TY - JOUR ID - 52974 TI - Isolation and characterization of acetylcholinesterase inhibitors from Piper longum L. JO - Research Journal of Pharmacognosy JA - RJP LA - en SN - 2345-4458 AU - Khatami*, Z. AU - Sarkhail, P. AU - Adhami, H.R. AD - Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. AD - Pharmaceutical Sciences Research Center, Tehran University of Medical Science, Tehran, Iran. Y1 - 2017 PY - 2017 VL - 4 IS - Supplement SP - 2 EP - 2 KW - AChE inhibitor KW - Alzheimer’s disease KW - Ellman’s assay KW - Piper longum Linn KW - TLC bioautography DO - N2 - Background and objectives: The cholinergic deficit correlates with the severity of Alzheimer’s disease. The cholinergic function can be improved by AChE inhibitors blocking this key enzyme in the breakdown of acetylcholine. Based on traditional medicine, during two last decades the use of herbal medicinal substances in dementia therapy has been studied. The fruit extract of Piper longum L. (Piperaceae) has been used in traditional medicine with various therapeutic properties, among those for the enhancement of cognitive performance in several herbal mixtures. It has been demonstrated that the fruit extract of P. Longum inhibites AChE. In order to find new natural compounds with AChE inhibitory effect, in this study the fruits of P. longum were investigated. Methods: First the fruits were pulverised smoothly, and then extracted with dichloromethane by stirring after sonification. The extract was investigated by a enzymatic TLC bioautography assay to identify the active zones. Then the active compounds were isolated using several chromatographic techniques. Results: The structures of active components were characterized by different methods like 1H, 13C NMR-spectroscopy, FTIR and mass-spectrometry. The four isolated substances were identified as methylen-dioxide and piperine derivatives. Conclusion: The present study indicated the reasonability of the use of this plant in traditional medicine for treatment of cognitive performance. UR - https://www.rjpharmacognosy.ir/article_52974.html L1 - https://www.rjpharmacognosy.ir/article_52974_5e145fa9bae7fbd63d7c4c6fb627ffc1.pdf ER -