Evidence-based Phytotherapy & Complementary Medicine Research Center, Alborz University of Medical Sciences, Karaj, Iran. Department of Pharmacognosy, School of Pharmacy, Alborz University of Medical Sciences, Karaj, Iran.
Department of Physiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Student Research Committee, Paramedical School, Alborz University of Medical Sciences, Karaj, Iran.
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.
Research Center for Social Determination of Health, Alborz University of Medical Sciences, Karaj, Iran.
Background and objectives: Berberis vulgaris L (Berberidaceae) is a medicinal plant that is distributed in different parts of Iran; it is grown as a wild or cultivated plant. It has different pharmacological activities such as antioxidant, anti-inflammatory, anti-arrhythmic, sedative and anti-malaria effects. In this study, the anti-seizure activity of different fractions of this plant was evaluated. Methods: Seventy two rats were randomly divided in to nine groups (n=8 in each group). (1): negative control group (normal saline 10mL/kg), (2): positive control group (sodium valproate 1 mg/kg), (3, 4, 5): hydroalcoholic extract-treated groups (100, 200, 400 mg/kg), (6, 7): methanol fraction-treated groups (100 and 200 mg/kg) and (8, 9): chloroform fraction-treated group (100 and 200 mg/kg). Thirty minute after peritoneal injection of different doses of extract, fractions, saline and gavage of sodium valproate, PTZ (45 mg/kg) was injected and they were immediately transferred to a special cage, and the seizure parameters were evaluated for 30 min. Result: The injection of different doses of hydroalcoholic extract and different fractions had a dose-dependent effect on prolongation of latency to the onset of seizures. The effective dose was 400 mg/kg of hydroalcoholic extract and 200 mg/kg of methanol fraction. They decreased the rate of mortality and the number of suddenly seizures jumping significantly. Conclusion: The present study demonstrated that the hydroalcoholic extract and methanol fraction of B. vulgaris showed anticonvulsant activity in PTZ-induced seizures in mice. Therefore, this plant may be more useful in petit mal epilepsy.