Study of wound healing potential of Stevia rebaudiana ethanol extract in male rats

Document Type: Original paper

Authors

1 Department of Toxicology, Faculty of Veterinary Medicine, Tehran, Iran.

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.

3 Department of Microbiology Section, Pathobiology & Basic Sciences, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.

4 Department of Chemistry, Payame Noor University, Tehran, Iran.

5 Department of Dermatology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.

6 Research Pharmaceutical Center, School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

Abstract

Background and objectives: Stevia rebaudiana has been used in medicine as anti-inflammatory, antioxidant, antipyretic, anti-fungal, and antibacterial agent. The present study was conducted to investigate the healing effects of S. rebaudiana ethanol extracts on cutaneous wounds in rats. Methods: Full-thickness excisional wounds (2×2 cm) were induced on the back of 32 rats. The rats were divided into four groups as follows; untreated (control) and treated with 1 mL basal cream, 1 mL S. rebaudiana ethanol extract 10%, and 1 mL tetracycline (3%) for 20 days (short term). Animals of each group were euthanized at 20 day post-injury and wounds were assessed through macroscopic and microscopic analyses. Results: During the experiment, S. rebaudiana indicated a significant reduction in the wound area compared to other groups. Parameters such as arrangement of the healing tissue, re-epithelilization and epithelial formation demonstrated considerable changes when compared to the control. In addition, treatment with S. rebaudiana decreased the total number of cells, fibrocytes/fibroblasts ratio, neutrophils, and lymphocytes and enhanced the number of blood vessels and fibroblasts at 20 day. Conclusion: The present study demonstrated the wound healing activity of S. rebaudiana, lending credence to the folkloric use in the treatment of cutaneous wounds.

Keywords


[1] Faramarzi E, Zangeneh MM, Zangeneh A, Moradi R. Effect of Cinnamomum zelanicumon oil on hyponeophagia anxiety test in Balb C male mice. Online J Vet Res.2017; 21(2): 77-80.

[2] Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R. Medicinal plants: antibacterial effects and chemical composition of essential oil of Foeniculum vulgare. Int J Curr Pharm Rev Res.2017; 8(1): 13-17.

[3] Foroughi A, Pournaghi P, Najafi F, Zangeneh A, Zangeneh MM, Moradi R. Antibacterial effect and phytochemical screening of essential oil of Pimpinella anisum against Escherichia coli O157:H7 and Staphylococcus aureus. Int J Curr Med Pharm Res. 2016; 7(6): 367-371.

[4] Foroughi A, Zangeneh MM, Kazemi N, Zangeneh A. An in vitro study on antimicrobial properties of Allium noeanumreut ex regel: an ethnomedicinal plant. Iran J Publ Health. 2016; 45(2): 32.

[5] Hagh-Nazari L, Goodarzi N, Zangeneh MM, Zamgeneh A, Tahvilin R, Moradi R. Stereological study of kidney in streptozotocin-induced diabetic mice treated with ethanolic extract of Stevia rebaudiana (bitter fraction). Comp Clin Pathol. 2017; 26 (2): 455-463.

[6] Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Tahvilian R, Hidaryan H, Rezaeeas N, Kohneshin A. Antibacterial properties of an Iranian ethnomedicinal plant. Int J Ayu Pharm Chem.2017; 6(3): 128-137. 

[7] Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Faizi S, Zoalfaghari M, Marabi A. Study a plant extract as an antibacterial agent. Int J Curr Med Pharm Res. 2017; 3(2): 1360-1362.

[8] Zangeneh MM, Najafi F, Tahvilian R, Haghnazari L, Zangeneh A, Moradi R, Mahmoudifar A. Effect of Scrophularia striata hydro-alcoholic extract on growth of Bacillus subtilis ATCC No. 21332. Online J Vet Res. 2017; 21(2): 51-57.

[9] Foroughi A, Zangeneh MM, Zangeneh A, Kazemi N. A survey on antibacterial activities of Allium eriophyllum alcoholic extract: an ethnomedicinal plant. Iran J Publ Health. 2016; 45(2): 32.

[10] Zangeneh MM, Tahvilian R, Najafi F, Zangeneh A, Souri N, Moeini-Arya M, Zhaleh S. Evaluation of the in vitro antibacterial effect of the hydroalcoholic extract of Scrophularia striata. Int J Sci Eng Res. 2016; 7(10): 1693-1702.   

[11] Sherkatolabbasieh H, Hagh-Nazari L, Shafiezadeh S, Goodarzi N, Zangeneh MM, Zangeneh A. Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: a stereological examination. Arch Biol Sci.2017; 69(3): 535-543.

[12] Hosamani KM, Ganjihal SS, Chavadi DV. Alternanthera triandra seed oil: a moderate source of ricinoleic acid and its possible industrial utilization. Indus Crop Prod. 2004; 19(2): 133-136.

[13] Schultz GS. Molecular regulation of wound healing. In: acute and chronic wounds: nursing management. Bryant R.A. (Ed.). 2nd ed. WB Saunders ,1999.

[14] Nagori BP, Solanki R. Role of medicinal plants in wound healing. Res J Med Plants. 2011; 5(4): 392-405.

[15] Zangeneh MM, Najafi F, Tahvilian R, Haghnazari L, Zangeneh A, Abiari M, Moradi R. Study on the in vitro antibacterial properties of alcoholic extract of Stevia rebaudiana in west of Iran. Int J Sci Eng Res. 2016; 7(11): 1352-1359.

[16] Zangeneh MM, Najafi F, Moradi R, Tahvilian R, Haghnazari L, Zangeneh A. Evaluation of the in vitro antibacterial activities of alcoholic extract of Stevia rebaudiana against Escheriachia coli O157: H7 (ATCC No. 25922). Asian J Pharm Anal Med Chem. 2016; 4(3): 131-136. 

[17] Zangeneh MM, Poyanmehr M, Najafi F, Zangeneh A, Moradi R, Tahvilian R, Haghnazari L. In vitro antibacterial activities of ethanolic extract of Stevia rebaudiana against Bacillus subtilis (ATCC No. 21332). IntJResPharma Nano Sci.2016; 5(6): 320-325.

[18] Zangeneh MM, Najafi F, Tahvilian R, Salmani S, Haghnazari L, Zangeneh A, Moradi R. Ethnomedicinal plants: in vitro antibacterial effects of ethanolic extract of Stevia rebaudiana. Int J Ayu Pharm Chem. 2017; 6(1): 251-259.

[19] Oryan A, Tabatabaieinaieni A, Moshiri A, Mohammadalipoor A, Tabandeh MR. Modulation of cutaneous wound healing by silymarin in rats. J Wound Care. 2012; 21(9): 457-464.

[20] Oryan A, Mohammadalipour A, Moshiri A, Mohammad R, Tabandeh MR. Avocado/soybean unsaponifiables: a novel regulator of cutaneous wound healing, modelling and remodeling. Int Wound J. 2015; 12(6): 674-685.

[21] Chitra S, Patil MB, Ravi K. Wound healing activity of Hyptis suaveolens (L) poit (Laminiaceae). Int J Pharm Technol Res. 2009; 1(3): 737-744.

[22] Buganza-Tepole A, Kuhl E. Systems-based approaches toward wound healing. Pediatr Res. 2013; 73(4): 553-563.

[23] Falanga V. Wound healing and its impairment in the diabetic foot. Lancet. 2005; 366(9498): 1736-1743.

[24] Singh MP, Sharma CS. Wound healing activity of Terminalia chebula in experimentally induceddiabetic rats. Int J Pharm Technol Res. 2009; 4(1): 1267-1270.

[25] Najafi F, Tahvilian R, Zangeneh MM, Zangeneh A, Moradi R. Screening of essential oil of Allium sativum for antibacterial effects against Bacillus subtilis. Int J Rec Sci Res. 2016; 7(11): 14172-14176.   

[26] Zangeneh MM, Najafi F, Tahvilian R, Zangeneh A, Moradi R. Assessment of in vitro antibacterial properties of the hydroalcoholic extract of Scrophularia striata Against Staphylococcus aureus (ATCC No. 25923). Int J Pharm Phytochem Res.2017; 9(1): 40-44.      

[27] Tahvilian R, Moradi R, Zhale H, Zangeneh MM, Zangeneh A, Yazdani H, Hajialiani M. Ethnomedicinal plants: in vitro antibacterial effect of essential oil of Pistacia khinjuk. Int J Sci Eng Res.2016; 7(10): 437-447.

[28] Tahvilian R, Moradi R, Hajialiani M, Zangeneh MM, Zangeneh A, Yazdani H, Zhaleh H. Evaluation of antibacterial properties of essential oil of Pistacia khinjuk. Int J Res Pharm Sci. 2017; 7(1): 1-6.

[29] Poorshamohammad C, Souri N, Amini Z, Kosari F, Jamshidpour R, Zangeneh MM, Zangeneh A. Cucurbita moschata: a plant with antibacterial properties. Int J Curr Med Pharm Res.2017; 3(2): 1356-1359.

[30] Shin D, Minn KW. The effect of myofibroblast on contracture of hypertrophic scar. Plast Reconstr Surg. 2004; 113(2): 633-640.

[31] Langton AK, Herrick SE, Headon DJ. An extended epidermal response heals cutaneous wounds in the absence of a hair follicle stem cell contribution. J Invest Dermatol. 2008; 128(5): 1311-1318.

[32] Courtois MC, Sapoval M, Del-Giudice C, Ducloux R, Mirault T, Messas E. Distal revascularization in diabetic patients with chronic limb ischemia. J Mal Vasc. 2015; 40(1): 24-36.

[33] Nayak BS, Pinto-Pereira LM. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. Complement Altern Med. 2006; 21(6): 41-47.

[34] Kumar MS, Sripriya R, Raghavan HV, Sehgal PK. Wound healing potential of Cassia fistula on infected albino rat model. J Surg Res. 2006; 131(2): 283-289.