%0 Journal Article %T Silibinin upregulates E-cadherin expression in MKN-45 human gastric cancer cells %J Research Journal of Pharmacognosy %I - The Iranian Society of Pharmacognosy - Shahid Beheshti University of Medical Sciences %Z 2345-4458 %A Faghihloo*, Ebrahim %A Mirzaei, Habibollah %A Akbari, Abolfazl %A Goudarzi, Hossein %D 2018 %\ 07/01/2018 %V 5 %N 3 %P 41-46 %! Silibinin upregulates E-cadherin expression in MKN-45 human gastric cancer cells %K E-cadherin %K Gastric cancer %K MKN-45 %K silibinin %R 10.22127/rjp.2018.64874 %X Background and objectives:  Gastric cancer is currently known as one of the most important causes of cancer-driven death all over the world. In patients with gastric cancer, a significant proportion of death occurs due to metastasis. On the other hand, down modulated E-cadherin level has been reported as an important contributor to tumor cell invasion and metastasis. In this regard, the present work was aimed to evaluate the impact of silibinin, a flavonolignan with established anti-tumor efficacy, on cell viability and E-cadherin expression in the gastric cancer cell line MKN-45. Methods: To determine cell viability, MTT assay was performed 48 h after silibinin treatment (at concentrations of 100, 200 and 400 μM). In addition, quantitative real-time PCR was done following total RNA extraction and cDNA synthesis, to assess E-cadherin level in cells treated with silibinin. Results: The MTT results showed concentration-dependent reducing effect of silibinin on viability of MKN-45 cells. The findings of quantitative real-time PCR analysis demonstrated upregulated E-cadherin expression in cells treated with silibinin (significantly (p≤ 0.05) at concentration of 200 μM) compared to the control cells. Conclusions: The current study suggested that silibinin may exert anti-migratory/invasive effects on gastric cancer cells by enhancing E-cadherin expression, which needs to be further investigated.   %U https://www.rjpharmacognosy.ir/article_64874_255a41e4dff91aae430ce6eeff01b12a.pdf