ad69b7b85281c.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Formulation and Finger Printing of a Poly Herbal Film-Coated Tablet for Treatment of Hemorrhoids394711415610.22127/rjp.2020.238203.1609ENSahar DehdariDepartment of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-7178-4493Homa Hajimehdipoor*Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-2739-3399Somayeh EsmaeiliTraditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0003-1140-0790Seyed Alireza MortazaviDepartment of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Rasool ChoopaniDepartment of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-5550-4543Journal Article20200705<strong>Background and objectives: </strong>Hemorrhoids is the most prevalent rectal disease. Despite different medical efforts, its complications are not managed well. In the present research, a popular prescription for treatment of hemorrhoids was formulated as tablet dosage form and, its HPTLC fingerprint prepared<em>. </em><strong>Methods: </strong><em>Commiphora mukul</em> was dissolved in <em>Allium ampeloprasum</em> juice (1:3). Then, this solution was blended with <em>Terminalia chebula</em>, <em>Phyllantus emblica</em> and <em>Terminalia bellirica</em> (1:1:1) powder. Different formulations were prepared from the mixture and the best one was selected for tablet preparation. Subsequently, the tablets were coated and their physicochemical characteristics and fingerprint pattern were obtained using silica gel plate, NP/PEG reagent and toluene: ethyl acetate: formic acid (70:15:15) as mobile phase. Laboratory stability studies were carried out as well. <strong>Results:</strong> Formulation C revealed excellent results in flowability studies (angle of repose: 26, carr’s index: 6, hausner ratio: 1.00). It was also demonstrated acceptable results in different tests including weight variation (500 mg), hardness (8.04 kg/cm<sup>2</sup>), disintegration time (28.50 min), friability (0.6%), dissolution (97.6% phenolics and 96.1% tannins, respectively) and the coating process. Total phenolics and tannins contents were determined as 125.8 mg/tab and 89.2 mg/tab, respectively. In fingerprinting study, characteristic spots of each species were distinguished. The film-coated tablets were stable in laboratory stability test. <strong>Conclusion:</strong> With reference to anti-inflammatory, astringent and wound healing roles of phenolics and tannins in hemorrhoids, the present tablets could be an appropriate candidate for hemorrhoids regarding its historical backgrounds.https://www.rjpharmacognosy.ir/article_114156_0d414005ff7dca2cf59d6edcb2d524ce.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Improvement of Blood Glucose, Serum Antioxidant Status and Liver Enzyme Changes by Hydroalcoholic Extract of Solanum melongena Green Cap in Diabetic Rats495511398110.22127/rjp.2020.232620.1594ENFatemeh KhazaeiStudent Research Committee, Kermanshah University of Medical Sciences, Kermanshah, Iran0000-0002-1284-3676Elham GhanbariFertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran0000-0001-6040-1912Ali RezvaniDepartment of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran0000-0002-5011-1302Somayeh GhanbariFertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran0000-0003-2964-2229Mozafar KhazaeiFertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.0000-0003-0536-3217Journal Article20200527<span class="s18"><span class="bumpedFont20"><strong>Background and objectives:</strong></span></span><span class="s4"><span class="bumpedFont20"> Diabetes is associated with a wide range of disorders including oxidative stress. </span></span><span class="s19"><span class="bumpedFont20">Solanum </span></span><span class="s19"><span class="bumpedFont20">melongena</span></span><span class="s4"><span class="bumpedFont20"> </span></span><span class="s19"><span class="bumpedFont20">L.</span></span><span class="s4"><span class="bumpedFont20"> </span></span><span class="s4"><span class="bumpedFont20">possesses</span></span><span class="s4"><span class="bumpedFont20">antioxidant compounds and was introduced as an antidiabetic herb in Asian traditional</span></span><span class="s4"><span class="bumpedFont20"> texts. The aim of the present study was to investigate the effect of </span></span><span class="s4"><span class="bumpedFont20">hydroalcoholic</span></span><span class="s4"><span class="bumpedFont20"> extract of </span></span><span class="s19"><span class="bumpedFont20">S. </span></span><span class="s19"><span class="bumpedFont20">melongena</span></span><span class="s4"><span class="bumpedFont20"> green cap on blood glucose and antioxidant status of liver in diabetic rats. </span></span><strong><span class="s18"><span class="bumpedFont20">Methods:</span></span></strong><span class="s4"><span class="bumpedFont20"><strong> </strong>In this experimental study, </span></span><span class="s4"><span class="bumpedFont20">the extract was prepared by </span></span><span class="s4"><span class="bumpedFont20">maceration </span></span><span class="s4"><span class="bumpedFont20">method and</span></span><span class="s4"><span class="bumpedFont20"> </span></span><span class="s4"><span class="bumpedFont20">42 male </span></span><span class="s4"><span class="bumpedFont20">Wistar</span></span><span class="s4"><span class="bumpedFont20"> rats (190-220 g) were divided into 6 groups (n=7/groups) including: 1. Control; 2. diabetic groups receiving 1 mL distilled water daily; groups 3 and 4 of normal rats receiving 100 and 200 mg/kg extracts, respectively, and diabetic treated groups 5 and 6 with 100 and 200 mg/kg extract, respectively. Diabetes was induced by intraperitoneal injection of </span></span><span class="s4"><span class="bumpedFont20">streptozotocin</span></span><span class="s4"><span class="bumpedFont20"> (55 mg/kg). At the end of study, serum levels of fasting blood glucose (FBG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, nitric oxide (NO) level and total antioxidant capacity (FRAP) were measured. Histological changes of liver were also assessed using hematoxylin and eosin staining. </span></span><strong><span class="s18"><span class="bumpedFont20">Results:</span></span></strong><span class="s4"><span class="bumpedFont20">Treatment of diabetic animals with the extract of </span></span><span class="s19"><span class="bumpedFont20">S. </span></span><span class="s19"><span class="bumpedFont20">melongena</span></span><span class="s19"><span class="bumpedFont20"> </span></span><span class="s4"><span class="bumpedFont20">cap (100 and 200 mg/kg) significantly reduced serum levels of FBG, ALT, ALP, NO (p <0.001) and AST (p=0.017), while total protein (p <0.001) and FRAP (p=0.007) levels increased significantly. The extract also improved the liver tissue changes induced by diabetes. </span></span><strong><span class="s18"><span class="bumpedFont20">Conclusion:</span></span></strong><span class="s19"><span class="bumpedFont20">Solanum </span></span><span class="s19"><span class="bumpedFont20">melongena</span></span><span class="s19"><span class="bumpedFont20"> </span></span><span class="s4"><span class="bumpedFont20">cap is effective in improving liver complications and serum antioxidant status in diabetic rats.</span></span>https://www.rjpharmacognosy.ir/article_113981_32ea2d7bc0311462e8c1fadf2cd7638c.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Molecular Authentication of Radix Behen Albi (“Bahman Sefid”) Commercial Products Reveals Widespread Adulteration576411425410.22127/rjp.2020.226989.1570ENAbdolbaset GhorbaniTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Organismal Biology, Evolutionary Biology Centre, Uppsala University, Norbyvägen, Sweden.0000-0003-1934-9838Mahmoud MosaddeghTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Somayeh Esmaeili*Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0003-1140-0790Hugo De BoerDepartment of Organismal Biology, Evolutionary Biology Centre, Uppsala University, Norbyvägen, Sweden. The Natural History Museum, University of Oslo, Oslo, Norway.Journal Article20200414<strong>Background and objectives: </strong>The roots of <em>Centaurea behen</em> L., (Asteraceae) known as Radix Behen Albi are used as an aphrodisiac, anti-lithiasis and general tonic. It is available as dried or powdered roots in the herbal markets of Iran. Confirming the identity of this medicinal root using conventional methods is challenging because of lack of the diagnostic characters and market samples are easy to misidentify or adulterate. <strong>Methods:</strong> This study aimed to authenticate 13 Radix Behen Albisamples purchased from different herbal markets in Iran and to identify the potential adulterants through DNA barcoding. Nuclear (nrITS) and plastid (<em>trn</em>L-F spacer, <em>mat</em>K and <em>rbc</em>L) DNA regions were used as barcoding markers. A reference database was compiled using sequences from herbarium voucher specimens and publicly available sequences. <strong>Results:</strong> Among used barcode regions nrITS was the best marker for species identification followed by <em>trn</em>L-F spacer. <em>Mat</em>K and <em>rbc</em>L were able to identify samples to the family level. This study showed that none of the market samples belonged to the authentic <em>Centaurea behen</em> L. Sixty-nine percent of samples were <em>Cousinia</em> spp. (Asteraceae), 23% <em>Korshinskya</em> spp. (Apiaceae) and 8% <em>Crambe</em> spp. (Brassicaceae). This substitution does not only hinder consumers obtain the desired medicinal effects of Radix Behen Albi but also raises concerns about the pharmacovigilance of this medicinal root sold in the markets. <strong>Conclusion:</strong> The present study shows the need for monitoring and authentication of crude herbal drugs in the markets of Iran, and that DNA barcoding is a suitable tool for this purpose.https://www.rjpharmacognosy.ir/article_114254_5550d4407392e54b1080ec6c4e426056.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Anti-Anxiety Effects of Artemisia persica in Male Rats657311435510.22127/rjp.2020.232083.1591ENNajmeh AsgharzadehMedical Plants Research Center, Basic Health Sciences Institue, Shahrekord University of Medical Sciences, Shahrekord, Iran.0000-0002-4770-7033Zahra LorigooiniMedical Plants Research Center, Basic Health Sciences Institue, Shahrekord University of Medical Sciences, Shahrekord, Iran.Hossein Amini-KhoeMedical Plants Research Center, Basic Health Sciences Institue, Shahrekord University of Medical Sciences, Shahrekord, Iran.Aazam GhaderianMedical Plants Research Center, Basic Health Sciences Institue, Shahrekord University of Medical Sciences, Shahrekord, Iran.Marzieh MardaniMedical Plants Research Center, Basic Health Sciences Institue, Shahrekord University of Medical Sciences, Shahrekord, Iran.Mohammad-Taghi MoradiMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran0000-0002-3103-3740Mehrdad Shahrani*Medical Plants Research Center, Basic Health Sciences Institue, Shahrekord University of Medical Sciences, Shahrekord, Iran.0000-0003-1284-3569Journal Article20200601<strong>Background and objective</strong>: Anxiety is one of the most common diseases in human societies. Since <em>Artemisia persica </em>has a significant antioxidant capacity with phenolic compounds, and thesesubstances have anti-anxiety effect, the purpose of this research was to determine theAnti-anxiety like effects of Iranian <em>Artemisia persica </em>extract in male rats. <strong>Methods</strong>: <em>Artemisia persica </em>hydroalcoholic extract was prepared by maceration method. Animals were divided into 5 experimental groups. The first group was injected with normal saline. Groups 2 to 4 were injected with <em>Artemisia persica </em>extract at doses of 100, 200 and 400 mg/kg. The fifth group received 2.1 mg/kg of diazepam. The ability to maintain balance of the rates was measured using the rotarod device, anxiety was measured with elevated plus maze, and the motor activity was measured by the open field device. Antioxidant capacity and malondialdehyde levels were also measured in brain and serum tissues. <strong>Results</strong>: Intraperitoneal injection of doses of 100 and 200 mg/kg of the extract increased the number of entering and presence time in the open arm of the plus maze. Doses of 100 and 200 mg/kg of the extract showed significant increase in antioxidant capacity and significant reduction in malondialdehyde levels. In these experiments, the dose of 400 mg/kg showed less effect and in somecases reversed the effects. <strong>Conclusion</strong>: it seems that the anti-anxiety effect of <em>Artemisia persica </em>is dose dependent and increases by increasing the dose to 200 mg/kg; however, at higher dose (400 mg/kg) it shows pro-oxidant effects.<br /> https://www.rjpharmacognosy.ir/article_114355_9758b279087d71f77ead2d37aa8cbd96.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Evaluation of the Cytotoxicity Activity of Gypsophila ruscifolia by Bioassay-Guided Fractionation758211425310.22127/rjp.2020.234735.1606ENMarzie KamaliDepartment of Pharmacognosy, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0002-2989-260XMahmoud Mosaddegh*Department of Pharmacognosy, Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.0000-0001-7737-1158Mohammad-Reza DelnavaziDepartment of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.Roksana ShahrestaniTraditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Maryam Malek MohammadiDepartment of Plant Sciences, School of Biology, College of Sciences, University of Tehran, Tehran, Iran.Maryam Hamzeloo-MoghadamDepartment of Traditional Pharmacy and Traditional Medicine and Materia Medica Research Center, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Journal Article20200617<strong>Background and objectives:</strong> The second cause of death in the world and the third cause in Iran is cancer which requires special attention for treatment. The previous reports of <em>Gypsophila</em> genus show significant toxic effects on different cancer cell lines. In this study, bioassay-guided fractionation was applied to determine the cytotoxic activity of root and aerial parts extracts and fractions of <em>Gypsophila ruscifolia</em> <strong>Methods:</strong> n-Hexane, chloroform, and methanol: H2O (8:2) extracts of root and aerial parts were prepared. Inhibition of cell growth determined by MTT assay in MCF-7, HT-29, A-549, and AGO-1522 cell lines. The most effective extract was fractionated by column chromatography. The cytotoxic effect of fractions was evaluated by MTT assay and apoptotic property of the cytotoxic fraction was determined by annexin V/PI assay in MCF-7 cell line. <strong>Results:</strong> The chloroform root extract of <em>G. ruscifolia</em> showed cytotoxicity in MCF-7 cells with IC<sub>50</sub> value of 111.6 ±11.78 μg/mL. MTT assay of five of fractions demonstrated that F3 and F4 with IC<sub>50</sub> values of 73.09 ±14.22 and 67.98 ±15.31 μg/mL on MCF-7 cell line, respectively showed cytotoxic effects. Also, F4 demonstrated apoptotic potential in MCF-7 cell line. <strong>Conclusion:</strong> Considering the results of cytotoxicity and apoptosis studies, isolation and identification of responsible compounds in the chloroform root extract of <em>Gypsophila ruscifolia</em> can be useful in cancer research studies.https://www.rjpharmacognosy.ir/article_114253_80d9e7168566eb08b8cc34fa88f58d6a.pdf- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical SciencesResearch Journal of Pharmacognosy2345-44587420201001Influence of Sex on Food Intake and Body Weight in Wistar Rats in a Repeated Administration of Bridelia ferruginea Benth Aqueous Extract839111483010.22127/rjp.2020.231427.1588ENSemi Anthelme Nene-Bi*Laboratory of Animal Physiology, UFR Biosciences, Felix Houphouët-Boigny University, Abidjan, Ivory Coast.0000-0003-3162-2075Ramachandran VadivelanDepartment of Pharmacology, JSS College of Pharmacy (JSS Academy Higher Education and Research), Ooty, Tamil Nadu, India.Ouga Stanislas ZahouiLaboratory of Animal Physiology, UFR Biosciences, Felix Houphouët-Boigny University, Abidjan, Ivory Coast.Palanisamy DhanabalDepartment of Pharmacognosy and Phytopharmacy, JSS College of Pharmacy (JSS Academy Higher Education and Research), Ooty, Tamil Nadu, India.Flavien TraoreLaboratory of Animal Physiology, UFR Biosciences, Felix Houphouët-Boigny University, Abidjan, Ivory Coast.Journal Article20200519<strong>Background and objectives: </strong><em>Bridelia ferruginea</em> Benth , a medicinal plant widely used in traditional medicine in Ivory Coast to treat many pathologies such as diabetes mellitus, is usually taken during a short or long period and its bark used as vegetables contains anti-nutritional factors.. The aim of the present study was to assess the effects of the plant extract on food consumption and body weight in Wistar female and male rats.<strong> Methods: </strong>The aqueous extract was prepared using the powder (100 g) of the stem bark of <em>Bridelia ferruginea</em> macerated in distilled water (1 Liter) during 24 hours. For the present study, thirty-two rats were randomly divided into four groups of 8 animals each (8; 4/sex). The experiment was performed in 90 days, according the protocol described by the Organization for Economic Cooperation and Development guideline 408 for testing chemicals in sub chronic toxicity study in rodents. Food consumption and animal body weight were measured each day during the experiment period<em>.</em><strong> Results: </strong>The food consumption decreased in female group compared to the male rats receiving the same treatment (p <0.05). During three months of measurement, decrease of food consumption was observed in all female rat groups (control and treated, p <0.001) compared to male groups having the same treatment. Weekly measures of body weight showed decrease in female rats compared to male groups receiving the same dose.<strong> Conclusion: </strong><em>Bridelia ferruginea</em> caused lower body weight and food consumption in female rats compared to the male that suggested female Wistar rats were more sensitive than male.https://www.rjpharmacognosy.ir/article_114830_6bc15714e7add8fbdc01f4a22786e1ed.pdf