Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Background and objectives: The genus Salvia is one of the largest genera in the family Lamiaceae and represents approximately 1000 species displaying a remarkable diversity.Salvia tebesana Bunge(Lamiaceae) is an endemic medicinal species that grows wild in center of Iran which is locally named “Maryamgoli Tabasi”. Methods: The dried roots (2 kg) were perculated with MeOH at room temperature. Methanol extract was further fractionated to give four different fractions [n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc) and water (H2O)]. In the next step, to afford the major bioactive compound(s) of the plant, the EtOAC extract was further sub-fractionated by silica gel column chromatography using a mixture of petroleum ether/ethyl acetate with increasing polarity. The different fractions were purified by reversed-phase semi-preparative HPLC. Results: From the ethyl acetate extract, two diterpene quinones with rearranged abietane skeletons, aegyptinones A and B were isolated and their structures were mainly elucidated by 1D and 2D NMR and mass spectroscopy. Conclusion: These compounds have been reported for the first time from this plant.