Anti-diarrheal and hypoglycemic activities of methanol extract of Calamus rotang L. seed in rat

Document Type: Original paper

Authors

1 Department of Pharmacy, BRAC University, Mohakhali, Dhaka, Bangladesh

2 Department of Pharmacy, BRAC University, Mohakhali, Dhaka, Bangladesh.

Abstract

Background and objectives: Calamus rotang is used in traditional medicine. Regarding the previous phytochemical screening of methanol extract of Calamus rotang L. fruits (MCR), this study was performed to find out the antidiarrheal and hypoglycemic effects of the experimented extract in rodents.
Methods: Castor oil induced diarrheal test was followed for screening anti-diarrheal effect of MCR. In order to explore the hypoglycemic effect, normoglycemic study, oral glucose tolerance test (OGTT) and study on alloxan-induced diabetic rats were carried out. In each experiment, 250 and 500 mg/kg body weight doses of MCR were used.
Results: The plant extract showed pronounced, significant (p < 0.01) antidiarrheal activity on both doses in a dose-dependent manner. At higher dose, MCR showed almost similar antidiarrheal effect as standard loperamide.  In case of normoglycemic test, a single dose of MCR caused a significant (p<0.01) reduction in blood glucose level over time compared to the control group. Although metformin reduced blood glucose more rapidly than MCR at both doses, its extent of reduction in blood glucose level was approximately the same as high dose. In OGTT, MCR was active and comparable to that of the glucose treated control group in both doses.  For alloxan induced diabetic rodents, the study revealed that the MCR extracts could decrease the blood glucose level in both doses over a period of three days.
Conclusion: Considering the results of the present study, further isolation of active components and establishment of the mechanism of action have to be continued in the future.

Keywords


[1] Arokiyaraj S, Radha R, Martin S, Perinbam K. Phytochemical analysis and anti-diabetic activity of Cadaba fruticosa R. Br. Ind  J  Sci Tech.2008; 1(6): 1-4.

[2] Gangadevi V, Yogeswari S, Kamalraj S, Rani G, Muthumary J. The antibacterial activity of Acalypha indica L.  Ind J Sci Tech. 2008; 1(6): 1-5.

[3] Chellaram C, Edward JKP. Anti-inflammatory potential of coral reef associated gastropod, Drupa margariticola. Ind J Sci Tech. 2009; 2(2): 75-77.

[4] Rehan A, Swayam PS, Rakesh M, Rajendran S, Arya KR, Arvind KS. Mild antihyperglycaemic activity in Eclipta alba, Berberis aristata, Betula utilis, Cedrus deodara, Myristica fragrans and Terminalia chebula. Ind J  Sci Tech. 2008; 1(5): 1-6.

[5] Gordon DM. Geographical structure and host specificity in bacteria and the implications for tracing the source of coliform contamination. Microbiology. 2001; 147(pt5): 1079-1085.

[6] Shekhawat N, Vijayvergia R. Investigation of anti-inflammatory, analgesic and antipyretic properties of Madhuca indica GMEL. Int J Mol Med Adv Sci. 2010; 6(2): 26-30.

[7] Rashid M, Hasan CM, Choudhury SAR, Begum B, Rahman S. Ethnopharmacological investigation of medicinal plants of Bangladesh. Bangladesh J Physiol Pharmacol. 1997; 12(1): 25-29.

[8] Basumatary SK, Ahmed  M,  Deka SP. Some medicinal plant leaves used by Boro (tribal) people of Goalpara district, Assam. Nat  Prod Radiance. 2004; 3(2): 88-90.

[9] Kagyung R, Gajurel PR, Pethy P, Singh B. Ethnobotanical studies of Dehang-Debang. biosphere reserve of Arunachal Pradesh with special reference to Memba tribe. Ind J Trad Know. 2010;9(3): 496-501.

[10] Khare CP. Indian medicinal plants: an illustrated dictionary. Heidelberg: Springer, 2004.

[11] Murthy BK, Nammi S, Kota MK, Rao RK, Rao NK, Annapurna A. Evaluation of hypoglycemic and antihyprglycemic effect of Datura metel (Linn.) seeds in normal and alloxan induced diabetic rat. J Ethnopharmacol. 2004; 91(1): 95-98.

[12] Hirschhorn N. The treatment of acute diarrhoea in children. An historical and physiological perspective. Am J Clin Nutr. 1980; 33(3): 637-663.

[13] Lanza RP, Ecker DM, Kuhtreiber WM, Marsh JP, Ringelling J, Chink WL. Transplantation of islets using micro encapsulation: studies in diabetic rodents and dogs. J Mol Med. 2001; 77(1): 206-210.

[14] Rang HP, Dale MM, Ritters JM. The endocrine pancreas and the control of blood glucose. In: Textbook of pharmacology. Simmons B, Beasley S, Eds. London: Longman group Ltd, 1991.

[15] Sumana G, Suryawanshi SA. Effect of Vinca rosea extracts in treatment of alloxan diabetes in male albino rats. Indian J Exp Biol. 2001; 39(8): 748-758.

[16] Sohrab G, Aminpour A. The influence of traditional herbs on fasting blood sugar in diabetes. Iran J Pharm Res. 2004; 3(2): 42.

[17] Yaryura-Tobias JA, Pinto A, Neziroglu F. Anorexia nervosa, diabetes mellitus, brain atrophy and fatty liver. Int J Eat Disord. 2001; 30(3): 350-353.

[18] World Health Organization. Expert Committee on Diabetes Mellitus: Second Report Technical. Report Series 646. Geneva: World Health Organization, 1980.

[19] Trease G, Evans M. Pharmacopoeial and related drugs of biological origin. In: a textbook of pharmacognosy. 15th ed. London: WB Saunders, 2001.

[20] Mitjans M, Garcia L, Marrero E, Vinardell MP. Study of ligmed-A, an antidiarrheal drug based on liguin, on rat small intestine enzyme activity and morphometry. J Vet Pharmacol Ther. 2008; 24(5): 349-351.

[21] Zimmermann M. Ethical guidelines for investigations of experimental pain in conscious animals. Pain. 1983; 16(2): 109-110.

[22] Awouter F, Neimegeers CJE, Lenaert FM, Janssen PAJ.  Delay of castor oil diarrhoea in rats; a new way to evaluate inhibitors of prostaglandin’s biosynthesis. J Pharm Pharmacol. 1978; 30(1): 41-45.

[23] Durschlag M, Wurbel H, Stauffacher M, Von HD. Repeated blood collection in the laboratory mouse by tail incision-modification of an old technique. Physiol Behav. 1996; 60(6): 1565-1568.

[24] Attia AH, Mouneir SM. Evaluation of some medicinal plant extracts for antidiarrhoeal activity. Phytother Res. 2005; 19(6): 481-485.

[25]  Agbor GA, Léopold T, Jeanne NY. The antidiarrhoeal activity of Alchornea cordifolia leaf extract.  Phytother Res. 2004; 18(11): 873-876.

[26] Marles RJ, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1995; 2(2): 137-189.

[27] Price KR, Johnson TI, Fenwick GR. The chemistry and biological significance of saponins in food and feeding stuffs. Crit Rev Food Sci. 1987; 26(1): 22-48.

[28] Di Carlo G, Autore G, Izzo AA, Maibline P, Mascolo N, Viola P, Diurno MV, Capasso F. Inhibition of intestinal motility and secretion by flavonoids in mice and rats: structure activity relationships. J Pharm Pharmacol. 1993; 45(12): 1054-1059.

[29] Rao VSN, Santos FA, Sobreika TT, Souza MF, Melo LL, Silveira ER. Investigation on the gastroprotective and antidiarrheal propertiesof ternatin, a tetramethoxyflavone from Egletes viscosa. Planta Med. 1997; 63(3): 1496-1497.

[30] Maikere-Faniyo R, Van Puyvelde L, Mutwewingabo A, Habiyaremye FX. Study of Rwandese medicinal plants used in the treatment of diarrhea I. J Ethnopharmacol. 1989; 26(2): 101-109.

[31] Sanchez de Medina F, Galvez J, Gonzalez M, Zarzuelo A, Barrett KE. Effects of quercetin on epithelial chloride secretion. Life Sci. 1997; 61(20): 2049-2055.

[32] Su YL, Leung LK, Bi YR, Huang Y, Chen ZY. Antioxidant activity of flavonoids isolated from Scutellaria rehderiana. J Am Chem Soc. 2000; 77(8): 807-812.

[33] Mora A, Paya M, Rios JL, Alcaraz MJ. Structure activity relationships of polymethoxy flavones and other flavonoids as inhibitors of nonenzymic lipid peroxiation. Biochem Pharmacol. 1990; 40(4): 793-797.

[34] Dunn JS, Sheehan HL, Mclechie NG. Necrosis of langerhans produced experimentally. Lancet. 1943; 241(6242): 484-487.

[35] Goldener MG, Gomori G. Studies on the mechanism of alloxan diabetes. Endocrinology. 1964; 35(4): 241-248.

[36] Pari L, Venkateswaran S. Hypoglycaemic activity of Scoparia dulcis L. extract in alloxan induced hyperglycaemic rats. Phytother Res. 2002; 16(7): 662-664.

[37] Twaij HA, Al-Badr AA. Hypoglycemic activity of Artemisia herba-alba. J Ethnopharmacol. 1988; 24(2-3): 123-126.

[38] Kasiviswanath R, Ramesh A, Kumar KE. Hypoglycemic and antihyperglycemic effect of Gmelina asiatica Linn. in normal and in alloxan induced diabetic rats. Biol Pharm Bull. 2005; 28(4): 729-732.

[39] Jayaraman R, Shivakumar A, Anitha T, Vishal DJ, Narahari N, Rom P. Antidiabetic effect of petroleum ether extract of Citrullus colocynthis fruits against streptozotocin-induced hyperglycemic rats. J Biol Plant Biol Bucharest. 2009; 54(2): 127-134.

[40] Marles JR, Farnsworth NR. Antidiabetic plants and their active constituents. Phytomedicine. 1995; 2(2): 123-189.