Pharmacognostical and physico-chemical evaluations of Cardiospermum halicacabum L. seeds

Document Type : Original paper

Authors

1 Department of Pharmaceutical Sciences, Saurashtra University, Rajkot, Gujarat, India.

2 B. K. Mody Government Pharmacy College, Rajkot, Gujarat, India.

3 Institute of Ayurvedic Medicinal Plant Sciences, Gujarat Ayurveda University, Jamnagar, Gujarat, India.

Abstract

Background and objectives: Cardiospermum halicacabum L. is a climber plant of Sapindaceae family with certain medicinal properties that have made it to be used traditionally in different systems of medicine such as Ayurveda, Homeopathy and Unani medicine. Literature survey has revealed the reports about some investigations but references were not available about the seeds of C. halicacabum; hence, the seeds were selected for the present investigation. Methods: Detailed macroscopic and microscopic studies of C. halicacabum seeds and powder were carried out, physico-chemical parameters, preliminary and quantitative phytochemical analyses of different extracts of the seeds performed. Besides, the amount of total phenols and flavonoids in different extracts were determined. Results: The phytochemical screening gave a positive test for presence of various phytoconstituents like alkaloids, flavanoids, and tannins. The amount of total phenols was found to be between 29.697±0.232 to 187.372±0.615 mg pyrogallol equivalent/g and the content of flavonoids between 27.833±0.412 to 139.261±0.412 mg quercetin equivalent/g in various extracts.   Conclusion: The present work has reported the detailed macroscopic, microscopic and physio-chemical evaluations of the seeds of C. halicacabum and provided information which would be helpful for better identification and standardization of C. halicacabum seeds.

Keywords


[1] Department of AYUSH. The Ayurvedic Pharmacopoeia of India. New Delhi: Ministry of health & family welfare, government of India, 2007.
[2] Department of AYUSH. The Unani Pharmacopoeia of India. New Delhi: Ministry of health & family welfare, government of India, 2008.
[3] Kirtikar KR, Basu BD. Indian medicinal plants. 2nd ed. Dehradun: International book distributors, 2005.
[4] Venkatesh-Babu KC, Krishnakumari S. Cardiospermum halicacabum suppresses the production of TNF-alpha and nitric oxide by human peripheral blood mononuclear cells. Afr J Biomed Res. 2006; 9(2): 95-99. 
[5] Neuwinger HD. African traditional medicine: a dictionary of plant use and applications. Stuttgart: Medpharm GmbH Scientific Publishers, 2000. 
[6] Ahmad J, Malik AA. Botanicals used in unani system of medicine for cancer chemoprevention and cancer therapy. In: Arora R. Ed. Herbal medicine - a cancer chemopreventive and therapeutic perspective, New Delhi: Jaypee Brothers Medical Publishers, 2010.
[7] Subramanyam R, Newmaster SG, Paliyath G, Newmaster CB. Exploring ethnobiological classifications for novel alternative medicine: a case study of Cardiospermum halicacabum L. (modakathon, balloon vine) as a traditional herb for treating rheumatoid arthritis. Ethnobotany. 2007; 19(1): 1-16. 
[8] Theodore C. The flora of the presidency of the Bombay. 2nd ed. Calcutta:  Botanical survey of India, 1967.
[9] National institute of science communication and information reassures. The Wealth of India. New Delhi: CSIR, 2006. 
[10] Hookert JD. The flora of British India. London: L. Reeve, 1875.
[11] Gopta AK, Sharma M. Indian medicinal plants. New Delhi : ICMR, 2007.
[12] World Health Organization. Quality control methods for herbal materials. Geneva: WHO Press, 2011.
[13] Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. 42nd ed. Pune: Nirali Prakashan, 2008.
[14] Harborne JB. Phytochemical methods a guide to modern techniques of plant analysis. Berlin: Springer Verlag, 2005.
[15] Khandelwal KR. Practical pharmacognosy, techniques and experiments. 17th ed. Pune: Nirali Prakashan, 2007.
[16] Olajire AA, Azeez L. Total antioxidant activity, phenolic, flavonoid and ascorbic acid contents of Nigerian vegetables. Afr J Food Sci. 2011; 2(2): 022-029.
[17] Kim DO, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem. 2003; 81(3):321-326.
[18] Zalke AS, Duraiswamy B, Gandagule UB, Singh N. Pharmacognostical evaluation of Cardiospermum halicacabum Linn. leaf and stem. Ancient Sci Life. 2013; 33(1):15-21.
[19] Ferrara I, Schettino O, Motesano D. Triterpenoids from Cardiospermum halicacabum Linn. Phytother Res. 1996; 10(1): 192-194.
[20] Srinivas K, Choudhary KA, Rao SS, Sathyanarayana T, Rao RV. Phytochemical investigation of Cardiospermum halicacabum Linn. Indian J Nat Prod. 1998; 14(1): 24-27.
[21] Huma S, Ghazala Hr, Shaukat M, Raheela K, Hina Z. In Vitro antimicrobial and phytochemical analysis of Cardiospermum halicacabum L. Pak J Bot. 2012; 44(5): 1677-1680.
[22] Annadurai A, Elangovan V, Velmurugan S, Ravikumar R. Preliminary phytochemical screening and antibacterial of Cardiospermum halicacabum L. Adv Appl Sci Res. 2013; 4(5): 302-308.
[23] Mohaddesi B, Dudhrejiya A, Sheth N. Anticancer screening of various seed extract of Cardiospermum halicacabum on human colorectal, skin and breast cancer cell lines. Arch Breast Cancer. 2015; 2(3): 91-95.
[24] Burr GO, Burr MM, Miller E. On the nature and role of the fatty acids essential in nutrition. J Biol Chem. 2007; 86 (587): 1-17.