Protective Effects of Curcumin on the Structural Parameters of Seminiferous Tubules and Leydig Cells in Methandienone Treated Mice

Document Type : Original paper

Authors

Department of Basic and Pathobiological Sciences, Faculty of Veterinary Medicine, Razi Universtiy, Kermanshah, Iran.

Abstract

Background and objectives: Although anabolic androgenic steroids (AAS) use has become became more prominent in bodybuilders and professional athletes, their consumption is nowadays a problem that involves a wider population. Methandienone or dianabol is an anabolic androgenic steroid which is used by athletes for fitness. This study was conducted to investigate, the likely protective effects of curcumin on the structural parameters of the testis in dianabol treated mice. Methods: A total of 35 male mice were divided into five groups, each containing seven mice. The control group received 0.2 mL distilled water; the second group received 20 mg/kg/day dianabol; third, fourth, and fifth groups received dianabol plus 50, 100, and 200 mg/kg/day of curcumin, respectively. All administrations were done by gavages for 56 days. On the last day, the left testes were examined using stereological methods. The volume of testis and seminiferous tubules, interstitial tissue, and diameter, length and germinal epithelium height of the tubules as well as the number of Leydig cells were estimated. Results:  The obtained results showed that the weight and volume of the testis as well as volume of the seminiferous tubules and interstitial tissue, length, diameter and epithelium height of the tubules decreased significantly (p<0.05) after dianabol administration compared to the negative control group. Oral administration of curcumin at doses 100 and 200 mg/kg, could significantly improve all the measured parameters toward the normal levels. Conclusion: It seems that curcumin due to its anti-oxidant and anti-inflammatory properties can inhibit testis volume reduction and hypogonadism following dianabol administration. 

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References

[1] Foss GL, Simpson SL. Oral methyltestosterone and jaundice. Br Med J. 1959; 1(5117): 259-263.
[2] Foss GL. Some experiences with a new anabolic steroid (methandrostenolone). Br Med J. 1960; 1(5182): 1300-1305.
[3] Moss JL, Crosnoe LE, Kim ED. Effect of rejuvenation hormones on spermatogenesis. Fertil Steril. 2013; 99(7): 1814-1820.
[4] Shahidi NT. A review of the chemistry, biological action, and clinical applications of anabolic- androgenic steroids. Clin Ther.2001; 23(9): 1355-1390.
[5] Shahidi NT, Diamond LK. Testosterone-induced remission in aplastic anemia of both acquired and congenital types. Further observations in 24 cases. N Engl J Med. 1961; 264(19): 953-967.
[6] Coward RM, Rajanahally S, Kovac JR, Smith RP, Pastuszak AW, Lipshultz LI. Anabolic  steroid induced hypogonadism in young men. J Urol. 2013; 190(6): 2200-2205.
[7] Hartgens F, Kuipers H. Effects of androgenic-anabolic steroids in athletes. Sports Med. 2004; 34(8): 513-554.
[8] Fronczak CM, Kim ED, Barqawi AB. The insults of illicit drug use on male fertility. J Androl. 2012; 33(4): 515-528.
[9] Garevik N, Strahm E, Garle M, Lundmark J, Stahle L, Ekstrom L, Ekstrom L, Rane A. Long term perturbation of endocrine parameters and cholesterol metabolism after discontinued abuse of anabolic androgenic steroids. J Steroid Biochem Mol Biol. 2011; 127(3-5): 295-300.
[10] Boregowda K, Joels L, Stephens JW, Price DE. Persistent primary hypogonadism associated with anabolic steroid abuse. Fertil Steril. 2011; 96(1): 7-8.
[11] Parkinson AB, Evans NA. Anabolic androgenic steroids: a survey of 500 users. Med Sci Sports Exerc. 2006; 38(4): 644-651.
[12] Glusker J. Intermolecular interactions around functional groups in crystals: data for modeling the binding of drugs to biological macromolecules. Acta Crystallogr. 1995; 51(4): 418-427.
[13] Agarwal A, Nallella K, Allamaneni S, Said T. Role of antioxidants in treatment of male infertility: an overview of the literature. Reprod Biomed Online. 2008; 8(6): 616-627.
[14] Daniel S, Limson J, Dairam A, Watkins G, Daya S. Through metal binding curcumin protects against lead-and cadmium induced lipid peroxidation in rat brain homogenates and against lead-induced tissue damage in rat brain. J Inorg Biochem. 2004; 98(2): 266-275.
[15] Jungil H, Mousumi B, Jihyeuny J, Jae-Ha R, Xiaoxin C, Shengmin S. Modulation of arachidonic acid metabolism by curcumin and related B-diketone derivatives: effect on cytosolic phospholipase A, cyclooxygenases and 5- lipoxygenase. Carcinogenesis. 2004; 25(9): 1671-1679.
[16] Ilbey YO, Ozbek E, Cekmen M, Simsek A, Otunctemur A, Somay A. Protective effect of curcumin in cisplatin-induced oxidative injury in rat testis: mitogen-activated protein kinase and nuclear factor-kappa B signaling pathways. Hum Reprod. 2009; 24(7): 1717-1725.
[17] Mathuiria N, Verma RJ. Curcumin ameliorates aflatoxin-induced lipid peroxidation in liver, kidney and testis of mice – an in vitro study. Acta Pol Pharm. 2007; 64(5): 413-416.
[18] El-Demerdash FM, Yousef MI, Radwan FM. Ameliorating effect of curcumin on sodium arsenite-induced oxidative damage and lipid peroxidation in different rat organs. Food Chem Toxicol. 2009; 47(1): 249-254.
[19] Karbalay-Doust S, Noorafshan A. Ameliorative effects of curcumin on the spermatozoon tail length, count, motility and testosterone serum level in metronidazole-treated mice. Prague Med Report. 2011; 112(4): 288-297.
[20] Silva MAJ, Merzel J. Stereological determination of the volume of the rat hemimandible rat tissue. Anat Rec. 2001; 263(3): 255-259.
[21] Gundersen  HJ, Bendtsen TF, Korbo L, Marcussen N, Moller A, Nielsen K. Some new, simple   and efficient stereological methods and their use in pathological research and diagnosis. J Pathol Microbiol Immunol. 1988; 96(5): 379-394.
[22] Nyengaard JR. Stereologic methods and their application in kidney research. J Am Soc Nephrol. 1999; 10(5): 1100-1123.
[23] Mandarim-de-Lacerda CA. Stereological tools in biomedical research. An Acad Bras Cienc. 2003; 75(4): 469-486.
[24] Sterio DC. The unbiased estimation of number and sizes of arbitrary particles using the     dissector. J Microsc. 1984; 134(2): 127-136.
[25] Mazhar Hijazi M, Ahmed Azmi M, Hussain A, Naqvi SNH, Perveen R, Hijazi S. Androgenic anabolic steroidal-based effects on the morphology of testicular structures of albino rats. Pak J Zool. 2012; 44(6): 1529-1537.
[26] Pope HGJR, Katze DL. Psychiatric and medical effects of anabolic-androgenic steroids use. A controlled study of 160 athletes. Arch Gen Psychiat. 1994; 51(5): 375-382.
[27] Smith DA, Perry PJ. The efficacy of ergogenic agents in athletic competition. Part I: Androgenic-anabolic steroids. Ann Pharmacother. 1992; 26(4): 520-528.
[28] Karbalay-Doust S, Noorafshan A. Stereological study of the effects of nandrolone decanoate on the rat prostate. Micron. 2007; 37(7): 617-623.
[29] Mutalip SSM, Singh SGK, Shah MA, Mohamad M, Mani V, Hussin SN. Histological changes in testes of rats treated with testosterone, nandrolone, and stanozolol. Iran J Reprod Med. 2013; 11(8): 653-658.
[30] Noorafshan A, Karbalay-Doust S, Mesnah-Ardekani F. High doses of nandrolone decanoate reduces volume of testis and length of seminiferous tubules in rats. Revise. 2005; 113(2): 122-125.
[31] Feinberg MJ, Lumia AR, McGinnis MY. The effect of anabolic-androgenic steroids on sexual behavior and reproductive tissues in male rats. Physiol Behav. 1997; 62(1): 23-30.
[32] Dohle GR, Smit M, Weber RF. Androgens and male fertility. World J Urol. 2003; 21(5): 341-345.
[33] Adnan MJ, Alzamely AN, Hamad AG.  Study of the testicular damage induced by dianabol and its effect on morphological and histological changes in albino male rats. J Agri Vet Sci. 2015; 8(8): 24-32.
[34] Khder HR, Falah MA. The effect of sustanon (testosterone derivatives) taken by athletes on the testis of rat. Jordan J Biol Sci 2012; 5(2): 113-119.
[35] Zirkin BR, Santulli R, Awoniyi CA, Ewing LL. Maintenance of advanced spermatogenic cells in the adult rat testis: quantitative relationship to testosterone concentration within the testis. Endocrinol. 1989; 124(6): 3043-3049.
[36] Alonso-Alvarez C, Bertrand S, Faivre B, Chastel O, Sorci G. Testosterone and oxidative stress: the oxidation handicap hypothesis. Proc Biol Sci. 2007; 274(1611): 819-825.
[37] Mei X, Xu D, Xu S, Zheng Y, Xu S. Novel role of Zn(II)-curcumin in enhancing cell proliferation and adjusting proinflammatory cytokine-mediated oxidative damage of ethanol-induced acute gastric ulcers. Chem  Biol  Interact. 2012; 197(1): 31-39.
[38] Ma C, Ma Z, Fu Q, Ma S. Curcumin attenuates allergic airway inflammation by regulation of CD4+CD25+ regulatory T cells (Tregs)/Th17 balance in ovalbumin-sensitized mice. Fitoterapia. 2013; 87: 57-64.
[39] Kanitkar M, Gokhale K, Galande S, Bhonde RR. Novel role of curcumin in the prevention of cytokine-induced islet death in vitro and diabetogenesis in vivo. Br J Pharmacol. 2008; 155(5): 702-713.
[40] Cole GM, Teter B, Frautschy SA. Neuroprotective effects of curcumin. Adv Exp Med Biol.  2007; 595: 197-212.
[41] Khorsandi L, Mirhoseini M, Mohamadpour M, Orazizadeh M, Khaghani S. Effect of curcumin on dexamethasone-induced testicular toxicity in mice. Pharm Biol. 2013; 51(2): 206-212.
[42] Giannessi F, Giambelluca MA, Grasso L, Scavuzzo MC, Ruffoli R. Curcumin protects Leydig cells of mice from damage induced by chronic alcohol administration. Med Sci Monit. 2008; 14(11): 237-242.
Research Journal of Pharmacognosy
Volume 5, Issue 4
October 2018
Pages 33-41

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