TY - JOUR ID - 130034 TI - Holothurin B Isolated from Holothuria atra Inhibits Angiogenesis More Potent than Curcumin in Vitro JO - Research Journal of Pharmacognosy JA - RJP LA - en SN - 2345-4458 AU - Yegdaneh, Afsaneh AU - Safaeian*, Leila AU - Mirian, Mina AU - Dana, Nasim AU - Taheri, Mohadeseh AD - Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. AD - Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. AD - Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. AD - Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran. Y1 - 2021 PY - 2021 VL - 8 IS - 3 SP - 33 EP - 40 KW - angiogenesis inhibitors KW - cell migration inhibition KW - Cell proliferation KW - holothurin B KW - human umbilical vein endothelial cells DO - 10.22127/rjp.2021.277328.1687 N2 - Background and objectives: Triterpene glycosides as the most bioactive components of sea cucumbers, have been considered for their various pharmacological properties especially anticancer and anti-metastasis activities. Due to the limited information on the biological properties of holothurin B as a marine triterpene glycoside, the present study aimed to examine its effect on angiogenesis and compare it with curcumin usinghuman umbilical vein endothelial cells (HUVECs). Methods: Holothurin B was isolated from Holothuria atra and identified by NMR and Mass spectroscopic data. Cell survival was estimated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique and migration of cells was assessed by Transwell test. Angiogenesis was evaluatedin vitro by tube formation assay. Results: Holothurin B reduced HUVECssurvival with IC50 value of 8.16 µg/mL. At the concentrations of 5 and 7.5 µg/mL, it significantly decreased the number of migrated cells, the average length and size of tubules, and mean number of junctions; it was more potent than curcumin. Conclusion: Holothurin B could be considered as a potent antiangiogenic constituent through suppressing endothelial cell proliferation, migration and tubulogenesis in vitro, suggesting its potential for further animal and clinical investigations.  UR - https://www.rjpharmacognosy.ir/article_130034.html L1 - https://www.rjpharmacognosy.ir/article_130034_295f1fe86421bcce0f9aab21a2117316.pdf ER -