Optimization of Ultrasound-assisted Acidic-solvent Extraction of Colchicine from Colchicum kurdicum (Bornm.) Stef. Using Response Surface Methodology

Document Type: Original paper

Authors

1 Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

2 Pharmaceutical Cares Department, Ayatollah Khamenei Hospital, Mazandaran University of Medical Sciences, Abbas Abad, Iran.

3 Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

4 Department of Plant Systematics, High Educational of Sanna Institute, Sari, Iran.

5 Department of Medicinal Chemistry and Pharmaceutical Sciences Research Centre, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

6 Active Pharmaceutical Research Centre (APIRC), Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.

Abstract

Background and objectives: Colchicum kurdicum (Bornm.) Stef. is a flowering perennial monocotyledon plant that has many important bioactive compounds especially colchicine and colchicine derivatives. In this study, the ultrasound-assisted acidic-solvent extraction method coupled with response-surface method was presented as the successful method for large scale extraction of colchicine as an alkaloid compound from Colchicum kurdicum. Methods: According to the literatures, methanol/deionized water (70:30) solvent system was selected for the extraction. In addition, the response-surface method was used for analysis and optimization of colchicine extraction by ultrasonic-assisted acidic-solvent extraction method. Subsequently, colchicine was extracted using this method and the effects of solvent pH, extraction time, solvent/plant ratio, power, and temperature were evaluated. Results: After all analysis procedures, 0.99 mg colchicine/g dried corms was achieved with the following conditions: solvent pH 4, Extraction time 120 minutes, solvent/plant ratio 20 mL/g, power 100 W, and temperature 60 ºC. Conclusion: According to this study, ultrasonic-assisted acidic-solvent extraction was found an effective method for extraction of colchicine from Colchicum kurdicum (Bornm.) Stef. compared to other extraction methods.

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