Development and validation of a HPLC-UV method for determination of Proscillaridin A in Drimia maritima

Document Type : Original paper

Authors

1 Department of Traditional Pharmacy, Faculty of Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.

2 Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

3 Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.

4 Food and Drug Laboratory Research Center, Food and Drug Control Laboratories, MOH and ME, Tehran, Iran.

Abstract

Background and objectives:Drimia maritima (L.) Stearn also known as squill is a medicinally important plant that has been used for various ailments such as dropsy, respiratory disorders, jaundice and epilepsy from ancient times. Proscillaridin A is identified as one of the most effective compounds in the plant with remarkable pharmacological features including efficacy against congestive heart failure, antitumor, t-cell suppressive and analgesic activities. In the present study, a reliable high performance liquid chromatography (HPLC) method has been developed for quantification of proscillaridin A in D. maritima.  Methods: The separation of compounds was performed using gradient elution (methanol: water) on a reversed phase ACE C18 with flow rate of 1 mL/min and UV detection at 300 nm for 50 min. The method was evaluated using validation parameter such as selectivity, linearity, precision, recovery, limit of detection (LOD) and limit of quantization (LOQ). Results: The separation technique was selective for quantification of proscillaridin A. The calibration graph was linear with r2> 0.998. The intra and inter-day precision (RSD%, 3.8-4.16 and 7.5) were satisfactory. LOD and LOQ were calculated as 0.6 and 1.8 µg/mL respectively. The recovery average was 93.7%.
Conclusion: Due to precision, accuracy and speed, the proposed HPLC-UV method could be applied for determination of proscillaridin A in Drimia maritima samples.

Keywords


[1] Bozorgi M, Amin Gh, Shekarchi M, Rahimi R. Medicinal plants of the genus Drimia: a review on traditional uses, phytochemistry, pharmacology and toxicology. J Tradit Chin Med. 2016. In press.
[2] Royal Botanic Gardens, Kew, Missouri Botanical Garden. The Plant List. Version 1.1; Available from: http://www.theplantlist.org/
[3] Ghahreman A. Flora of Iran. Tehran: Research Institute of Forests and Rangelands, 1997.
[4] Pohl TS, Crouch NR, Mulholland DA. Southern african hyacinthaceae: chemistry, bioactivity and ethnobotany. Curr Org Chem. 2000; 4(12): 1287-1324.
[5] Khare CP. Indian medicinal plants. Berlin: Springer, 2007.
[6] Aliotta G, De-Santo NG, Pollio A, Sepe J, Touwaide A. The diuretic use of scilla from dioscorides to the end of the 18th century. J Nephrol. 2004; 17(2): 342-347.
[7] Avicenna. The canon. Translated by Sharafkandi A. Tehran: Soroush Press, 2008.
[8]  Jorjani SE. Zakhire Kharazmshahi. Tehran: Tehran University of Medical Sciences, 2013.
[9] Gentry HS, Verbiscar AJ, Banigan TF. Red squill. Econ Bot. 1987; 41(2): 267-282.
[10] Stoll A, Suter E, Kreis W, Bussemaker BB, Hofmann A. Die herzaktivenSubstanzen der Meerzwiebel Scillaren A. Helv Chim Acta. 1933; 16(1): 703-733.
[11] Gould L, Fisch S, Cherbakoff A, Degraff AC. Clinical studies on proscillaridin A, a new squill glycoside. J clin pharmacol new drugs. 1971; 11(2): 135-145.
[12] El-Seedi HR, Burman R, Mansour A, Turki Z, Boulos L, Gullbo J, Göransson U. The traditional medical uses and cytotoxic activities of sixty-one Egyptian plants: Discovery of an active cardiac glycoside from Urginea maritima. J Ethnopharmacol. 2013; 145(3): 746-757.
[13] Bielawski K, Winnicka K, Bielawska A. Inhibition of DNA topoisomerases I and II, and growth inhibition of breast cancer MCF-7 cells by ouabain, digoxin and proscillaridin A. Biol Pharm Bull. 2006; 29(7): 1493-1497.
[14] Winnicka K, Bielawski K, Bielawska A, Miltyc W. Apoptosis-mediated cytotoxicity of ouabain, digoxin and proscillaridin A in the estrogen independent MDA-MB-231 breast cancer cells. Arch Pharm Res. 2007; 30(10): 1216-1224.
[15] Winnicka K, Bielawski K, Bielawska A, Miltyc W. Dual effects of ouabain, digoxin and proscillaridin A on the regulation of apoptosis in human fibroblasts. Nat Prod Res. 2010; 24(3): 274-285.
[16] Feng R, Rios JA, Onishi T, Lokshin A, Gorelik E, Lentzsch S. Cell-based and cytokine-directed chemical screen to identify potential anti-multiple myeloma agents. Leukemia Res. 2010; 34(7): 917-924.
[17] Nilubol N, Zhang L, Shen M, Zhang YQ, He M, Austin CP, Kebebew E. Four clinically utilized drugs were identified and validated for treatment of adrenocortical cancer using quantitative high-throughput screening. J Transl Med. 2012; 10: 1-15.
[18] Bayazit V, Konar V. Analgesic effects of scilliroside, proscillaridin-A and taxifolin from squill bulb (Urginea maritima) on pains. Dig J Nanomater Bios. 2010; 5(2): 457-465.
[19] Terness P, Navolan D, Dufter C, Kopp B, Opelz G. The T-cell suppressive effect of bufadienolides: structural requirements for their immunoregulatory activity. Int Immuno Pharmacol. 2001; 1(1): 119-134.
[20] Pascual-Villalobos MJ. Anti-insect activity of bufadienolides from urginea maritima. In: Janick J, Whipkey A. Trends in new crops and new uses. Alexandria: Ashs Press, 2002.
[21] Krenn L, Kopp B. Bufadienolides from animal and plant sources. Phytochemistry. 1998; 48(1): 1-29.
[22] Tittel G, Wagner H. Qualitative und quantitative Analyse von Herzglykosiddrogen durch HPLC-Verfahren. Planta Med. 1980; 39(6): 125-134.
[23] Kopp B, Jurenitsch J, Czernia B, Kubelka W. Separation of cardiac glycosides of Urginea maritima by high-performance liquid chromatography; improvement by serial coupling of several polar reversed phase columns. J Chromatogr. 1983; 257: 137-139.
[24] Verbiscar AJ, Patel J, Banigan TF, Schatz RA. Scilliroside and other scilla compounds in red squill. J Agric Food Chem. 1986; 34(6): 973-979.
[25] Knittel DN, Stintzing FC, Kammerer DR. Simultaneous determination of bufadienolides and phenolic compounds in sea squill (Drimia maritima (L.) Stearn) by HPLC-DAD-MSn as a means to differentiate individual plant parts and developmental stages. Anal Bioanal Chem. 2014; 406(24): 6035-6050.
[26] The European Agency for the Evaluation of Medicinal Products, Human Medicines Evaluation Unit. ICH Q2B: Validation of analytical procedures. London: The European agency for the evaluation of medicinal products. Human medicines evaluation unit, 1996.
[27] Shekarchi M, Hajimehdipoor H, Khanavi M, Adib N, Bozorgi M, Akbariadergani B. A validated method for analysis of Swerchirin in Swertia longifolia by high performance liquid chromatography. Phcog Mag. 2010; 6(21): 13-18.
[28] Hajimehdipoor H, Mokhtarikondori B, Amin Gh, Adib N, Rastegar H, Shekarchi M. Development of a valdated HPLC method for the simultaneous determination of flavonoids in Cuscuta chinensis Lam. By ultra-violet detection. Daru. 2012; 20(1): 57.
[29] Shekarchi M, Ramezany F, Shams Ardekani MR, Lamshöft M, Rastegar H, Eftekhari M,  Khanavi M. An improved HPLC method for determination of colocynthin in colocynth, Res J Pharmacogn. 2015; 2(4): 39-48.