- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
Anti-Anxiety Effect of Salvia hypoleuca
1
4
EN
Hamid Reza
Monsef Esfhahani
0000-0001-8999-2250
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
monsefes@tums.ac.ir
Keyvan
Amirshahrokhi
Department of Pharmacology and Toxicology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.
amirshahrokhi@gmail.com
Hoda
Babaei Boroujeni
Department of Pharmacognosy, Faculty of Pharmacy, International Campus, Tehran University of Medical Sciences, Tehran, Iran
babaeihoda@yahoo.com
Ahmadreza
Dehpour
Department of Pharmacology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
dehpoura@sina.tums.ac.ir
Mansour
Miran*
0000-0002-3156-9123
Department of Pharmacognosy and Biotechnology, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran.
10.22127/rjp.2020.216084.1550
<strong> <span class="s16"><span class="bumpedFont20">Background and objectives:</span></span></strong><span class="s4"><span class="bumpedFont20"><strong> </strong>Anxiety is a chronic and common disorder worldwide and impairs the quality of life of affected people. Herbal medicines have long been used to treat CNS related disorders. </span></span><span class="s17"><span class="bumpedFont20">Salvia</span></span><span class="s4"><span class="bumpedFont20"> species are important medicinal plants that have shown various pharmacologic activities including CNS effects. </span></span><span class="s17"><span class="bumpedFont20">Salvia </span></span><span class="s17"><span class="bumpedFont20">hypoleuca</span></span><span class="s4"><span class="bumpedFont20"> is an annual and herbaceous plant which is endemic to Iran. The aim of this study was to evaluate the anxiolytic activity of the hydro-alcoholic, n-hexane, ethyl acetate and methanol extract of </span></span><span class="s17"><span class="bumpedFont20">S. </span></span><span class="s17"><span class="bumpedFont20">hypoleuca</span></span><span class="s4"><span class="bumpedFont20"> in mice. </span></span><strong><span class="s16"><span class="bumpedFont20">Methods:</span></span><span class="s4"><span class="bumpedFont20"> </span></span></strong><span class="s17"><span class="bumpedFont20">n</span></span><span class="s4"><span class="bumpedFont20">-hexane, ethyl acetate, methanol and </span></span><span class="s4"><span class="bumpedFont20">hydroalcholic</span></span><span class="s4"><span class="bumpedFont20"> extracts were prepared from aerial parts of </span></span><span class="s17"><span class="bumpedFont20">Salvia</span></span><span class="s4"><span class="bumpedFont20"> </span></span><span class="s17"><span class="bumpedFont20">hypoleuca</span></span><span class="s4"><span class="bumpedFont20">. Anxiolytic activity of the extracts was evaluated using open field and hole-board tests. The mice were randomly divided into different groups and were treated with normal saline, diazepam, and the extracts of </span></span><span class="s17"><span class="bumpedFont20">S</span></span><span class="s4"><span class="bumpedFont20">. </span></span><span class="s17"><span class="bumpedFont20">hypoleuca</span></span><span class="s4"><span class="bumpedFont20"> (500 mg/kg, </span></span><span class="s4"><span class="bumpedFont20">ip</span></span><span class="s4"><span class="bumpedFont20">). </span></span><strong><span class="s16"><span class="bumpedFont20">Results: </span></span></strong><span class="s4"><span class="bumpedFont20">The results of open field and hole-board tests showed that treatment of mice with the hydro-alcoholic and methanol extracts of </span></span><span class="s17"><span class="bumpedFont20">S</span></span><span class="s4"><span class="bumpedFont20">. </span></span><span class="s17"><span class="bumpedFont20">hypoleuca</span></span><span class="s4"><span class="bumpedFont20">(500 mg/kg, </span></span><span class="s4"><span class="bumpedFont20">ip</span></span><span class="s4"><span class="bumpedFont20">) produced a significant anxiolytic effect as compared to the normal control group. </span></span><span class="s16"><span class="bumpedFont20"><strong>Conclusion:</strong> </span></span><span class="s4"><span class="bumpedFont20">This study demonstrated that the hydro-alcoholic and methanol extracts of aerial parts of </span></span><span class="s17"><span class="bumpedFont20">S. </span></span><span class="s17"><span class="bumpedFont20">hypoleuca</span></span><span class="s4"><span class="bumpedFont20"> have anti-anxiety activity. The isolation and identification of the active compounds of the effective extracts are suggested.</span></span>
anti-anxiety,behavior tests,Salvia hypoleuca
https://www.rjpharmacognosy.ir/article_104694.html
https://www.rjpharmacognosy.ir/article_104694_1e9c24790738b59742fc35c566687549.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
A Validated HPLC Method for Quantitation of Rosmarinic Acid in a Polyherbal Syrup
5
11
EN
Sara
Zakerin
0000-0003-0265-7631
Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Homa
Hajimehdipoor
0000-0002-2739-3399
Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
hmehdipoor@tums.ac.ir
Seyed Alireza
Mortazavi*
0000-0001-5733-7921
Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Rasool
Choopani
Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Shirin
Fahimi
0000-0003-2443-7621
Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
s.fahimi@sbmu.ac.ir
Masoumeh
Sabetkasaei
Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Fatemeh
Tavakolifar
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
10.22127/rjp.2019.205478.1527
Herbal medicines play a significant role in global healthcare systems. In this investigation, “Monzej soda” syrup was prepared by decocting a mixture containing Lavandula angustifolia, Melissa officinalis, Echium amoenum, Cordia myxa, Glycyrrhiza glabra, Ziziphus jujuba, Foeniculum vulgare, Fumaria parviflora, Adiantum capillus-veneris and Alhagi spp. Manna. along with glycerin, sodium benzoate and potacium sorbate. Physicochemical characteristics of the syrup were examined. Moreover, an HPLC technique was designed for analysis of rosmarinic acid in the syrup using C18 column, isocratic H3PO4 0.085% as the mobile phase, flow rate of 1 ml/min in λmax 330 nm. The method was validated for selectivity, linearity, precision, accuracy, LOD and LOQ. The herbal syrup was brown color with special taste and flavor. Density, pH, viscosity, dry residue, total phenolics, rosmarinic acid content were found 1.085 g/ml, 5.56, 5.35 cP, 15.22±0.43 %, 194 mg/100ml and 47.5 mg/100 ml, respectively. The syrup was stable during laboratory stability tests. The HPLC method was valid according to selectivity, linearity (72-110 µg/ml, r2: 0.9995), intra-day and inter-day precisions (RSD%˂2), accuracy (103.38-106.47%), LOD 1.6 µg/ml and LOQ 4.9 µg/ml. The syrup is a good candidate for pharmaceutical companies after pharmacological and clinical tests.
depression,HPLC,Iranian traditional medicine,rosmarinic acid,Validation
https://www.rjpharmacognosy.ir/article_100220.html
https://www.rjpharmacognosy.ir/article_100220_e12fc2538c2a6d7d14bb889016d4624d.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
The Anxiolytic and Antidepressant Effect of Tanacetum polycephalum in the Pentylenetetrazole Kindled Rats
13
20
EN
Vahid
Azizi
0000-0001-6131-0318
Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
v_azizi@sbu.ac.ir
Farzin
Allahyari
Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran
f.allahyari@mail.sbu.ac.ir
Farnoosh
Rezaali
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
rezaali.farnoosh@gmail.com
Abdolkarim
Hosseini*
0000-0002-1424-7843
Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.
ab_hosseini@sbu.ac.ir
10.22127/rjp.2020.211810.1541
Background and objective: Epilepsy one the most prevalent neurological illness which affects behavioral statuses like anxiety, depression and balance. Based on the anti-inflammatory and antioxidant properties of Tanacetum polycephalum, its effect on anxiety and depression caused by pentylenetetrazole (PTZ)-kindling in rats was investigated. Methods : In order to prepare the required extract, aerial part of the Tanacetum was powdered (100 g) and macerated in 1 L of ethanol (80%) for 24 h. Twenty-four rats were randomly assigned to four groups: PTZ (sub-threshold dose 35 mg/kg for one month, intraperitoneal (i.p)), PTZ + phenobarbital (30 mg/kg, i.p), PTZ + Tanacetum extract (300 mg/kg, i.p), and PTZ + Tanacetum extract (600 mg/kg, i.p). Elevated plus maze (EPM), forced swim test (FST) and rotarod test were employed to assess the anxiety parameters, antidepressant and balance potential, respectively. Results: Tanacetum extract administered at the doses of 300, and 600 mg/kg, exert antidepressant-like activity in the FST test and reduced the immobility time. In the EPM test, Tanacetum extract at the same doses produced anxiolytic-like effects. Also, the rats which received Tanacetum extract showed a significant improvement in the rotarod test in contrast to the PTZ group. Conclusion: The finding from current study showed that Tanacetum could ameliorate neurobehavioral parameters of anxiety and depression in the PTZ-kindled rats.
Anxiety,depression,Epilepsy,Pentylenetetrazole,Tanacetum
https://www.rjpharmacognosy.ir/article_103900.html
https://www.rjpharmacognosy.ir/article_103900_155e87a0ec07105632447335116c2061.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
Chemical Composition and Biological Activity of Ferula aucheri Essential Oil
21
31
EN
Sheyda
Ahmadi koulaei
0000-0003-2145-0935
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
sheydaahmadi@ymail.com
Abbas
Hadjiakhoondi
0000-0001-9172-3543
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
abbhadji@tums.ac.ir
Mohammad Reza
Delnavazi
https://orcid.org/00
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
delnavazi@tums.ac.ir
Zahra
Tofighi
0000-0001-8909-6595
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
ztofighi@tums.ac.ir
Yousef
Ajani
http://orcid.org/000
Research Institute of Forest and Rangelands, Tehran, Iran.
ajanisef@yahoo.com
Fatemeh
Kiashi
0000-0002-5363-3560
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
f.kiashy@gmail.com
Narguess
Yasa*
0000-0002-2455-0053
Department of Pharmacognosy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
yasa@sina.tums.ac.ir
10.22127/rjp.2020.210354.1537
<strong>Background and objectives: </strong>Antibiotics resistance and unpleasant side effects of AChE inhibitors have led to an increased interest in herbs as potential sources. <em>Ferula aucheri</em> (Syn: <em>Dorema aucheri</em>) an indigenous species of <em>Ferula</em> (Apiaceae) grows in Iran and is used as food and medicinal plant. The present study was aimed to identify the oil composition and evaluate antimicrobial and AChE inhibitory activity of flowering tops, fruits and roots. <strong>Methods:</strong> The chemical composition of the oils was recognized by GC and GC‐MS. The antimicrobial effects were assessed on 12 microorganisms by disc diffusion and micro-well dilution methods and AChE inhibitory potentials by a modified version of Ellman's method. <strong>Results:</strong> Sixty five compounds were identified from different organs and the notable characteristics have been high amounts of sesquiterpenes. Germacrene B (14.96%) and β-caryophyllene (12.87%) were distinguished as major components of flowering tops. Cis-dihydroagarofuran (9.02%) and δ-cadinene (8.28%) were identified as the remarkable constituents of fruit. δ-cadinene (18.25%) and gurjunene (12.62%) were detected from the roots by high content. All volatile oils exhibited lower MICs on <em>Bacillus subtilis</em>, <em>Klebsiella pneumonia</em>, <em>Shigella dysenteriae</em>,and <em>Salmonella paratyphi</em>-A serotype compared with gentamicin. Root and fruit oils were more effective than gentamicin against <em>Escherichia coli</em> and flowering tops oils proved lower MICs versus <em>Staphylococcus aureus</em>. Fruits and root oils showed weak potency for inhibiting AChE with IC<sub>50 </sub>values 554.05±4.65 and 239.69±3.5 μg/mL, respectively and flowering tops exhibited moderate activity (179.06±4.3 μg/mL). <strong>Conclusion:</strong> The findings demonstrated that <em>F. aucheri</em> essential oils possessed antimicrobial activities with inhibition properties toward AchE.
AChE inhibitory activity,Antimicrobial activity,Dorema aucheri,Essential oil,Ferula aucheri
https://www.rjpharmacognosy.ir/article_102389.html
https://www.rjpharmacognosy.ir/article_102389_528f7bc824f07f755f64a2e09391cfdd.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
Assessment of Saffron Neuroprotective Properties in Rat Retina versus Light Damage
33
42
EN
Nayebali
Ahmadi
0000-0003-2243-8276
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mohammadreza
Razzaghi
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Maryam
Hamzeloo-Moghadam
0000-0001-7183-9856
Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
mhmoghadam@sbmu.ac.ir
Mohammad
Rostami-Nejad
0000-0003-2495-1831
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
m.rostamii@gmail.com
Majid
Rezaei-Tavirani
0000-0001-6031-7278
Firoozabadi Hospital, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Mohammad Hossein
Heidari
0000-0002-1391-2258
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Saeed
Safari
Proteomics Research Center, Department of Emergency Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mostafa
Rezaei-Tavirani*
0000-0003-1767-7475
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
tavirany@yahoo.com
10.22127/rjp.2020.219620.1555
<span class="s18"><span class="bumpedFont20"><strong>Background and objectives:</strong> </span></span><span class="s19"><span class="bumpedFont20">Crocus sativus</span></span><span class="s4"><span class="bumpedFont20"> L. (Iridaceae) commonly known as saffron, is a popular spice which is used for its pleasant aroma and favored color. Regarding the previous reports about the neuroprotective behavior of saffron or its constituents, in the present work, the neuroprotective property of saffron in rat retina was investigated against light damage in a system biology study. </span></span><strong><span class="s18"><span class="bumpedFont20">Methods:</span></span></strong><span class="s4"><span class="bumpedFont20"><strong> </strong>Retina gene profiles of 4 groups (each group including 3 samples) </span></span><span class="s4"><span class="bumpedFont20">o</span></span><span class="s4"><span class="bumpedFont20">f rats (control; C light damage; L, Saffron; S, and saffron-light damage; SL) which are included in GSE22818 were extracted from Gene Expression Omnibus (GEO). The significant differentially expressed genes (DEGs) from C-L groups analysis which are not included in S-SL comparison were screened by pathway analysis to find the critical protected genes against light damage by saffron. </span></span><span class="s18"><span class="bumpedFont20"><strong>Results:</strong> </span></span><span class="s4"><span class="bumpedFont20">Numbers of 46 gene were protected by saffron versus light damage significantly. The findings revealed that Casp3, Myd88, Birc3, Tnfrsf1a, </span></span><span class="s4"><span class="bumpedFont20">Myc</span></span><span class="s4"><span class="bumpedFont20">, Nfkb2, Fgf2 were the important protected genes by saffron against light damage. “MAPK signaling pathway” and “apoptosis” were highlighted as important related pathways for 46 DEGs. </span></span><span class="s18"><span class="bumpedFont20"><strong>Conclusion:</strong> </span></span><span class="s4"><span class="bumpedFont20">Saffron protects a part of light damage which is controlled mostly by Casp3, Myd88, Birc3, Tnfrsf1a, </span></span><span class="s4"><span class="bumpedFont20">Myc</span></span><span class="s4"><span class="bumpedFont20">, Nfkb2, Fgf2. It seems other parts of damage should be studied in more details to find a complete prospective of molecular mechanism of light damage effect on retina. </span></span>
Crocus sativus,genetic association studies,Rats,Retina,Saffron
https://www.rjpharmacognosy.ir/article_104134.html
https://www.rjpharmacognosy.ir/article_104134_c3392a3843bb1a3cd97c45809a11a5b3.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
The Role of Allium saralicum Extract on Prevention of Acetaminophen-Induced Hepatic Failure: an Experimental Study
43
51
EN
Mahdi
Alvandi
0000-0001-6779-6099
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
dr.mahdi_alvandi@yahoo.com
Dara
Dastan
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.Department of Pharmacognosy, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
d.dastan@umsha.ac.ir
Sara
Soleimani Asl
0000-0003-1518-3308
Anatomy Department, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
sr_soleimani@yahoo.com
Amir
Nili-Ahmadabadi*
0000-0002-7838-9090
Medicinal Plants and Natural Products Research Center, Hamadan University of Medical Sciences, Hamadan, Iran. Department of Pharmacology and Toxicology, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan, Iran.
10.22127/rjp.2020.207706.1533
<strong>Background and objectives:</strong> Acetaminophen (APAP) is a common analgesic medicine whose overdose leads to severe hepatic dysfunction. Due to the known antioxidant properties of <em>Allium</em>species, the present study aimed to evaluate the protective effects of <em>Allium</em><em> saralicum </em>plant on APAP induced liver toxicity.<strong> Methods:</strong> The hydro-alcoholic extract of <em>A.</em><em> saralicum </em>was prepared by maceration and ultrasonic methods. Forty-two rats in seven groups were treated by gavage as follows: groups 1 and 2 received normal saline, groups 3 received 400 mg/kg of <em>A.</em><em> saralicum </em>hydro-alcoholic extract, and the groups 4-7 were treated with 50, 100, 200 and 400 mg/kg of <em>A.</em><em> saralicum </em>extract, respectively. After two consecutive weeks, the therapeutic groups, as well as the positive control (APAP) group, were administered a single dose of APAP (2 g/kg). After 48 hours, the animals were anesthetized, and blood and liver samples were collected for histological and biochemical examinations.<strong>Results:</strong> Our findings indicated that APAP caused a significant rise in ALT (p<0.001), AST (p<0.001), ALP (p<0.001) and LDH (p<0.001) serum levels, total and direct bilirubin (p<0.001), hepatic lipid peroxidation (LPO; p<0.001) and nitric oxide (NO; p<0.001). In addition, APAP let to the decreasing of the total antioxidant capacity (TAC; p< 0.001), total thiol molecules (TTM; p<0.001), and structural changes in the hepatic tissue. Following administration of<em>A.</em><em> saralicum </em>extract, a remarkable improvement was observed in the functional and oxidative stress indices of liver tissue alongside histopathologic alterations.<strong> Conclusion:</strong> Our results showed that <em>A.</em><em> saralicum </em>extractsignificantly improved APAP-induced hepatic failure through inhibition of oxidative/nitrosative stress.
Acetaminophen,Allium saralicum,hepatotoxicity,oxidative stress
https://www.rjpharmacognosy.ir/article_103488.html
https://www.rjpharmacognosy.ir/article_103488_73d8f02840824681563cba0a53c56099.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
In Vitro Anti-adenovirus Activity and Antioxidant Potential of Pistacia atlantica Desf. Leaves
53
60
EN
Ali
Karimi
0000-0003-1554-3668
Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
kakarimi42@skums.ac.ir
Mohammad-Taghi
Moradi*
0000-0002-3103-3740
Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.
mtmoradi65@gmail.com
Asghar
Gafourian
Student Research Committee, Shahrekord University of Medical Science, Shahrekord, Iran.
gafourian@yahoo.com
10.22127/rjp.2020.208324.1534
<br /><strong><span class="s17"><span class="bumpedFont20">Background and objectives</span></span></strong><span class="s4"><span class="bumpedFont20"><strong>:</strong> Human adenoviruses cause a wide range of diseases, from self-limiting and mild infections to some life-threatening infections. Many studies have shown that components derived from medicinal plants have antiviral activity. </span></span><span class="s18"><span class="bumpedFont20">Pistacia</span></span><span class="s4"><span class="bumpedFont20"> genus is rich in phenolic compounds and has antioxidant and antimicrobial effects. The aim of this study was to investigate antioxidant potential and antiviral effects of ethanol and crude extracts and different fractions of </span></span><span class="s18"><span class="bumpedFont20">Pistacia</span></span><span class="s18"><span class="bumpedFont20"> </span></span><span class="s18"><span class="bumpedFont20">atlantica</span></span><span class="s4"><span class="bumpedFont20"> </span></span><span class="s4"><span class="bumpedFont20">Desf</span></span><span class="s4"><span class="bumpedFont20">. leaves on adenovirus. </span></span><strong><span class="s17"><span class="bumpedFont20">Methods:</span></span></strong><span class="s4"><span class="bumpedFont20"><strong> </strong>Crude</span></span><span class="s18"><span class="bumpedFont20"> P</span></span><span class="s4"><span class="bumpedFont20">.</span></span><span class="s18"><span class="bumpedFont20"> </span></span><span class="s18"><span class="bumpedFont20">atlantica</span></span><span class="s4"><span class="bumpedFont20"> leaf extract was prepared by maceration with 80% ethanol. Hexane, chloroform, ethyl acetate and n-butanol fractions were prepared using liquid-liquid extraction method. Toxicity on HEp-2 cells and anti-adenoviral activity of the extract/fractions were evaluated by MTT colorimetric methods. The concentration that caused 50% viral inhibition (IC</span></span><span class="s19"><span class="bumpedFont20">50</span></span><span class="s4"><span class="bumpedFont20">) and 50 % cytotoxicity concentration (CC</span></span><span class="s19"><span class="bumpedFont20">50</span></span><span class="s4"><span class="bumpedFont20">) were evaluated using regression analysis. Selectivity index (SI), as a marker of antiviral activity, was calculated. To determine antioxidant activity the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay was used. </span></span><strong><span class="s17"><span class="bumpedFont20">Results:</span></span></strong><span class="s4"><span class="bumpedFont20"><strong> </strong>Ethyl acetate fraction showed the highest antioxidant activity with IC</span></span><span class="s19"><span class="bumpedFont20">50</span></span><span class="s4"><span class="bumpedFont20">of 1.54±0.12 </span></span><span class="s4"><span class="bumpedFont20">μg</span></span><span class="s4"><span class="bumpedFont20">/mL in DPPH scavenging assay. Based on our results, IC</span></span><span class="s19"><span class="bumpedFont20">50</span></span><span class="s4"><span class="bumpedFont20"> of </span></span><span class="s18"><span class="bumpedFont20">P. </span></span><span class="s18"><span class="bumpedFont20">atlantica</span></span><span class="s4"><span class="bumpedFont20"> crude extract on adenovirus was 15.72 </span></span><span class="s4"><span class="bumpedFont20">μg</span></span><span class="s4"><span class="bumpedFont20">/mL, with SI of 8.09; n-butanol fraction showed the highest anti-adenoviral activity among the fractions with IC</span></span><span class="s19"><span class="bumpedFont20">50</span></span><span class="s4"><span class="bumpedFont20">of 16.38 µg/mL and SI of 26.5. </span></span><strong><span class="s17"><span class="bumpedFont20">Conclusion:</span></span></strong><span class="s4"><span class="bumpedFont20"> The ethanol extract and n-butanol fraction of </span></span><span class="s18"><span class="bumpedFont20">P. </span></span><span class="s18"><span class="bumpedFont20">atlantica</span></span><span class="s4"><span class="bumpedFont20"> leaves showed inhibitory effects on adenovirus and could be a new promising anti-adenovirus agent.</span></span>
Adenovirus,Antioxidant effect,Antiviral,Pistacia atlantica Desf
https://www.rjpharmacognosy.ir/article_104314.html
https://www.rjpharmacognosy.ir/article_104314_8cf1aeee1de53396336a4eb1447a24fb.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
Potential of Trachyspermum ammi (ajwain) Gel for Treatment of Facial Acne vulgaris: a Pilot Study with Skin Biophysical Profile Assessment and Red Fluorescence Photography
61
69
EN
Ziba
Talebi
0000-0003-4526-3384
International Campus, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
ziba.talebi@yahoo.com
Gholamreza
Kord Afshari
0000-0001-9052-9626
Department of Iranian Traditional Medicine, School of Iranian Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
kordafsharigh@gmail.com
Saman
Ahmad Nasrollahi
0000-0002-7384-6686
Center for Research & Training in Skin Diseases & Leprosy, Tehran University of Medical Sciences, Tehran, Iran
snasrollahi@tums.ac.ir
Alireza
Firooz
0000-0001-7274-4840
Center for Research & Training in Skin Diseases & Leprosy, Tehran University of Medical Sciences, Tehran, Iran
firozali@tums.ac.ir
Maedeh
Ghovvati
0000-0002-9371-0508
International Campus, School Of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
maedeh.ghovvati@yahoo.com
Aniseh
Samadi
0000-0002-0134-429X
Center for Research & Training in Skin Diseases & Leprosy, Tehran University of Medical Sciences, Tehran, Iran.
aniseh_samadi@yahoo.com
Mehrdad
Karimi
0000-0001-5395-819X
Department of Iranian Traditional Medicine, School of Iranian Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
mehrdadkarimi@yahoo.com
Sima
Kolahdooz
https://orcid.org/0
Department of Iranian Traditional Medicine, School of Iranian Traditional Medicine, Tehran University of Medical Sciences, Tehran, Iran.
simakolahdooz@yahoo.com
Mahdi
Vazirian*
0000-0002-8386-2225
Department of Pharmacognosy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Medicinal Plants Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
10.22127/rjp.2020.104701
<strong><span class="s20"><span class="bumpedFont20">Background and objectives: </span></span></strong><span class="s4"><span class="bumpedFont20">Acne vulgaris is one of the most common dermatologic conditions</span></span><span class="s21"><span class="bumpedFont20">.</span></span><span class="s4"><span class="bumpedFont20"> The available anti-acne treatments are not satisfactory and safe. In this regard, searching for new treatments, especially natural materials </span></span><span class="s4"><span class="bumpedFont20">with reasonable side effects and satisfactory effectiveness</span></span><span class="s4"><span class="bumpedFont20">, could be promising. The aim of the present study was to explore the safety and efficacy of a topical formulation containing </span></span><span class="s22"><span class="bumpedFont20">Trachyspermum ammi</span></span><span class="s4"><span class="bumpedFont20"> (ajwain)</span></span><span class="s20"><span class="bumpedFont20"> </span></span><span class="s4"><span class="bumpedFont20">fruits essential oil in patients with facial acne. </span></span><strong><span class="s20"><span class="bumpedFont20">Methods:</span></span></strong><span class="s4"><span class="bumpedFont20"><strong> </strong>The essential oil of the fruits </span></span><span class="s4"><span class="bumpedFont20">was</span></span><span class="s4"><span class="bumpedFont20"> extracted by hydrodistillation method and formulated as a 1% gel</span></span><span class="s4"><span class="bumpedFont20">.</span></span><span class="s4"><span class="bumpedFont20"> </span></span><span class="s4"><span class="bumpedFont20">In this open-labeled, uncontrolled clinical trial, 20 patients with mild to moderate acne received topical ajwain gel twice daily for 8 weeks. The outcomes of acne lesion count, red fluorescence parameters and biophysical skin profiles were evaluated at baseline, 4</span></span><span class="s23"><span class="bumpedFont20">th</span></span><span class="s4"><span class="bumpedFont20"> and 8</span></span><span class="s23"><span class="bumpedFont20">th</span></span><span class="s4"><span class="bumpedFont20"> weeks. Any adverse reaction was recorded during the study. </span></span><strong><span class="s20"><span class="bumpedFont20">Results: </span></span></strong><span class="s4"><span class="bumpedFont20">All patients completed the study. Two months after treatment, the mean reduction in the total (8.2±3.36; </span></span><span class="s22"><span class="bumpedFont20">P</span></span><span class="s4"><span class="bumpedFont20">=0.000) and non-inflammatory (7.3±4.53; </span></span><span class="s22"><span class="bumpedFont20">p</span></span><span class="s4"><span class="bumpedFont20">=0.000) lesions was statistically significant. Furthermore, a significant reduction in the size and quantity of red fluorescence spots was also observed. Biophysical skin profile measurements revealed a significant reduction in erythema (</span></span><span class="s22"><span class="bumpedFont20">p</span></span><span class="s4"><span class="bumpedFont20">=0.033) and sebum (</span></span><span class="s22"><span class="bumpedFont20">p</span></span><span class="s4"><span class="bumpedFont20">=0.026) and a significant increase in pH (</span></span><span class="s22"><span class="bumpedFont20">p</span></span><span class="s4"><span class="bumpedFont20">=0.005). No serious adverse events were reported. </span></span><strong><span class="s20"><span class="bumpedFont20">Conclusion: </span></span></strong><span class="s4"><span class="bumpedFont20">The results of this pilot study provided a basis for the effectiveness of topical ajwain gel for the treatment of mild to moderate facial acne. Conducting further double blind clinical trials are necessary to confirm the efficacy and safety of the product.</span></span><br /> <span> </span>
acne vulgaris, ajwain,Clinical trial,Essential oil,Trachyspermum ammi
https://www.rjpharmacognosy.ir/article_104701.html
https://www.rjpharmacognosy.ir/article_104701_6ea4369261fb73556e253bede2abdf45.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
Persian Asafoetida vs. Sagapenum: Challenges and Opportunities
71
80
EN
Alireza
Barzegar
0000-0003-4696-0720
Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
barzegarar@sums.ac.ir
Mohammad Amin
Salim
Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
aminsalim92@gmail.com
Parmis
Badr
0000-0001-9136-6785
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
Phytopharmaceutical Technology and Traditional Medicine Incubator, Shiraz University of Medical Sciences, Shiraz, Iran.
badrp@sums.ac.ir
Ahmadreza
Khosravi
Department of Biology, School of Science, Shiraz University, Shiraz, Iran.
Shiva
Hemmati
Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
hemmatish@sums.ac.ir
Hassan
Seradj
Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Mehrdad
Iranshahi
Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
Abdolali
Mohagheghzadeh*
0000-0002-4873-1449
Department of Phytopharmaceuticals (Traditional Pharmacy), School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
abdolalimohagheghzadeh@gmail.com
10.22127/rjp.2019.196452.1516
Asafoetida and sagapenum as valuable Iranian oleo-gum-resins from <em>Ferula </em>spp. (<em>F. assa-foetida</em>, <em>F. persica</em>, <em>F. foetida</em> and <em>F. alliacea</em>) have received interest during the history for producing valuable perfumes and common spices or as pharmaceutical agents. The aim of the present work was to characterize sources of asafoetida and sagapenum, according to botanical aspects, traditional and conventional medicine and phytochemical data. Available publications have been gathered from databases up to May 2019, and common Persian literatures were reviewed. We showed that sources for asafoetida and sagapenum should be differentiated botanically according to basal leaves, size of rays, fruits and petals. Furthermore, despite similarities, volatile sulfur components in <em>F. assa-foetida</em> are disulphides, in <em>F. persica</em> tri/tetra/pentasulphides, and in <em>F. alliacea</em> trisulphides. In the case of coumarins, conferol, conferone, farnesiferol A and B, samarcandin and samarcandin acetate are so far reported from <em>F. assa-foetida</em> and <em>F. persica</em>, and asacoumarin A from <em>F. assa-foetida</em> and <em>F. foetida</em> samples; while, persicaosides A-D have been reported only from<em> F. persica</em>. Moreover, farnesiferol C as the major coumarin in <em>F. assa-foetida</em> and mogoltavidin in <em>F. persica</em> would be new pharmacopeia markers. However, coumarins and essential oils similarities and differences among <em>Ferula</em> species should be more studied in a comparative research. Such studies would be essential for determining reliable sources and minimum requirements for standardizations of worldwide valuable wild growing medicinal and economical plants.
asafoetida,Ferula alliacea,Ferula assa-foetida,Ferula foetida,Ferula persica
https://www.rjpharmacognosy.ir/article_97393.html
https://www.rjpharmacognosy.ir/article_97393_c6e9b34cf2d4775e072d84055b1c1ea3.pdf
- The Iranian Society of Pharmacognosy
- Shahid Beheshti University of Medical Sciences
Research Journal of Pharmacognosy
2345-4458
2345-5977
7
2
2020
04
01
Internal Septum of Walnut Kernel: a Neglected Functional Food
81
92
EN
Zahra
Ghiravani
0000-0002-5717-4445
Department of Physiology, Faculty of Medicine, Birjand University of Medical Sciences, Birjand, Iran.
ghiravani@yahoo.com
Mohammadmehdi
Hassanzadeh-Taheri
Cellular and Molecular Research Center, Department of Anatomical Sciences, Birjand University of Medical Sciences, Birjand, Iran.
mmhtahery35@gmail.com
Mahsa
Hassanzadeh-Taheri
Student Research Committee, Medical Student, Birjand University of Medical Sciences, Birjand, Iran.
mah_hassanzadeh@yahoo.com
Mehran
Hosseini
0000-0002-6793-2035
Cellular and Molecular Research Center, Department of Anatomical Sciences, Birjand University of Medical Sciences, Birjand, Iran.
mehranhosseiny@yahoo.co.in
10.22127/rjp.2020.203451.1522
Walnut (<em>Juglans regia </em>L.) is a well-known member of the Juglandaceae family and its kernel is widely consumed around the world for both unique nutritional characteristics and health-related benefits. Even though several studies investigated the composition and biological activities of different parts of the walnut tree, the internal septum of the walnut kernel is less evaluated. In the last two decades, some studies investigated phytochemical and pharmacological aspects of the walnut septum. Their results showed a wide range of biological properties along with safety of walnut septum constituents convincing us to shift our view to walnut septum as a useless by-product to a natural herbal material with valuable properties. The purpose of this review was to summarize the currently available investigations on chemical composition and biological activities of the walnut septum. Several phytochemical studies showed that the walnut septum is a rich source of secondary metabolites like polyphenols are which estimated to be responsible for its high antioxidant property. Further experimental studies confirmed many biological activities of this by-product such as radical scavenging, food preservative, antibacterial, antitumor, hypoglycemic, hypolipidemic, and hepatorenal protective properties.
Juglandaceae,Juglans regia,Walnut
https://www.rjpharmacognosy.ir/article_101507.html
https://www.rjpharmacognosy.ir/article_101507_f0448255245bdda20b116bf44c49fffe.pdf