Cytotoxicity, antioxidant activity, total flavonoid and phenolic contents of Salvia urmiensis Bunge and Salvia hydrangea DC. ex Benth.

Document Type : Original paper

Authors

1 Department of Chemistry, Faculty of Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran

2 Department of Chemistry, Miyaneh Branch, Islamic Azad University, Miyaneh, Iran.

Abstract

Background and objectives: Salvia species are important because of their medicinal, traditional and economical uses. They are used traditionally for treatment of several diseases. The genus Salvia is represented in the Iranian flora by 61 species of which, 17 are endemic.  In the present study, the phytochemical and biological effects of two Iranian Salvia species have been evaluated. Methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals scavenging activities of extracts of Salvia urmiensis Bunge and Salvia hydrangeawere evaluated. Total flavonoid and phenolic contents and brine shrimp lethality potential of the extracts were also determined. Results: Compared to podophyllotoxin (LC50 =42 µg/mL), the ethyl acetate extract of S. hydrangea demonstrated a significant cytotoxicity (LC50=36 µg/mL). The ethyl acetate extract of S. urmiensis was found to have significant antioxidant properties with IC50 value of 10.0±0.2 µg/mL. All tested extracts showed moderate to high flavonoid and phenolic contents. Conclusion: Findings showed that these plants contain important metabolites and could be suggested for discovery of biologically active natural compounds.

Keywords

References

[1] Valizadeh H, Mahmoodi FK, Kouhkan R, Bahadori MB. Isolation and structure elucidation of coumarin and cinamate derivatives from Lycium ruthenicum. Iran Chem Commun. 2014; 2: 277-282.
[2] Valizadeh H, Mahmoodi FK, Alizadeh Z, Bahadori MB. Isolation and structure elucidation of secondary metabolites from Echinophora platyloba DC from Iran. J Med Plant. 2014; 13: 15-21.
[3] Valizadeh H, Mahmoodi FK, Hosseinzadeh Z, Bahadori MB. Furanocoumarins from Heracleum rawianum in Iran. Iran Chem Commun. 2015; 3: 1-6.
[4] Sonboli A, Bahadori MB, Dehghan H, Aarabi L, Savehdoroudi P, Nekuei M, Pournaghi N, Mirzania F. Chemotaxonomic importance of the essential oil composition in two subspecies of Teucrium stocksianum Boiss. from Iran. Chem Biodivers. 2013; 10: 687-694.
[5] Wu YB, Ni ZY, Shi QW, Dong M, Kiyota H, Gu YC, Cong B. Constituents from Salvia species and their biological activities. Chem Rev. 2012; 112: 5967-6026.
[6] Topcu G. Bioactive triterpenoids from Salvia Species. J Nat Prod. 2006; 69: 482-487.
[7] Ulubelen A. Cardioactive and antibacterial terpenoids from some Salvia species. Phytochemistry. 2003; 64: 395-399.
[8] Imanshahidi M, Hosseinzadeh H. The pharmacological effects of Salvia species on the central nervous system. Phytother Res. 2006; 20: 427-437.
[9] Kamatou GPP, Makunga NP, Ramogola WPN, Viljoen AM. South African Salvia species: a review of biological activities and phytochemistry. J Ethnopharmacol. 2008; 119: 664-672.
[10] Shirota O, Nagamatsu K, Sekita S. Neo-clerodane diterpenes from the hallucinogenic Sage Salvia divinorum. J Nat Prod. 2006; 69: 1782-1786.
[11] Lu Y, Foo LY. Polyphenolics of Salvia-a review. Phytochemistry. 2002; 59: 117-140.
[12] Jamzad Z.  Flora of Iran: Lamiaceae. 1st ed. Tehran: Research Institute of Forests and Rangelands press, 2012.
[13] Farimani MM, Ebrahimi SN, Salehi P, Bahadori MB, Sonboli A, Khavasi HR, Zimmermann S, Kaiser M, Hamburger M. Antitrypanosomal triterpenoid with an ε‑Lactone E‑Ring from Salvia urmiensis. J Nat Prod. 2013; 76: 1806-1809.
[14] Farimani MM, Bahadori MB, Taheri S, Ebrahimi SN, Zimmermann S, Brun R, Amin G, Hamburger M. Triterpenoids with rare carbon skeletons from Salvia hydrangea: antiprotozoal activity and absolute configurations. J Nat Prod. 2011; 74: 2200-2205.
[15] Farimani MM, Taheri S, Ebrahimi SN,  Bahadori MB, Khavasi HR, Zimmermann S, Brun R, Hamburger M. Hydrangenone, a new isoprenoid with an unprecedented skeleton from Salvia hydrangea. Org Lett. 2012; 14: 166-169.
[16] Xu X, Xie H, Wang Y, Wei X. A-Type proanthocyanidins from lychee seeds and their antioxidant and antiviral activities. J Agric Food Chem. 2010; 58: 11667-11672.
[17] Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice EC. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999; 26: 1231-1237.
[18] Meyer BN, Ferrigni NR, Putnam JE, Jacobsen LB, Nichols De, McLaughlin Jl. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med. 1982; 45: 31-34.
[19] Zhou K, Su L, Yu L. Phytochemicals and antioxidant properties in wheat barn. J Agric Food Chem. 2004; 52: 6108-6114.
[20] Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999; 64: 555-559.
[21] Firuzi O, Miri R, Asadollahi M, Eslami S, Jassbi AR. Cytotoxic, antioxidant and antimicrobial activities and phenolic contents of eleven Salvia species from Iran. Iran J Pharm Res. 2013; 12: 801-810.
[22] Pan ZH, Wang YY, Li MM, Xu G, Peng LY, He J, Zhao Y, Li Y, Zhao QS. Triterpenoids from Salvia trijuga. J Nat Prod. 2010; 73: 1146-1150.
[23] Hussein AA, Meyer JJM, Jimeno JM, Rodriguez B. Bioactive diterpenoids from Orthosiphon labiatus and Salvia Africana-lutea. J Nat Prod. 2007; 70: 293-295.

Files

Share

How to cite

Statistics