Effect of electromagnetic field on okra (Hibiscus esculentus L.) developmental stages and the effect of okra extract on breast cancer cells

Document Type: Original paper

Authors

1 Department of Plant Biology, Faculty of Biological Science, Kharazmi University, Tehran, Iran.

2 Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Abstract

Background and objectives: Electric, magnetic and electromagnetic fields can act as stress factors with different effects on biological systems. Due to the nutritional and medicinal values, and the increasing electromagnetic radiations, the present study was performed to investigate the effects of the electromagnetic field on the developmental stages and cytotoxic properties of okra.
Methods: Both dry and wet seeds were exposed to electromagnetic field with the intensities of 2 and 4 mT for 60 minutes. MTT assay was applied to evaluate the potential cytotoxic effects of okra extract on MCF-7 cell line. The anatomical structure of leaves in both treated and untreated (control) plants were examined.
Results: The results showed that a field intensity of 4 mT increased the speed of germination of wet treated seeds and the stem length of dry treated seeds. MTT assay revealed no cytotoxicity of the aqueous extracts of okra pods up to the concentration of 100 μg/mL from either the treated or the control plants towards the MCF-7 cell line.
Conclusion: The results suggest that the electromagnetic fields would be able to increase the speed of germination without effects on percentage of germination.

Keywords


[1] Adelakun OE, Oyelade OJ, Ade-Omowaye BI, Adeyemi IA, Van de Venter M. Chemical composition and the antioxidative properties of Nigerian Okra seed (Abelmoschus esculentus Moench) flour. Food Chem Toxicol. 2009; 47(6): 1123-1126.

[2] Rio LC, Rio MM. Effect of electro-magnetic field on the growth characteristics of okra (Abelmoschus Esculentus), tomato (Solanum lycopersicum) and eggplant (Solanum melongena). Int J Sci Res Pub. 2013;35(8): 1-9.

[3] Atawodi SE, Atawodi JC, Idakwo P, Pfundstein B, Haubner R, Wurtele G, Spiegelhalder B, Bartsch H, Owen RW. Evaluation of the polyphenol composition and antioxidant activity of African variety of Dacryodes edulis (G. Don) HJ Lam fruit. J Med Food. 2009; 12(6): 1321-1325.

[4] Ren DD, Chen G. Inhibition effect of okra polysaccharides on proliferation of human cancer cell lines. Food Sci. 2010; 31(21): 353-356.

[5] Monte LG, Santi-Gadelha T, Reis LB, Braganhol E, Prietsch RF, Dellagostin OA, E-Lacerda RR, Gadelha CA, Conceição FR, Pinto LS. Lectin of Abelmoschus esculentus (okra) promotes selective antitumor effects in human breast cancer cells. Biotechnol Lett. 2014; 36(3): 461-469.

[6] Cheng DK. Field and wave electromagnetics. New York: Addison-Wesley, 1989.

[7] Moon JD, Chung HS. Acceleration of germination of tomato seed by applying AC electric and magnetic fields. J Electrostatics. 2000; 48(2): 103-114.

[8] Hamzeloo-Moghadam M, Esmaeili S, Fotoohi F, Naghibi F, Pirani A, Hajimehdipoor H. In vitro evaluation for cytotoxic activity of three Ferula species. Int J Pharm Sci Res. 2013; 4(7): 2673-2676.

[9] Hamzeloo-Moghadam M, Firouzi J, Saeidnia S, Hajimehdipoor H, Jamili Sh, Rustaiyan A, Gohari AR. A cytotoxic hydroperoxy sterol from the brown alga, Nizamuddinia zanardinii. Daru J Pharm Res. 2013; 21: 24.

[10] El-Bakatoushi R. Genetic diversity of winter wheat (Triticum aestivum L.) growing near a high voltage transmission line. Rom J Biol Plant Biol. 2010; 55(2): 71-87.

[11] Bahar M, Majd A, Abdi S. Effects of (ELF) extremely low frequency (50 Hz) AC and DC magnetic fields on lentil germination and seedlings growth. J Theor Appl Phys (Iran Phys J). 2009; 3(2): 12-16.

[12] Belyavskaya NA. Biological effects due to weak magnetic field on plants. Adv Space Res. 2004; 34(7): 1566-1574.

[13] Ilango KB, Pradeep RL, Vetrivel D, Brinda P, Mishra M. Safety evaluation of Abelmoschus esculentus polysaccharide. Int J Pharm Sci Rev Res. 2011; 10(2): 106-110.