@article { author = {Tofighi, Z. and Ostad, S.N. and Khezrrahdoost, S. and Salehizadeh, H. and Yassa, N.}, title = {Potent anti-nociceptive and anti-inflammatory effects of methanol fraction of Otostegia persica extract and its components}, journal = {Research Journal of Pharmacognosy}, volume = {4}, number = {2}, pages = {23-29}, year = {2017}, publisher = {- The Iranian Society of Pharmacognosy - Shahid Beheshti University of Medical Sciences}, issn = {2345-4458}, eissn = {2345-5977}, doi = {}, abstract = {Background and objectives: Otostegia persica (Labiatae) is an endemic plant of Iran and is used for its anti-inflammatory properties in folk medicine of Sistan and Baluchestan province. The aim of the present study was to investigate the anti-nociceptive and anti-inflammatory effects of O. Persica different fractions and identification of the natural compounds from the most active fraction. Methods: Total extract of O. Persica was fractionated with petroleum ether (PE), chloroform (CL), ethyl acetate (EA), n-butanol (BU) and methanol (ME). The analgesic activities of different fractions were determined by formalin test. Then, activity of effective fractions was investigated on carrageenan-induced paw edema assay. Finally, the compounds of effective fraction were isolated and their structures were elucidated. Results: Anti-nociceptive activity of EA and BU fractions (100 mg/kg) and ME fraction (100 and 200 mg/kg) demonstrated significant difference with normal saline during the second phase of the formalin test. ME fraction showed higher analgesic effects in comparison to indomethacin (p<0.05), with IC50 equal to 85.87 mg/kg. Among EA, BU and ME fractions which were selected for anti-inflammatory investigation, EA could not reduce rat paw edema after 6 h. The swelling inhibition percentage of ME was similar to that induced by indomethacin at the same time (p>0.05). Vicenin-2 and isorhamnetin-3-O-glucoside were elucidated from ME as the effective anti-inflammatory fraction. Conclusion: It was concluded that the existence of flavonoids in O. persica extract could play an important role for its anti-nociceptive and anti-inflammatory effects similar to various non-steroidal anti-inflammatory drugs (NSAIDS) and inhibitors of nitric oxide synthase (NOS).}, keywords = {carrageenan,Flavonoids,Formalin test,Labiatae,Otostegia persica}, url = {https://www.rjpharmacognosy.ir/article_44250.html}, eprint = {https://www.rjpharmacognosy.ir/article_44250_fb74e911fa2e634be5592b1567c83e99.pdf} }