%0 Journal Article %T Holothurin B Isolated from Holothuria atra Inhibits Angiogenesis More Potent than Curcumin in Vitro %J Research Journal of Pharmacognosy %I - The Iranian Society of Pharmacognosy - Shahid Beheshti University of Medical Sciences %Z 2345-4458 %A Yegdaneh, Afsaneh %A Safaeian*, Leila %A Mirian, Mina %A Dana, Nasim %A Taheri, Mohadeseh %D 2021 %\ 07/01/2021 %V 8 %N 3 %P 33-40 %! Holothurin B Isolated from Holothuria atra Inhibits Angiogenesis More Potent than Curcumin in Vitro %K angiogenesis inhibitors %K cell migration inhibition %K Cell proliferation %K holothurin B %K human umbilical vein endothelial cells %R 10.22127/rjp.2021.277328.1687 %X Background and objectives: Triterpene glycosides as the most bioactive components of sea cucumbers, have been considered for their various pharmacological properties especially anticancer and anti-metastasis activities. Due to the limited information on the biological properties of holothurin B as a marine triterpene glycoside, the present study aimed to examine its effect on angiogenesis and compare it with curcumin usinghuman umbilical vein endothelial cells (HUVECs). Methods: Holothurin B was isolated from Holothuria atra and identified by NMR and Mass spectroscopic data. Cell survival was estimated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique and migration of cells was assessed by Transwell test. Angiogenesis was evaluatedin vitro by tube formation assay. Results: Holothurin B reduced HUVECssurvival with IC50 value of 8.16 µg/mL. At the concentrations of 5 and 7.5 µg/mL, it significantly decreased the number of migrated cells, the average length and size of tubules, and mean number of junctions; it was more potent than curcumin. Conclusion: Holothurin B could be considered as a potent antiangiogenic constituent through suppressing endothelial cell proliferation, migration and tubulogenesis in vitro, suggesting its potential for further animal and clinical investigations.  %U https://www.rjpharmacognosy.ir/article_130034_295f1fe86421bcce0f9aab21a2117316.pdf