@article { author = {Asghari, G. and Nasr Esfahani, B. and Paydar, P.}, title = {Evaluating the effect of Dracocephalum kotschyi methanol extract on Mycobacterium tuberculosis}, journal = {Research Journal of Pharmacognosy}, volume = {2}, number = {3}, pages = {31-36}, year = {2015}, publisher = {- The Iranian Society of Pharmacognosy - Shahid Beheshti University of Medical Sciences}, issn = {2345-4458}, eissn = {2345-5977}, doi = {}, abstract = {Background and objectives: Tuberculosis (TB) is the major public health problem in the world. Each year there are 2-3 million deaths worldwide caused by TB. The increasing incidence of Multi Drug Resistance tuberculosis (MDR-TB) worldwide highlights the urgent need to search for new anti-tuberculosis compounds. It has been reported that medicinal plant, Dracocephalum kotschyi, possesses some antibacterial effect, thus in the present study its anti-mycobacterial property was evaluated. Methods: The sensitivity and resistance of M. tuberculosis strains at concentration of 0.2 µg/mL isoniazid was determined by proportion method. Methanol extract of D. kotschyi was prepared using maceration method. Six concentrations of D. kotschyi, including 20, 40, 80, 160, 320 and 640μg/mL were prepared and its anti-mycobacterial effect on four groups of M. tuberculosis including M. tuberculosis H37Rv (ATCC 27294), isoniazid susceptible and resistance and MDR strains was determined. Results: The methanol extract of D. kotschyi was significantly against M. tuberculosis. The percent of growthwas decreased from 100% to 0% in M. tuberculosis  H37Rv (ATCC 27294),  isoniazid resistant and isoniazid susceptible strains but from 100% to 50% in MDR strain in 640 μg/mL concentration. Conclusion: The results showed that different concentrations of D. kotschyi methanol extracts showed a remarkable inhibitory effect on M. tuberculosis. Identification of the effective fraction of D. kotschyi against M. tuberculosis is a further step to be studied.}, keywords = {Dracocephalum kotschyi,Isoniazid,Methanol extract,Mycobacterium Tuberculosis}, url = {https://www.rjpharmacognosy.ir/article_9951.html}, eprint = {https://www.rjpharmacognosy.ir/article_9951_b330e33a91ef067301479c9f64b73233.pdf} }