Document Type: Original paper
Department of Pharmacognosy and Biotechnology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Pharmaceutical Cares Department, Ayatollah Khamenei Hospital, Mazandaran University of Medical Sciences, Abbas Abad, Iran.
Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Plant Systematics, High Educational of Sanna Institute, Sari, Iran.
Department of Medicinal Chemistry and Pharmaceutical Sciences Research Centre, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.
Active Pharmaceutical Research Centre (APIRC), Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran.
Background and objectives: Colchicum kurdicum (Bornm.) Stef. is a flowering perennial monocotyledon plant that has many important bioactive compounds especially colchicine and colchicine derivatives. In this study, the ultrasound-assisted acidic-solvent extraction method coupled with response-surface method was presented as the successful method for large scale extraction of colchicine as an alkaloid compound from Colchicum kurdicum. Methods: According to the literatures, methanol/deionized water (70:30) solvent system was selected for the extraction. In addition, the response-surface method was used for analysis and optimization of colchicine extraction by ultrasonic-assisted acidic-solvent extraction method. Subsequently, colchicine was extracted using this method and the effects of solvent pH, extraction time, solvent/plant ratio, power, and temperature were evaluated. Results: After all analysis procedures, 0.99 mg colchicine/g dried corms was achieved with the following conditions: solvent pH 4, Extraction time 120 minutes, solvent/plant ratio 20 mL/g, power 100 W, and temperature 60 ºC. Conclusion: According to this study, ultrasonic-assisted acidic-solvent extraction was found an effective method for extraction of colchicine from Colchicum kurdicum (Bornm.) Stef. compared to other extraction methods.