Pharmacognostic study of Argyreia pilosa stem

Document Type : Original paper

Authors

1 Department of Pharmacy, JNTUK, Kakinada – 533 003, Andhra Pradesh, India.

2 Department of Pharmaceutical Analysis and Quality Control , Shri Vishnu College of Pharmacy, Bhimavaram, Andhra Pradesh, India.

3 Department of Pharmaceutical Chemistry, University College of Pharmaceutical Sciences, Visakhapatnam, India.

Abstract

Background and objectives: Argyreia pilosa (Convolvulaceae) has been utilized for many aliments in the conventional system ethnomedicinally; most significantly against sexually transmitted diseases, skin troubles, diabetes, rheumatism, cough, and quinsy. The key challenge experienced in the standardization of herbal drugs is the correct identification of the plant source. Thus, setting up quality control parameters by means of pharmacognostic and phytochemical analysis which assures the purity, safety, and efficiency of A. pilosa is necessary. The current research was conducted to assess the pharmacognostic characteristics including macroscopic, microscopic, phytochemical and physicochemical parameters of the stems of A. pilosa. Methods: Micro as well as macroscopic characteristics were investigated. Physicochemical parameters had been done by implementing WHO suggested parameters; preliminary phytochemical and fluorescent evaluation of stem was executed for appropriate identification and standardization.  Results: The color, shape, size, odor and surface characteristics were reported from the stem and powdered stem material of A. pilosa. Light microscope images of cross section and powdered stem revealed the presence of phloem fibers, multicellular uniseriate trichomes, sclerides, lignified xylem fibers, xylem vessels, parenchyma cells and medullary rays. Phytochemical testing confirmed the presence of flavonoids, alkaloids, tannins, phenols, steroids, fixed oils, fats, acid compounds, glycosides, amino acids, and proteins. Physicochemical parameters such as moisture content, ash value, extractive value and fluorescent behavior of stem powder have also been established. Conclusion: The current research would be useful in order to supplement the information regarding to standardization, identity and in performing additional explorations in Ayurvedic system of medicine.
 

Keywords


[1] Houghton PJ, Mukherjee PK. Evaluation of herbal medicinal products: perspectives on quality, safety and efficacy. London: Pharmaceutical press, 2009.
[2] Robinon M, Zhang X. The world medicine situation (traditional medicines: global situation, issues and challenges). Geneva: World Health Organization, 2011.
[3] Vaidya ADB, Devasagayam TPA. Current status of herbal drugs in India: an overview. J Clin Biochem Nutr. 2007; 41(1): 1-11.
[4] Alam F, Najum us Saqib Q. Pharmacognostic standardization and preliminary phytochemical studies of Gaultheria trichophylla. Pharm Biol. 2015; 53(12): 1711-1718.
[5] Nisbet LJ, Moore M. Will natural products remain an important source of drug research for the future? Curr Opin Biotechnol. 1997; 8(6): 708-712.
[6] Gobbo-Neto L, Lopes NP. Plantas medicinais: fatores de influência no conteúdo de metabólitos secundários. Química nova. 2007; 30(2): 374-381.
[7] Bhope SG, Nagore DH, Kuber VV, Gupta PK, Patil MJ. Design and development of a stable polyherbal formulation based on the results of compatibility studies. Pharmacognosy Res. 2011; 3(2): 122-129.
[8] Traiperm P, Staples GW. A new endemic Thai species of Argyreia (Convolvulaceae). Phytotaxa. 2014; 164(4): 281-285.
[9] Chetty KM, Sivaji K, Rao KT. Flowering plants of Chittoor District, Andhra Pradesh, India. Tirupati: Student offset printers, 2008.
[10] Ambasta S. The useful plants of India. New Delhi: CSIR, 1986.
[11] Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomed. 1995; 2(2): 137-189.
[12] Bhandary M, Chandrashekar K, Kaveriappa K. Medical ethnobotany of the siddis of Uttara Kannada district, Karnataka, India. J Ethnopharmacol. 1995; 47(3): 149-158.
[13] Ghorbani A, Langenberger G, Feng L, Sauerborn J. Ethnobotanical study of medicinal plants utilised by Hani ethnicity in Naban river watershed national nature reserve, Yunnan, China. J Ethnopharmacol. 2011; 134(3): 651-667.
[14] Gurib-Fakim A, Sewraj M, Gueho J, Dulloo E. Medicalethnobotany of some weeds of Mauritius and Rodrigues. J Ethnopharmacol. 1993; 39(3): 175-185.
[15] Galani V, Patel B, Patel N. Argyreia speciosa (Linn. f.) sweet: a comprehensive review. Pharmacogn Rev. 2010; 4(8): 172-178.
[16] Lalan BK, Hiray R, Ghongane B. Evaluation of analgesic and anti-inflammatory activity of extract of Holoptelea integrifolia and Argyreia speciosa in animal models. J Clin Diagn Res. 2015; 9(7): 1-4
[17] Yadav KS, Yadav NP, Rawat B, Rai VK, Shanker K, Venkateswara Rao C. An assessment of wound healing potential of Argyreia speciosa leaves.
Sci World J. 2014; Article ID 406921.
[18] Galani VJ, Patel BG. Psychotropic activity of Argyreia speciosa roots in experimental animals. Ayu. 2011; 32(3): 380-384.
[19] Motawi TK, Hamed MA, Hashem RM, Shabana MH, Ahmed YR. Protective and therapeutic effects of Argyreia speciosa against ethanol-induced gastric ulcer in rats. Z Naturforsch C. 2012; 67(1-2) : 47-57.
[20] Singh P. Pharmacognostic study of root and stem of Argyreia speciosa St. Q J Crude Drug Res. 1965; 5(4): 774-783.
[21] Akbar S, Hanif U, Ali J, Ishtiaq S. Pharmacognostic studies of stem, roots and leaves of Malva parviflora L. Asian Pac J Trop Biomed. 2014; 4(5): 410-415.
[22] Kataria S, Rao SK, Bhandari A, Kaur D. Pharmacognostical standardization of Corchorus depressus (L.) Stocks (Tiliaceae)-A promising ethnomedicinal plant. Indian J Tradit Know. 2013; 12(3): 489-497.
[23] Choudhary N, Siddiqui M, Khatoon S. Pharmacognostic evaluation of Tinospora cordifolia (Willd.) Miers and identification of biomarkers. Indian J Tradit Know. 2014; 13(3): 543-550.
[24] World Health Organization. Quality control methods for herbal materials. Geneva: World Health Organization, 2011.
[25] Khandelwal KR. Practical pharmacognosy: techniques and experiments. Maharashtra: Niral Prakashan, 2008.
[26] Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas. Heidelberg: Springer, 1996.
[27] Singh P, Khosa RL, Mishra G, Jha KK. Pharmacognostical evaluation of aerial parts of Graptophyllum pictum (L.) Griff. (Syn: Justicia picta Linn.): a well-known folklore medicinal plant. Anc Sci Life. 2015; 34(4): 223-229.
[28] Rakholiya K, Chanda S. Pharmacognostic, physicochemical and phytochemical investigation of Mangifera indica L. var. kesar leaf. Asian Pac J Trop Biomed. 2012; 2(2): 680-684.
[29] Dave R, Nagani K, Chanda S. Pharmacognostic studies and physicochemical properties of the Polyalthia longifolia var. pendula leaf. Pharmacogn J. 2010; 2(13): 572-576.
[30] Vaghasiya Y, Nair R, Chanda S. Antibacterial and preliminary phytochemical and physico-chemical analysis of Eucalyptus citriodora Hk leaf. Nat Prod Res. 2008; 22(9): 754-762.
[31] Prasanth D, Rao AS, Yejella RP. Assessment of pharmacognostic, phytochemical and physicochemical standards of Aralia racemosa (L.) root. Indian J Pharm Educ Res. 2016; 50(3): 225-230.