An Ethnobotanical Study on Medicinal Plants Used as Antidote for Snakebite and as Snake Repellent in the Ejisu-Juabeng District of Ghana

Document Type : Original paper

Authors

Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.

Abstract

Background and objectives: Anecdotal evidence shows that plant remedies used by rural folks to repel snakes and those used during snake envenomation are sometimes effective and offer an appreciable survival rate among victims of snake bites. This study focused on documenting plants that repel snakes from homesteads and those administered as interventions during snake bites among indigenes of Ejisu-Juabeng District, Ghana. Method: Personal interviews with indigenes was carried out. Information about the plants including scientific names, families, local names, growth habit, the used part, method of preparation and administration were recorded. Herbarium vouchers were used to identifying the plants at species level. The frequency of citation (FC) and relative frequency of citation index (RFC) for each species was determined. Results: Twenty-three medicinal plants were documented; 17 plants belonging to 15 genera from 13 families were reported to be used as antidote for snakebite poisoning while ten species belonging to nine genera from nine families were reported as snake repellents. Plants belonging to the family Apocynaceae were the most predominant (12.5%). The anti-venin plants mostly mentioned were herbs (48%) and trees (39%). Leaves (58.8%) and roots (29.4%) were frequently used in antivenin formulations and were mostly applied topically (78.5%) as poultices or orally (21.4%) as infusions/decoctions. For snake repelling plants the strong odour from plants was mostly responsible for the repellent effect. The most commonly named plants were Nicotiana tobacum (Relative frequency of citation=0.26), Allium sativum (RFC=0.14), Rauwolfia serpentine (RFC=0.18) and Allium cepa (RFC=0.18). Conclusion: This study has revealed the importance of herbal medicine used in the prevention and treatment of snakebites among indigenes of Ejisu-Juabeng District.

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  • Chippaux JP. Snakebite envenomation turns again into a neglected tropical disease! J Venom Anim Toxins Incl Trop Dis. 2017; 23(1): 1–2.
  • Gutiérrez JM, Calvete JJ, Habib AG, Harrison RA, Williams DJ, Warrell DA. Snakebite envenoming. Nat Rev Dis Primers. 2017; 3(1): 1–21.
  • Benjamin JM, Abo BN, Brandehoff N. Snake envenomation in Africa. Curr Trop Med Rep. 2020; 7(1): 1–10.
  • Chippaux JP, Massougbodji A, Diouf A, Baldé CM, Boyer LV. Snake bites and antivenom shortage in Africa. 2015; 386(10010): 2252–2253.
  • Chippaux JP, Stock RP, Massougbodji A. Methodology of clinical studies dealing with the treatment of envenomation. 2010; 55(7): 1195–1212.
  • Punguyire D, Baiden F, Nyuzaghl J, Hultgren A, Berko Y, Brenner S, Soghoian S, Adjei G, Niyogi A, Moresky R. Presentation, management, and outcome of snake-bite in two district hospitals in Ghana. Pan Afr Med. 2014; 19(219): 1–9.
  • Abubakar S, Abubakar I, Habib A, Nasidi A, Durfa N, Yusuf P, Larnyang S, Garnvwa J, Sokomba E, Salako L. Pre-clinical and preliminary dose-finding and safety studies to identify candidate antivenoms for treatment of envenoming by saw-scaled or carpet vipers (Echis ocellatus) in Northern Nigeria. 2010; 55(4): 719–723.
  • Visser L, Kyei-Faried S, Belcher D, Geelhoed D, Van Leeuwen JS, Van Roosmalen J. Failure of a new antivenom to treat Echis ocellatus snake bite in rural Ghana: the importance of quality surveillance. Trans R Soc Trop Med Hyg. 2008; 102(5): 445–450.
  • Visser L, Kyei-Faried S, Belcher D. Protocol and monitoring to improve snake bite outcomes in rural Ghana. Trans R Soc Trop Med Hyg. 2004; 98(5): 278–283.
  • Warrell DA. Snake bite. 2010; 375(9708): 77–88.
  • Wannang N, Gyang S, Jimam N, Okwoli S. Snake repellent properties of Canarium schweinfurthii in laboratory animals: a preliminary study. J Pharm Res. 2009; 8(3): 130–133.
  • Ghana Statistical Service. 2010 Population and Housing Census, District Analytical Report- Ejisu Juabeng District. [Accessed 2020]. Available from: www.statsghana.gov.gh.
  • Appiah K, Oppong C, Mardani H, Omari R, Kpabitey S, Amoatey C, Onwona-Agyeman S, Oikawa Y, Katsura K, Fujii Y. Medicinal plants used in the Ejisu-Juaben municipality, Southern Ghana: an ethnobotanical study. 2019; 6(1): 1–27.
  • Ampofo EK, Amponsah IK, Asante-Kwatia E, Armah FA, Atchoglo PK, Mensah AY. Indigenous medicinal plants as biofilm inhibitors for the mitigation of antimicrobial resistance. Adv Pharmacol Pharm Sci. 2020; Article ID 8821905.
  • Steinhorst J, Aglanu LM, Ravensbergen SJ, Dari CD, Abass KM, Mireku SO, Adu Poku JK, Enuameh YA, Blessmann J, Harrison RA. ‘The medicine is not for sale’: practices of traditional healers in snakebite envenoming in Ghana. Plos Negl Trop Dis. 2021; 15(4): 1–23.
  • Faruque MO, Uddin SB, Barlow JW, Hu S, Dong S, Cai Q, Li X, Hu X. Quantitative ethnobotany of medicinal plants used by indigenous communities in the Bandarban District of Bangladesh. Front Pharmacol. 2018; 9(40): 1–12.
  • Nodza GI, Onuminya TO, Ogbu P, Agboola OO, Ogundipe OT. Ethnobotanical survey of medicinal plants used in treating snakebites in Benue, Nigeria. Ann West Univ Timis Ser Biol. 2020; 23(2): 147–158.
  • Omara T, Kagoya S, Openy A, Omute T, Ssebulime S, Kiplagat KM, Bongomin O. Antivenin plants used for treatment of snakebites in Uganda: ethnobotanical reports and pharmacological evidences. Trop Med Health. 2020; 48(6): 1–16.
  • Dharmadasa R, Akalanka G, Muthukumarana P, Wijesekara R. Ethnopharmacological survey on medicinal plants used in snakebite treatments in Western and Sabaragamuwa provinces in Sri Lanka. J Ethnopharmacol. 2016; 179: 110–127.
  • Sarkhel S. Ethnobotanical survey of folklore plants used in treatment of snakebite in Paschim Medinipur District, West Bengal. Asian Pac J Trop Biomed. 2014; 4(5): 416–420.
  • Owuor BO, Kisangau DP. Kenyan medicinal plants used as antivenin: a comparison of plant usage. J Ethnobiol Ethnomed. 2006; 2(1): 1–8.
  • Asase A, Hesse DN, Simmonds MS. Uses of multiple plants prescriptions for treatment of malaria by some communities in Southern Ghana. J Ethnopharmacol. 2012; 144(2): 448–452.
  • Agyare C, Spiegler V, Sarkodie H, Asase A, Liebau E, Hensel A. An ethnopharmacological survey and in vitro confirmation of the ethnopharmacological use of medicinal plants as anthelmintic remedies in the Ashanti region, in the central part of Ghana. J Ethnopharmacol. 2014; 125(Part A): 255–263.
  • Musah Y, Ameade EP, Attuquayefio DK, Holbech LH. Epidemiology, ecology and human perceptions of snakebites in a Savanna community of Northern Ghana. Plos Negl Trop Dis. 2019; 13(8): 1–20.
  • Dey A, Nath De J. Anti-snake venom botanicals used by the ethnic groups of Purulia District, West Bengal, India. J Herbs Spices Med Plants. 2012; 18(2): 152–165.
  • Yirgu A, Chippaux JP. Ethnomedicinal plants used for snakebite treatments in Ethiopia: a comprehensive overview. J Venom Anim Toxins Incl Trop Dis. 2019; 25 (e20190017): 1–15.
  • Asad MHHB, Sabih DE, Yaqab T, Murtaza G, Hussain MS, Hussain MS, Nasir MT, Azhar S, Khan SA, Hussain I. Phospholipases A2: enzymatic assay for snake venom (Naja naja karachiensis) with their neutralization by medicinal plants of Pakistan. Acta Pol Pharm. 2014; 71(4): 625–630.
  • Giovannini P, Howes MJR. Medicinal plants used to treat snakebite in Central America: review and assessment of scientific evidence. J Ethnopharmacol. 2017; 199: 240–256.
  • Ferreira LA, Henriques OB, Andreoni AA, Vital GR, Campos MM, Habermehl GG, de Moraes VL. Antivenom and biological effects of ar-turmerone isolated from Curcuma longa (Zingiberaceae). 1992; 30(10): 1211–1218.
  • Girish K, Mohanakumari HP, Nagaraju S, Vishwanath B, Kemparaju K. Hyaluronidase and protease activities from Indian snake venoms: neutralization by Mimosa pudica root extract. 2004; 75(3): 378–380.
  • Sia F, Vejayan J, Jamuna A, Ambu S. Efficacy of tannins from Mimosa pudica and tannic acid in neutralizing cobra (Naja kaouthia) venom. J Venom Anim Toxins Incl Trop Dis. 2011; 17(1): 42–48.
  • Ushanandini S, Nagaraju S, Nayaka SC, Kumar KH, Kemparaju K, Girish KS. The anti-ophidian properties of Anacardium occidentale bark extract. Immunopharmacol Immunotoxicol. 2009; 31(4): 607–615.
  • Adeyi AO, Ajisebiola BS, Adeyi OE, Adekunle O, Akande OB, James AS, Ajayi BO, Yusuf PO, Idowu BA. Moringa oleifera leaf fractions attenuated Naje haje venom-induced cellular dysfunctions via modulation of NRF-2 and inflammatory signaling pathways in rats. Biochem Biophys Rep. 2021; 25(100890): 1–11.
  • Ajisebiola BS, Rotimi S, Anwar U, Adeyi AO. Neutralization of Bitis arietans venom-induced pathophysiological disorder, biological activities and genetic alterations by Moringa oleifera leaves. Toxin Rev. 2020; 1(2020): 1–12.
  • Sivaraman T, Sreedevi NS, Meenachisundharam S, Vadivelan R. Neutralizing potential of Rauvolfia serpentina root extract against Naja naja venom. Braz J Pharm Sci. 2020; 56(18050): 1–10.
  • Renapurkar D, Tare T, Sutar N, Deshmukh P. Observations on snake repellent property of some plant extracts. Def Sci J. 1991; 41(1): 79–
  • Ilondu E, Lemy E. Studies on the diversity of snake repellent plants within some communities in Delta state, Nigeria. Int J Plant Ani Environ Sci. 2018; 8(1): 16–24.
  • Oladeji OS, Adelowo FE, Ayodele DT, Odelade KA. Phytochemistry and pharmacological activities of Cymbopogon citratus: a review. Sci Afr. 2019; 6(e00137): 1–11.
  • Clark L, Shivik J. Aerosolized essential oils and individual natural product compounds as brown treesnake repellents. Pest Manag Sci. 2002; 58(8): 775–783.
  • Khair-ul-Bariyah S, Ahmed D, Ikram M. Ocimum basilicum: a review on phytochemical and pharmacological studies. Pak J Chem. 2012; 2(2): 78–85.
  • Benelli G, Pavela R, Maggi F, Wandjou JGN, Koné-Bamba D, Sagratini G, Vittori S, Caprioli G. Insecticidal activity of the essential oil and polar extracts from Ocimum gratissimum grown in Ivory Coast: efficacy on insect pests and vectors and impact on non-target species. Ind Crop Prod. 2019; 132: 377–385.
  • Soares AM, Ticli FK, Marcussi S, Lourenço MV, Januario AH, Sampaio SV, Giglio JR, Lomonte B, Pereira PS. Medicinal plants with inhibitory properties against snake venoms. Curr Med Chem. 2005; 12(22): 2625–2641.  
  • Sajon SR, Sana S, Rana S. Anti-venoms for snake bite: a synthetic and traditional drugs review. J Pharmacogn Phytochem. 2017; 6(3): 190–197.
  • Samy RP, Thwin MM, Gopalakrishnakone P, Ignacimuthu S. Ethnobotanical survey of folk plants for the treatment of snakebites in Southern part of Tamilnadu, India. J Ethnopharmacol. 2008; 115(2): 302–312.