ORIGINAL_ARTICLE
Antibacterial and Antioxidant Activity of Cirsium englerianum (Asteraceae), an Endemic Plant to Ethiopia
Background and objectives: Cirsium englerianum (Asteraceae) is an endemic medicinal plant to Ethiopia. It is used to treat skin infection, snake bite and cough. The aim of the present study was to evalute the bioactivity of root extracts of C. englerianum. Methods: Phythochemical screening tests were employed by standard protocols to identifiy the phythochemicals. Column chromatographic separation was used to isolate the compounds and the spectroscopic techniques (IR, NMR and ESMS) were used to elucidtae structures of the compounds. Disc diffusion technique was uesd to evalute antibacterial activity. In vitro antioxidant activity was assessed by 1, 1-diphenyl-2-picrylhydrazyl (DPPH), and phosphomolybdenun assays. The total flavonoids content was determined by aluminium chloride method. Results: Phytochemical screening tests revealed presence of alkaloids, steroids, terpenoids, tannins, and flavonoids in the acetone root extract. Column chromatographic separation of chloroform/methanol (1:1) extract offered stigmasterol (1), and stigmasteryl stearate (2). The acetone extract was potentially effective against the tested bacterial strains (Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella typhi) at all concentrations (25, 50 and 100 mg/mL). In vitro antioxidant activity attributed that the acetone extract showed DPPH scavenging (IC50 =154.44±74 µg/mL) and total antioxidant activity (8.24±0.9 mg of ascorbic acid equivalent per gram of dry extract). The total flavonoid content was observed in the range from 5.88 ±0.21 to 8.24±0.9 milligrams of catechin equivalents per gram of dry plant extract. Conclusion: Stigmasterol and stigmasteryl stearate were reported for the first time from this plant. The results proved that acetone extract exhibited potential antibacterial and antioxidant activity which correlated with inhibition zone diameter, and free radical scavenging activity.
https://www.rjpharmacognosy.ir/article_129935_44058b577e6b7ddb7df3a12da65f573d.pdf
2021-07-01
5
12
10.22127/rjp.2021.273419.1680
Antibacterial Activity
Antioxidant activity
Cirsium englerianum
Phytochemical
Mesfin
Bibiso*
mefbab2009@gmail.com
1
Department of Chemistry, College of Natural and Computational Science, Wolaita Sodo University, Sodo, Ethiopia.
AUTHOR
Mathewos
Anza
mathewosanza@gmail.com
2
Department of Chemistry, College of Natural and Computational Science, Wolaita Sodo University, Sodo, Ethiopia.
AUTHOR
Bereket
Alemayehu
bereketalemayehu@gmail.com
3
Department of Biology, College of Natural and Computational Science, Wolaita Sodo University, Sodo, Ethiopia.
AUTHOR
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22
ORIGINAL_ARTICLE
Harmine Mitigates Liver Injury Induced by Mercuric Chloride via the Inhibition of Oxidative Stress
Background and objective: The mercury-induced liver pathogenesis is mainly mediated by oxidative stress. The aim of the current study was to evaluate the possible ameliorative effect of harmine, a natural compound, on liver toxicity induced by mercury chloride (HgCl2). Methods: Forty-two male Balb/c mice were randomly divided into six groups (n = 7): Control, HgCl2 (0.5 mg/kg), harmine (20 mg/kg), and HgCl2 (0.5 mg/kg) + harmine (5, 10, or 20 mg/kg). The mice received treatments once per day for two weeks. After this period, the blood and tissue samples were collected for analyses. Results: HgCl2 caused a significant increase in levels of hepatic enzymes alanine aminotransferase, aspartate transaminase, and alkaline phosphatase; while harmine ameliorated these effects. Harmine in HgCl2-intoxicated mice, showed protective effects as evidenced by the increase in liver relative weight to body as well as the diameter of central vein in the co-treated group. Serum levels of malondialdehyde and nitric oxide increased in HgCl2, while they were declined in harmine co-treated groups compared to HgCl2 group. The serum level of superoxide dismutase and total antioxidant capacity improved following harmine treatment in the co-administrated group compared to HgCl2 group. Moreover, gene expression analysis demonstrated that harmine treatment improved the HgCl2-induced decreasing of Ho-1, Nrf2, Hqo1, and Trx1. The histopathological examination confirmed the protective effects of harmine. Conclusion: Mercury can induce toxicity by elevation of oxidative stress in the liver and harmine attenuates hepatic injury induced by HgCl2, at least in part, through its antioxidant activities.
https://www.rjpharmacognosy.ir/article_129079_9f853d28aac6d1a2926495e0cc6d5aa7.pdf
2021-07-01
13
23
10.22127/rjp.2021.262620.1653
Harmine
hepatotoxicity
Liver
mercuric chloride
oxidative stress
Cyrus
Jalili
cjalili@kums.ac.ir
1
Department of Anatomy, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
AUTHOR
Sara
Darakhshan
darakhshan.sara@gmail.com
2
Pharmaceutical Sciences Research Center, Health Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran.
AUTHOR
Mohammadreza
Azimi
rezazimi87@gmail.com
3
Department of Biochemistry, Medical Faculty, Saveh Branch, Islamic Azad University, Saveh, Iran.
AUTHOR
Ali
Ghanbari*
aghanbari@kums.ac.ir
4
Department of Anatomy, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
AUTHOR
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42
ORIGINAL_ARTICLE
Assessment of Molecular Mechanism of Saffron Anti-Stress Property
Background and objectives: There are several documents about protective properties of saffron against stress conditions which refer to the effect of saffron on gene expression pattern of the treated samples.The aim of the present study was determination of the main regulated proteins by saffron extract. Methods: Twenty differentially expressed proteins from a published research were investigated via network analysis and assessed to determine the crucial regulated individuals by Cetoscape software. The network was analyzed by network analyzer application of Cytoscape software, and the central nodes were identified. Results: Twenty queried proteins were included in a network with 9005 nodes and 11446 edges. Analysis of the network revealed that VCP, SOD1, GRP78 (HSPA5), GRP75 (HSPA9), PRDX1, PHB, COMT, and ATP5H are the central proteins which are regulated by saffron extract. Conclusion: Based on the regulated proteins, regulation of mithoconderia and endoplasmic reticulum was identified as the main target of saffron in stress management.
https://www.rjpharmacognosy.ir/article_129218_197db16dc31ef51ac30bce1f806c8f17.pdf
2021-07-01
25
31
10.22127/rjp.2021.266106.1660
Bioinformatics
Network analysis
Protein expression
Rat
Saffron
Babak
Arjmand
1
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Mahmood
Khodadoost
khodadost.m@gmail.com
2
Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Mohhamadreza
Razzaghi
3
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Mostafa
Rezaei Tavirani*
tavirany@yahoo.com
4
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Alireza
Ahmadzadeh
5
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Sina
Rezaei Tavirani
6
Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
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3
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33
ORIGINAL_ARTICLE
Holothurin B Isolated from Holothuria atra Inhibits Angiogenesis More Potent than Curcumin in Vitro
Background and objectives: Triterpene glycosides as the most bioactive components of sea cucumbers, have been considered for their various pharmacological properties especially anticancer and anti-metastasis activities. Due to the limited information on the biological properties of holothurin B as a marine triterpene glycoside, the present study aimed to examine its effect on angiogenesis and compare it with curcumin usinghuman umbilical vein endothelial cells (HUVECs). Methods: Holothurin B was isolated from Holothuria atra and identified by NMR and Mass spectroscopic data. Cell survival was estimated using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) technique and migration of cells was assessed by Transwell test. Angiogenesis was evaluatedin vitro by tube formation assay. Results: Holothurin B reduced HUVECssurvival with IC50 value of 8.16 µg/mL. At the concentrations of 5 and 7.5 µg/mL, it significantly decreased the number of migrated cells, the average length and size of tubules, and mean number of junctions; it was more potent than curcumin. Conclusion: Holothurin B could be considered as a potent antiangiogenic constituent through suppressing endothelial cell proliferation, migration and tubulogenesis in vitro, suggesting its potential for further animal and clinical investigations.
https://www.rjpharmacognosy.ir/article_130034_295f1fe86421bcce0f9aab21a2117316.pdf
2021-07-01
33
40
10.22127/rjp.2021.277328.1687
angiogenesis inhibitors
cell migration inhibition
Cell proliferation
holothurin B
human umbilical vein endothelial cells
Afsaneh
Yegdaneh
yekdaneh@pharm.mui.ac.ir
1
Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Leila
Safaeian*
leila_safaeian@pharm.mui.ac.ir
2
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
LEAD_AUTHOR
Mina
Mirian
mina.mirian@pharm.mui.ac.ir
3
Department of Pharmaceutical Biotechnology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Nasim
Dana
dana.nasim@gmail.com
4
Applied Physiology Research Center, Cardiovascular Research Institute, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Mohadeseh
Taheri
mohadeseh2t@gmail.com
5
Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
[1] Iranmanesh M, Mohebbati R, Forouzanfar F, Roshan MK, Ghorbani A, Nik MJ, Soukhtanloo M. In vivo and in vitro effects of ethanolic extract of Trigonella foenum-graecum L. seeds on proliferation, angiogenesis and tube formation of endothelial cells. Res Pharm Sci. 2018; 13(4): 343–352.
1
[2] Bielenberg DR, Zetter BR. The contribution of angiogenesis to the process of metastasis. Cancer J. 2015; 21(4): 267–273.
2
[3] Tehranifard A, Rahimibashar MR. Description a sea cucumber spesies Holothuria atra Jaeger, 1833 from Kish Island Iran (Echinodermata: Holothuridea). J Basic Appl Sci Res. 2012; 2(12): 12660–12664.
3
[4] Grauso L, Yegdaneh A, Sharifi M, Mangoni A, Zolfaghari B, Lanzotti V. Molecular networking-based analysis of cytotoxic saponins from sea cucumber Holothuria atra. Mar Drugs. 2019; 17(2): 1–13.
4
[5] Dhinakaran DI, Lipton AP. Bioactive compounds from Holothuria atra of Indian Ocean. Springer Plus. 2014; 3(1): 1–10.
5
[6] Shahinozzaman M, Ishii T, Takano R, Halim MA, Hossain MA, Tawata S. Cytotoxic desulfated saponin from Holothuria atra predicted to have high binding affinity to the oncogenic kinase PAK1: a combined in vitro and in silico study. Sci Pharm. 2018; 86(3): 1–13.
6
[7] Zhao Q, Xue Y, Liu ZD, Li H, Wang JF, Li ZJ, Wang YM, Dong P, Xue CH. Differential effects of sulfated triterpene glycosides, holothurin A1, and 24-dehydroechinoside A, on antimetastasic activity via regulation of the MMP-9 signal pathway. J Food Sci. 2010; 75(9): 280–288.
7
[8] Zhao Q, Xue C, Zhang X, Dong P, Wang Y, Wang J. Antiangiogenesis effect of holothurin A1 and 24-dehydroechinoside A from Pearsonothuria graeffei. Chin Pharmacol Bull. 2011; 27(7): 934–939.
8
[9] Singh N, Kumar R, Gupta S, Dube A, Lakshmi V. Antileishmanial activity in vitro and in vivo of constituents of sea cucumber Actinopyga lecanora. Parasitol Res. 2008; 103(2): 351–354.
9
[10] Aminin DL, Menchinskaya ES, Pisliagin EA, Silchenko AS, Avilov S, Kalinin VI. Anticancer activity of sea cucumber triterpene glycosides. Mar Drugs. 2015; 13(3): 1202–1223.
10
[11] Bhandarkar SS, Arbiser JL. Curcumin as an inhibitor of angiogenesis. Adv Exp Med Biol. 2007; 595: 185–195.
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[12] Liu D, Schwimer J, Liu Z, Woltering EA, Greenway FL. Antiangiogenic effect of curcumin in pure versus in extract forms. Pharm Biol. 2008; 46(10-11): 677–682.
12
[13] Mansouri K, Rasoulpoor S, Daneshkhah A, Abolfathi S, Salari N, Mohammadi M, Rasoulpoor S, Shabani S. Clinical effects of curcumin in enhancing cancer therapy: a systematic review. BMC Cancer. 2020; 20(1): 1–11.
13
[14] Akbari V, Zafari S, Yegdaneh A. Anti-tuberculosis and cytotoxic evaluation of the seaweed Sargassum boveanum. Res Pharm Sci. 2018; 13(1): 30–37.
14
[15] Varshosaz J, Sadri F, Rostami M, Mirian M, Taymouri S. Synthesis of pectin-deoxycholic acid conjugate for targeted delivery of anticancer drugs in hepatocellular carcinoma. Int J Biol Macromol. 2019; 139: 665–677.
15
[16] Dana N, Javanmard SH, Rafiee L. Antiangiogenic and antiproliferative effects of black pomegranate peel extract on melanoma cell line. Res Pharm Sci. 2015; 10(2): 117–124.
16
[17] Gotink KJ, Verheul HMW. Anti-angiogenic tyrosine kinase inhibitors: what is their mechanism of action? Angiogenesis. 2010; 13(1): 1–14.
17
[18] Yu S, Ye X, Huang H, Peng R, Su Z, Lian XY, Zhang Z. Bioactive sulfated saponins from sea cucumber Holothuria moebii. Planta Med. 2015; 81(2): 152–159.
18
[19] Wang J, Han H, Chen X, Yi Y, Sun H. Cytotoxic and apoptosis-inducing activity of triterpene glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 cells. Mar Drugs. 2014; 12(8): 4274–4290.
19
[20] Yan B, Li L, Yi YH, Tang HF, Sun P, Zhang SY. Study on bioactive triterpene glycosides of sea cucumber Holothuria scabra Jaeger. Acad J Second Mil Med Univ. 2005; 26: 626–631.
20
[21] Patil TD, Thakare SV. In silico evaluation of selected triterpene glycosides as a human DNA topoisomerase II alpha (α) inhibitor. Int J Pharm Pharm Sci. 2012; 4: 201–204.
21
[22] Manolova Y, Deneva V, Antonov L, Drakalska E, Momekova D, Lambov N. The effect of the water on the curcumin tautomerism: a quantitative approach. Spectrochim Acta A Mol Biomol Spectrosc.2014; 132: 815–820.
22
[23] Gururaj AE, Belakavadi M, Venkatesh DA, Marmé D, Salimath BP. Molecular mechanisms of anti-angiogenic effect of curcumin. Biochem Biophys Res Commun.2002; 297(4): 934–942.
23
ORIGINAL_ARTICLE
Anti-Inflammatory Effect of Pimpinella anisum Extract in a Mouse Model of Allergic Asthma
Background and Objectives: Allergic asthma is a chronic inflammatory disease of the airways which has become prevalent globally. There are reports about the immunomodulatory and antioxidant effects of Pimpinella anisum L. seeds; so, in this study, we explored the suppressive effects of aqueous P. anisum L. seeds extract on ovalbumin-induced asthma in a mouse model. Methods: The seeds were extracted with water and the extract was dried by freeze-drying method. Twenty-eight BALB/c male mice weighing 15–20 g were divided into four groups of seven animals. Ovalbumin was used to trigger allergic asthma in these animals. Negative and positive control mice received phosphate-buffered saline and ovalbumin, respectively. The remaining two groups were challenged with ovalbumin and then received budesonide and the seed extract, respectively. Thereafter, the eosinophils count and expression of IL-5, -13, and -33 were measured in bronchoalveolar lavage fluid of mice. Histopathological changes of the lung tissues were also analyzed. Results: Aqueous extract of P. anisum seeds hindered ovalbumin -stimulated asthmatic complications by declining eosinophils number and expression of IL-5, -13, and -33 in bronchoalveolar lavage fluid of mice. It also inhibited the hyperplasia of goblet cells, hypersecretion of mucus, and inflammation in peribronchial and perivascular spaces, which were consequences of ovalbumin exposure. The activity of the extract in suppressing inflammatory responses of asthma in our murine model was comparable to budesonide. Conclusion: Our data underscored the effect of aqueous P. anisum seeds on the suppression of inflammatory responses of allergic asthma, proposing a promising suggestion for the treatment of the disease.
https://www.rjpharmacognosy.ir/article_131368_cb32ba0d3db950dc8c1de028f724eb11.pdf
2021-07-01
41
49
10.22127/rjp.2021.280757.1689
Asthma
budesonide
Eosinophil
Ovalbumin
Pimpinella anisum
Tahereh
Dargahi
td.ilia91@gmail.com
1
Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Reza
Ilkhani
ilkhanir@sbmu.ac.ir
2
Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Azadeh
Ghiaee
aghiaee@itmrc.org
3
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Roya
Arbabtafti
r1382tafti@yahoo.com
4
Iranian Research Institute of Plant Protection, Agricultural Research Education and Extension, Tehran, Iran.
AUTHOR
Shirin
Fahimi
s.fahimi@sbmu.ac.ir
5
Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Seyyed Shamsadin
Athari
ss.athari@zums.ac.ir
6
Department of Immunology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
AUTHOR
Fatemeh
Jafari
fateme.jafari7541@gmail.com
7
Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Hanieh
Kashafroodi
hanieh.k@gmail.com
8
Traditional Medicine and Materia Medica Research Center (TMRC), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Rasool
Choopani*
rchoopani@sbmu.ac.ir
9
Department of Traditional Medicine, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
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1
[2] Shin NR, Lee AY, Park G, Ko JW, Kim JC, Shin IS, Kim JS. Therapeutic effect of Dipsacus asperoides C. Y. Cheng et T. M. Ai in ovalbumin-induced murine model of asthma. Int J Mol Sci. 2019; 20(8): 1–13.
2
[3] Shin NR, Kwon HJ, Ko JW, Kim JS, Lee IC, Kim JC, Kim SH, Shin IS. S-allyl cysteine reduces eosinophilic airway inflammation and mucus overproduction on ovalbumin-induced allergic asthma model. Int Immunopharmacol. 2019; 68(8): 124–130.
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[6] Shojaii A, Abdollahi Fard M. Review of pharmacological properties and chemical constituents of Pimpinella anisum. Isrn Pharm. 2012; Article ID 510795.
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[7] Shams Ardekani MR, Farjadmand F, Rahimi R. Makhzan al adviyeh and pointing to the scientific names of medicinal plants for the first time in a persian book. Trad Integr Med. 2018; 3(4): 186–195.
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[9] Aghili Shirazi MH. Qarabadin-e-kabir. Qom: Rehabilitation institute of natural medicine, 2011.
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[12] Fujimatu E, Ishikawa T, Kitajima J. Aromatic compound glucosides, alkyl glucoside and glucide from the fruit of anise. Phytochemistry. 2003; 63(5): 609–616.
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[14] Sadr S, Kaveh N, Choopani R, Kaveh S, Dehghan S. Effect of exercise on asthma from Iranian traditional medicine perspective. Trad Integr Med. 2019; 4(2): 84–90.
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[15] Mirheydar H. Herbal information: usage of plants in prevention and treatment of diseases. Tehran: Islamic Culture Press Center, 2001.
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[16] Athari SS, Pourpak Z, Folkerts G, Garssen J, Moin M, Adcock IM, Movassaghi M, Ardestani MS, Moazzeni SM, Mortaz E. Conjugated alpha-alumina nanoparticle with vasoactive intestinal peptide as a nano-drug in treatment of allergic asthma in mice. Eur J Pharmacol. 2016; 791(6): 811–820.
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[17] Khodadoost M, Rajabi S, Tebianian M, Ghahremani Z, Athari SS, Irani M, Dargahi T, Choopani R. Alleviating effects of Lavandula aqueous extract on asthmatic complications in a mouse model. Comp Clin Pathol. 2021; 30(2): 199–206.
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[18] Mirzaei H, Akbari A, Goudarzi H. Silibinin upregulates e-cadherin expression in mkn-45 human gastric cancer cells. Res J Pharmacogn. 2018; 5(3): 41–46.
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[19] Irani M, Choopani R, Esmaeili S, Dargahi T, Athari SM, Athari SS. Effect of nettle seed on immune response in a murine model of allergic asthma. Rev Fr Allergol. 2020; 60(5): 417–422.
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[20] Shamshuddin NSS, Mohd Zohdi R. Gelam honey attenuates ovalbumin-induced airway inflammation in a mice model of allergic asthma. J Tradit Complement Med. 2018; 8(1): 39–45.
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[21] Cohn L, Elias JA, Chupp GL. Asthma: mechanisms of disease persistence and progression. Annu Rev Immunol. 2004; 22(1): 789–815.
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[22] Boskabady MH, Ramazani-Assari M. Relaxant effect of Pimpinella anisum on isolated guinea pig tracheal chains and its possible mechanism(s). J Ethnopharmacol. 2001; 74(1): 83–88.
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[23] Bejeshk MA, Samareh Fekri M, Najafipour H, Rostamzadeh F, Jafari E, Rajizadeh MA, Masoumi-Ardakani Y. Anti-inflammatory and anti-remodeling effects of myrtenol in the lungs of asthmatic rats: histopathological and biochemical findings. Allergol Immunopathol (Madr). 2019; 47(2): 185–193.
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[24] Barnes PJ. Pathophysiology of allergic inflammation. Immunol Rev. 2011; 242(1): 31–50.
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[25] Yadav UC, Naura AS, Aguilera-Aguirre L, Ramana KV, Boldogh I, Sur S, Boulares HA, Srivastava SK. Aldose reductase inhibition suppresses the expression of Th2 cytokines and airway inflammation in ovalbumin-induced asthma in mice. J Immunol. 2009; 183(7): 4723–4732.
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[26] Jeon J, Kim Y, Kim H, Kang JS, Lee WJ. Anti-inflammatory effect of alloferon on ovalbumin-induced asthma. Immune Netw. 2015; 15(6): 304–312.
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[27] Hwang YH, Hong SG, Mun SK, Kim SJ, Lee SJ, Kim JJ, Kang KY, Yee ST. The protective effects of astaxanthin on the ova-induced asthma mice model. Molecules. 2017; 22(11): 1–11 .
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[28] Athari SM, Mehrabi Nasab E, Athari SS. Study effect of Ocimum basilicum seeds on mucus production and cytokine gene expression in allergic asthma mice model. Rev Fr Allergol. 2018; 58(7): 489–493.
28
[29] Bakakos A, Loukides S, Bakakos P. Severe eosinophilic asthma. J Clin Med. 2019; 8(9): 1–24.
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[31] Liang Z, Xu Y, Wen X, Nie H, Hu T, Yang X, Chu X, Yang J, Deng X, He J. Rosmarinic acid attenuates airway inflammation and hyperresponsiveness in a murine model of asthma. Molecules. 2016; 21(6): 1–15.
31
[32] Theoharides TC, Alysandratos KD, Angelidou A, Delivanis DA, Sismanopoulos N, Zhang B, Asadi S, Vasiadi M, Weng Z, Miniati A, Kalogeromitros D. Mast cells and inflammation. Biochim Biophys Acta. 2012; 1822(1): 21–33.
32
[33] Huang CQ, Li W, Wu B, Chen WM, Chen LH, Mo GW, Zhang QF, Gong L, Li J, Zhang HC, Zhu HM, Zeng QZ. Pheretima aspergillum decoction suppresses inflammation and relieves asthma in a mouse model of bronchial asthma by nf-κb inhibition. J Ethnopharmacol. 2016; 189(2): 22–30.
33
[34] Qian J, Ma X, Xun Y, Pan L. Protective effect of forsythiaside a on ova-induced asthma in mice. Eur J Pharmacol. 2017; 812(1): 250–255.
34
[35] Lee HY, Hur J, Kim IK, Kang JY, Yoon HK, Lee SY, Kwon SS, Kim YK, Rhee CK. Effect of nintedanib on airway inflammation and remodeling in a murine chronic asthma model. Exp Lung Res. 2017; 43(5): 187–196.
35
[36] Thai P, Loukoianov A, Wachi S, Wu R. Regulation of airway mucin gene expression. Annu Rev Physiol. 2008; 70(3): 405–429.
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[37] Yang SH, Kao TI, Chiang BL, Chen HY, Chen KH, Chen JL. Immune-modulatory effects of Bu-Zhong-Yi-Qi-Tang in ovalbumin-induced murine model of allergic asthma. PloS One. 2015; 10(6): 1–12.
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[38] Ye Y, Mo S, Feng W, Ye X, Shu X, Long Y, Guan Y, Huang J, Wang J. The ethanol extract of Involcucrum castaneae ameliorated ovalbumin-induced airway inflammation and smooth muscle thickening in guinea pigs. J Ethnopharmacol. 2019; 230: 9–19.
38
ORIGINAL_ARTICLE
Flavonoids and Phenolics Contents, Antioxidant and Antibacterial Potential of Folk Medicinal Plants Used in Northeastern Thailand
Background and objectives: Thailand has abundant traditional medicinal plant species which are efficacious for many illnesses, but most of them still lack the supportive scientific information for their healing properties. The aim of this study was to evaluate and compare the constituents and antioxidant and antibacterial activities of some of these plants. Methods: The medicinal plant extracts were assessed for their flavonoids and phenolics composition and tested for antibacterial activity using disk diffusion method. In vitroantioxidant capacity was evaluated by DPPH, ABTS, and FRAP assays. Results: Major flavonoids present in the medicinal plants were naringenin, (+)-catechin and quercetin. The highest contents of naringenin, quercetin and (+)-catechin were observed in Tinospora crispa (896.15 mg/100 g dw), Betula alnoides (521.57 mg/100 g dw) and Albizia procera (430.28 mg/100 g dw), respectively (P<0.05). Naringenin was first reported from T. crispa,quercetin and (-)-epicatechin were also found in this plant. The lowest EC50 value based on the DPPHassay was found in Capparis micracantha extracts (9.10 mg/mL). The strongest antioxidant capacities, examined by the DPPH, FRAP and ABTS assays, were found in Capparis micracantha (EC50 9.10 mg/mL), Zingiber cassumunar (334.00 mg Fe(II)/100 g dw) and Plumbago indica (61.56 mg TE/100 g dw), respectively (p<0.05). The extract of Plumbago indica root exhibited the highest antibacterial activity mainly against Bacillus subtilis (MIC = 1.56 mg/mL), Bacillus cereus (MIC = 0.39 mg/mL), Streptococcus faecalis (MIC = 0.19 mg/mL), Salmonella sp. (MIC = 0.39 mg/mL) and Salmonella typhi (MIC = 0.19 mg/mL). Conclusion: The results provided significant scientific data on phytochemical constituents and biological activities of Thai medicinal plants use in traditional medicine and the relation to their therapeutic properties.
https://www.rjpharmacognosy.ir/article_131369_b4cb75ca65a73c5abe1c7a1f85e923d6.pdf
2021-07-01
51
65
10.22127/rjp.2021.276097.1685
Antioxidant activity
Antibacterial Activity
Flavonoids
folk medicinal plants
Wannee
Samappito
wannanoi@yahoo.com
1
Department of Food Technology, Faculty of Technology, Mahasarakham University, Mahasarakham, Thailand.
AUTHOR
Sujitar
Jorjong
sujitar_9@hotmail.com
2
Department of Plant Science, Faculty of Natural Resource, Rajamangala University of Technology, Isan Sakonnakhon Campus, Sakon Nakhon, Thailand.
AUTHOR
Luchai
Butkhup*
tak_biot2000@hotmail.com
3
Natural Antioxidant Innovation Research Unit (NAIRU), Department of Biotechnology, Faculty of Technology, Mahasarakham University, Mahasarakham, Thailand.
AUTHOR
[1] Tyagi B, Dubey A, Verma AK, Tiwari S. Antibacterial activity of phenolics compounds against pathogenic bacteria. Int J Pharm Sci Rev Res. 2015; 35(1): 16-18.
1
[2] Sharma I, Rangra A, Tripathi A. Comparative analysis of antimicrobial, antioxidant and phytochemicals from the folk medicinal plants of Dharampur, Solan. Annals Pharm Pharm. 2019; 4(1): 1–7.
2
[3] Guldiken B, Ozkan G, Catalkaya G, Ceylan FD, Yalcinkaya IE, Capanoglu E. Phytochemicals of herbs and spices: health versus toxicological effects. Food Chem Toxicol. 2018; 119: 37-49.
3
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4
[5] Sivakrishnan S, Pharm M, Kavitha J. An overview on benefits of Albizia procera. Int J Res Anal Rev. 2018; 5(1): 224-230.
5
[6] Lepcha SR, Das AP. Ethno-medicobotanical exploration along the international borders to Tibet Autonomous Region of China and the kingdom of Bhutan with special reference to the Pangolakha Wildlife Sanctuary. Proceedings of the national seminar on the exploration, protection and conservation of biodiversity and traditional knowledge. 2011 Feb 4-5, Kolkata, India.
6
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[22] Ozaki Y, Kawahara N, Harada M. Anti-inflammatory effect of Zingiber cassumunar Roxb. and its active principles. Chem Pharm Bull. 1991; 39(9): 2353-2356.
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[23] Gulluce M, Sahin F, Sokmen M, Ozer H, Daferera D, Sokmen A, Polissiou M, Adiguzel A, Ozkan H. Antimicrobial and antioxidant properties of the essential oils and methanol extract from Mentha longifolia L. ssp. longifolia. Food Chem. 2007; 103(4): 1449-1456.
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[32] Nahak G, Sahu RK. Phytochemical evaluation and antioxidant activity of Piper cubeba and Piper nigrum. J Appl Pharm Sci. 2011; 1(8): 153-157.
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44
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45
ORIGINAL_ARTICLE
The Noradrenergic System is Partly Involved in Resveratrol Antidepressant and Anti-Obsessive Like Effects in Mice Model
Background and objectives: Resveratrol is a natural phenol in food particularly the skin of fruits like red grapes. It has shown biological, and antidepressant effects. The objective of the present study was to evaluate the role of adrenergic system on antidepressant and anti-obsessive effect of resveratrol. Methods: Male mice (weighing 27±2 g) were used. A tyrosine hydroxylase inhibitor, α-methyl-p-tyrosine (AMPT 100 mg/kg), α1 adrenergic receptors (AR) antagonist (prazosin, 1 mg/kg), α2-AR antagonist (yohimbine, 1 mg/kg), β-AR antagonist (propranolol, 2 mg/kg) and a tricyclic antidepressant (imipramine, 5 mg/kg), were injected before resveratrol (60 mg/kg). Locomotor activity, burring behavior during marble burring test, and immobility time during forced swimming test (FST) were evaluated. Results: No significant difference was observed in the locomotor activity between groups. The immobility time increased following pretreatment with AMPT (147.3±6.35s vs resveratrol alone 85.67±4.51s, p <0.001); marble burring behavior increased significantly, indicating the possible role of norepinephrine in resveratrol antidepressant and anti-obsessive-like effects. Propranolol (163.8±8.25 s, p <0.001) and yohimbine (151.0±6.47s, p=0.0030) pretreatment increased immobility in the FST compared to resveratrol. Pretreatment with prazosin did not cause important change in FST. Pretreatment with propranolol slightly increased marble burring behavior while no changes were observed following yohimbine or prazosin administration. Imipramine pretreatment did not have additive antidepressant effect with resveratrol and increased immobility time (136.1±16.88 s, p=0.014 vs resveratrol). Conclusion: Resveratrol antidepressant-like effect is partly mediated by the noradrenergic system, and interaction with β-AR and α2-AR. Additionally, resveratrol anti-obsessive-like property involves noradrenergic system but not the β or α-AR.
https://www.rjpharmacognosy.ir/article_130728_49dfecf36386de759fcb3c6b35f99c69.pdf
2021-07-01
67
75
10.22127/rjp.2021.277101.1686
adrenergic antagonists
alpha-methyltyrosine
depression
Norepinephrine
Resveratrol
Azadeh
Mesripour*
a_mesripour@yahoo.com
1
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
Fatemeh
Payandekhah
ft.payandeh@gmail.com
2
Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
AUTHOR
[1] Shigemura J, Ursano RJ, Morganstein JC, Kurosawa M, Benedek DM. Public responses to the novel 2019 coronavirus (2019-nCoV) in Japan: mental health consequences and target populations. Psychiatry Clin Neurosci. 2020; 74(4): 281–282.
1
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2
[3] Mesripour A, Almasi M. Flaxseed prevents interferon-alpha induced depressive behavior in mice: the α-linolenic acid is essential. Res J Pharmacogn. 2021; 8(1): 63–71.
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[4] Rafieian-Kopaei M. Medicinal plants and the human needs. J Herbmed Pharmacol. 2012; 1(1): 1–2.
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[5] Ogle WO, Speisman RB, Ormerod BK. Potential of treating age related depression and cognitive decline with nutraceutical approaches: a mini-review. Gerontology. 2013; 59(1): 23–31.
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[6] Hurley LL, Akinfiresoye L, Nwulia E, Kamiya A, Kulkarni AA, Tizabi Y. Antidepressant-like effects of curcumin in WKY rat model of depression is associated with an increase in hippocampal BDNF. Behav Brain Res. 2013; 239: 27–30.
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[9] Baur JA, Sinclair DA. Therapeutic potential of resveratrol: the in vivo evidence. Nat Rev Drug Discov. 2006; 5(6): 493–506.
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[13] Maletic V, Eramo A, Gwin K, Offord SJ, Duffy RA. The role of norepinephrine and its α-adrenergic receptors in the pathophysiology and treatment of major depressive disorder and schizophrenia: a systematic review. Front Psychiatry. 2017; 8: 1–12.
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[14] Hein L. Adrenoceptors and signal transduction in neurons. Cell Tissue Res. 2006; 326(2): 541–551.
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[15] Brocardo PS, Budni J, Kaster MP, Santos ARS, Rodrigues ALS. Folic acid administration produces an antidepressant-like effect in mice: evidence for the involvement of the serotonergic and noradrenergic systems. Neuropharmacology. 2008; 54(2): 464–473.
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[17] Xu Y, Wang Z, You W, Zhang X, Li S, Barish PA, Vernon MM, Du X, Li G, Pan J, Ogle WO. Antidepressant-like effect of trans-resveratrol: Involvement of serotonin and noradrenaline system. Eur Neuropsychopharmacol. 2010; 20(6): 405–413.
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[20] Mesripour A, Kaviyanpour M, Hajhashemi V. Antidepressant-like effect of minocycline in mice forced swimming test: minor involvement of the noradrenergic system. Thai J Pharm Sci. 2019; 43(3): 125–130.
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[21] Gu Z, Chu L, Han Y. Therapeutic effect of resveratrol on mice with depression. Exp Ther Med. 2019; 17(4): 3061–3064.
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28
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33
ORIGINAL_ARTICLE
Evaluation of Anticancer Effect of Ghost Pepper: A Bioinformatics Assessment
Background and objectives: Natural sources can be effective in treating diverse pathological conditions especially cancer. Molecular evaluations of pepper on renal cancer could provide further information about its anticancer property. Methods: To achieve a clear understanding of pepper effect on cancer cells, protein-protein interaction network analysis of differentially expressed proteins (DEPs) in human renal adenocarcinoma cells treated with ghost pepper were evaluated. Cytoscape V. 3.8.2 and its applications were applied to analyze the DEPs. Results: Centrality study showed CYCS and CAT as DEPs were the hub-bottlenecks that were essential for the network stability. Among the 10 introduced central proteins, eight individuals belonged to the added first neighbors from STRING database. The finding indicated that the main central proteins belonged to the first neighbors of the queried proteins and were involved in the anticancer activity. Conclusion: Analysis highlighted anticancer property of ghost pepper on the human renal adenocarcinoma cells and also antioxidant effect which was associated with catalase activity.
https://www.rjpharmacognosy.ir/article_128929_cc3ad7ef81f254fd490a626aeb94f75b.pdf
2021-07-01
77
82
10.22127/rjp.2021.267225.1666
Bioinformatics
Cell line
ghost pepper
human renal adenocarcinoma
Protein
Mona
Zamanian-Azodi
1
Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Mostafa
Rezaei-Tavirani
tavirany@yahoo.com
2
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Somayeh
Esmaeili
soesmaeili@yahoo.com
3
Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Vahid
Mansouri*
vm1343@yahoo.com
4
Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
Babak
Arjmand
5
Cell Therapy and Regenerative Medicine Research Center, Endocrinology and Metabolism Molecular Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Somayeh
Jahani-Sherafat
6
Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
AUTHOR
[1] Irshad S, Ashfaq A, Muazzam A, Yasmeen A. Antimicrobial and anti-prostate cancer activity of turmeric (Curcuma longa L.) and black pepper (Piper nigrum L.) used in typical Pakistani cuisine. Pak J Zool. 2017; 49(5): 1–5.
1
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[3] Spiller F, Alves MK, Vieira SM, Carvalho TA, Leite CE, Lunardelli A, Poloni JA, Cunha FQ, De Oliveira JR. Anti‐inflammatory effects of red pepper (Capsicum baccatum) on carrageenan‐and antigen‐induced inflammation. J Pharm Pharmacol. 2008; 60(4): 473–478.
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[4] Lee SC, Hwang IS, Choi HW, Hwang BK. Involvement of the pepper antimicrobial protein CaAMP1 gene in broad spectrum disease resistance. Plant Physiol. 2008; 148(2): 1004–1020.
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[6] Kamaruddin MF, Hossain MZ, Mohamed Alabsi A, Mohd Bakri M. The antiproliferative and apoptotic effects of capsaicin on an oral squamous cancer cell line of Asian origin, ORL-48. Medicina. 2019; 55(7): 1–12.
6
[7] Perla V, Nadimi M, Reddy R, Hankins GR, Nimmakayala P, Harris RT, Valluri J, Sirbu C, Reddy UK. Effect of ghost pepper on cell proliferation, apoptosis, senescence and global proteomic profile in human renal adenocarcinoma cells. PLoS One. 2018; 13(10): 1–27.
7
[8] Samykutty A, Shetty AV, Dakshinamoorthy G, Bartik MM, Johnson GL, Webb B, Zheng G, Chen A, Kalyanasundaram R, Munirathinam G. Piperine, a bioactive component of pepper spice exerts therapeutic effects on androgen dependent and androgen independent prostate cancer cells. PLoS One. 2013; 8(6): 1–11.
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[9] Zhu M, Yu X, Zheng Z, Huang J, Yang X, Shi H. Capsaicin suppressed activity of prostate cancer stem cells by inhibition of Wnt/β‐catenin pathway. Phytother Res. 2020; 34(4): 817–824.
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[10] Liu Y, Nair MG. Capsaicinoids in the hottest pepper Bhut Jolokia and its antioxidant and antiinflammatory activities. Nat Prod Commun. 2010; 5(1): 91–94.
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[11] Zamanian-Azodi M, Rezaei-Tavirani M. Investigation of health benefits of cocoa in human colorectal cancer cell line, HT-29 through interactome analysis. Gastroenterol Hepatol Bed Bench. 2019; 12(1): 67–73.
11
[12] Rezaei-Tavirani M, Tavirani MR, Azodi MZ. The bioinformatics aspects of gene screening of HT-29, human colon cell line treated with caffeic acid. Gastroenterol Hepatol Bed Bench. 2019; 12(3): 246–253.
12
[13] Zamanian-Azodi M, Rezaei-Tavirani M, Nejadi N, Oskouie AA, Zayeri F, Hamdieh M, Safaei A, Rezaei-Tavirani M, Ahmadzadeh A, Amouzandeh-Nobaveh A, Okhovatian F. Serum proteomic profiling of obsessive-compulsive disorder, washing subtype: a preliminary study. Basic Clin Neurosci. 2017; 8(4): 307–316.
13
[14] Azodi MZ, Rezaei-Tavirani M, Rezaei-Tavirani M. Identification of the key genes of autism spectrum disorder through protein-protein interaction network. Galen Med J. 2019; 8: 1–8.
14
[15] Zali MR, Azodi MZ, Razzaghi Z, Heydari MH. Gallbladder cancer integrated bioinformatics analysis of protein profile data. Gastroenterol Hepatol Bed Bench. 2019; 12(Suppl1): 66–73.
15
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16
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17
[18] Assenov Y, Ramírez F, Schelhorn SE, Lengauer T, Albrecht M. Computing topological parameters of biological networks. Bioinform. 2008; 24(2): 282–284.
18
[19] Heidari MH, Razzaghi M, Baghban AA, Rostami-Nejad M, Rezaei-Tavirani M, Azodi MZ, Zali A, Ahmadzadeh A. Assessment of the microbiome role in skin protection against uv irradiation via network analysis. J Lasers Med Sci. 2020; 11(3): 238–242.
19
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29
ORIGINAL_ARTICLE
A Review of Ethnopharmacology, Phytochemistry and Pharmacology of Cymbopogon Species
The genus Cymbopogon belongs to Poaceae family and contain about 54 species, commonly known as "lemongrass". Cymbopogon is a medicinal plant native to tropical and subtropical areas which is applied traditionally for its numerous properties including antirheumatic, antispasmodic, analgesic, antiseptic, hypotensive, antitussive and anticonvulsant ctivities, and as a treatment for gastrointestinal and nervous disorders and fever. The aims of this study were to discuss about current state of phytochemistry, pharmacology, and pharmacological effects of different species of Cymbopogon. Electronic databases including PubMed, Scopus, Cochrane library and Google Scholar were searched with the scientific name and the common name of the plant until November 2019. In spite of the small number of clinical investigations, Cymbopogon genus is widely evaluated for its phytochemistry, ethnopharmacology and biological activities. Monoterpenes specially geranial, citronellol and citral are the chief components of the essential oil. Biological activities including antioxidant, antibacterial, antiviral, insecticidal, anticancer, hepatoprotective activities as well as its effect on skin, urogenital, gastrointestinal, neuropsychological and cardiovascular systems are proved in cell lines and animal models. Extensive studies have been done on various biological activities of lemongrass; nevertheless, safety and efficacy of Cymbopogon species are not fully evaluated in human and further well-designed clinical trials are required to confirm preclinical findings.
https://www.rjpharmacognosy.ir/article_131366_7830a32b73b821e5efcd9c6c7757b77c.pdf
2021-07-01
83
112
10.22127/rjp.2021.275223.1682
citral
Diabetes Mellitus
Hyperlipidemia
Inflammation
lemongrass
Soodeh
Karami
karamisoodeh@gmail.com
1
Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Alireza
Yargholi
alirezayargholi@gmail.com
2
Department of Persian Medicine, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Seyede Nargess
Sadati Lamardi
n_sadati@tums.ac.ir
3
Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Samaneh
Soleymani
s.soleymani84@gmail.com
4
Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Laila
Shirbeigi
l.shirbeigi@yahoo.com
5
Department of Persian Medicine, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Roja
Rahimi*
rojarahimi@gmail.com
6
Department of Traditional Pharmacy, School of Persian Medicine, Tehran University of Medical Sciences, Tehran, Iran.
AUTHOR
Karami S, Shamshiri S, Abdollahi M, Rahimi R. An evidence-based review of medicinal plants used in traditional Persian medicine for treatment of osteoarthritis. Curr Drug Disc Technol. 2021; 18(2): 244–271.
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